New-onset glucose disorders in peritoneal dialysis patients: a meta-analysis and systematic review.

Background: Peritoneal dialysis (PD) patients are at high risk of developing glucose metabolism disturbance (GMD). The incidence and prevalence of new-onset GMD, including diabetes mellitus (DM), impaired glucose tolerance (IGT) and impaired fast glucose (IFG), after initiation of PD, as well as their correlated influence factors, varies among studies in different areas and of different sample sizes. Also, the difference compared with hemodialysis (HD) remained unclear.

[The effect of ginkgolic acids on multidrug resistance in oral squamous cell carcinoma].

Objective: Carboplatin (CBP)-resistant cell line (Tca8113/CBP) and pingyangmycin (PYM)-resistant cell line (Tca8113/PYM) were established in vitro. Ginkgolic acids' influence over multidrug resistance (MDR) of drug-resistant cells was discussed by ginkgolic acids coupled with chemotherapy drugs.

Methods: The expression of P-glycoprotein (P-gp) was detected by immunohistochemistry.

[Preparation and identification of a monoclonal antibody against human PD-L1].

Objective: To prepare mouse anti-human PD-L1 monoclonal antibodies (mAbs) and identify their biological characteristics.

Methods: BALB/c mice were immunized with recombinant GST-PDL1 protein,and the strain of hybridoma cell secreting anti-PD-L1 mAb was obtained. The specificity of anti-PD-L1 mAb was checked and evaluated with ELISA and Western blot.

[Effect of astragalus membranaceus on the proliferation, osteogenic capacity and structure of periodontal ligament cells in vitro].

Objective: To investigate the effect of Astragalus membranaceus (APS) on the proliferation, osteogenic capacity and structure of periodontal ligament cells (PDLCs) in vitro.

Methods: PDLCs were cultured in vitro with APS of 0.08, 0.

Hypoxia inducible factor 1α and hypoxia inducible factor 2α play distinct and functionally overlapping roles in oral squamous cell carcinoma.

Purpose: This study aimed to investigate the functional difference between hypoxia inducible factor (HIF)-1α and HIF-2α in oral squamous cell carcinomas (OSCC).

Experimental Design: We evaluated the correlations between HIF-1α and HIF-2α expression and the clinical-pathologic characteristics of 97 patients with OSCC by immunohistochemical staining. OSCC cell lines transfected with lentivirus encoding short hairpin RNA against HIF-1α/2α were used to investigate the HIF-1α/2α-dependent target genes.

[The expression of vascular endothelial growth factor-C in oral squamous cell carcinoma and its associations with angiogenesis, lymphangiogenesis and lymph node metastasis].

Objective: To study the expression of vascular endothelial growth factor-C (VEGF-C) in oral squamous cell carcinoma (OSCC) and its relation to angiogenesis, lymphangiogenesis, as well as lymph node metastasis.

Methods: Sixty-seven archival specimens from patients with oral squamous cell carcinoma were investigated, whose clinicopathologic data were completely conserved. Immunohistochemical staining was performed to detect the expression of VEGF-C, microvessel density (MVD), lymphatic vessel density (LVD).

Role of NGF in spared DRG following partial dorsal rhizotomy in cats.

Neuroplasticity occurs in the spinal cord in response to lesions, but less is known about the underlying mechanism. This investigation explored the role of intrinsic NGF in axonal sprouting of dorsal root ganglia (DRG) in cats subjected to unilateral removal of L1-L5, L7-S2 DRG, but leaving the L6 DRG (spared DRG) undamaged. The expression of mRNA and protein for NGF and TrkA increased significantly by using in situ hybridization histochemistry and immunohistochemistry.

[Expressional change of nitric oxide synthases in dorsal root ganglia of cats after selective dorsal rhizotomy].

Objective: To examine the expressional change of nitric oxide synthase (NOS) in the injured dorsal root ganglia (DRG) and the ipsilateral adjacent uninjured DRG after selective dorsal rhizotomy.

Methods: Immunochemical ABC method was used to detect the distribution of immunoreaction complex of NOS isoforms--nNOS and eNOS, and quantitative analysis was conducted to get the number of nNOS-immunoreactivity (nNOS-IR) neurons in normal DRG, dorsal rhizotomized DRG and spared DRG from adult cats on the 6th day after operation. This operating model was made by rhizotomizing unilateral L1-L5 dorsal roots and leaving L6 as a spared root.

[The neurotrophic effect of endogenous NT-3 from adult cat spared dorsal root ganglion on ganglionic neurons].

Objective: To investigate the neurotrophic effect of endogenous NT-3 from adult cat dorsal root ganglion (DRG) on ganglionic neurons.

Methods: Rhizotomy of bilateral L1, L3, L5 and L7 dorsal roots of cats was performed, leaving L2, L4 and L6 DRG as spared DRGs. The separate neurons of normal (control) DRG, spared DRG and anti-NT-3 antibody blocking DRG were cultured in vitro respectively.