Publications by authors named "Si-wu Fu"
Ribosome assembly factor URB1 is essential for ribosome biogenesis. However, its latent role in cancer remains unclear. Analysis of The Cancer Genome Atlas database and clinical tissue microarray staining showed that URB1 expression was upregulated in colorectal cancer (CRC) and prominently related to clinicopathological characteristics.
View Article and Find Full Text PDFObjective: To investigate the effect of clostridium difficile toxin A(TcdA) on the Rho GTPases and the cytoskeleton in K562 cells.
Methods: K562 cells were cultured in vitro with different concentration of TcdA.The effect of TcdA proliferation of cells was detected by MTT method after the K562 cells were stimulated with TcdA for 24,48 and 72h; the expression of cdc42, RhoA, Rac1 mRNA was assessed by RT-PCR; the changes of the microtubule, the microfilament were observed by confocal laser scanning microscopy.
[Study of dental caries and correlated factors of 12-year-old children in Dongxiang, Baoan and Yugu races].
Hua Xi Kou Qiang Yi Xue Za Zhi
October 2009
Article Synopsis
- The study aimed to assess dental caries and related factors among 12-year-old children from Dongxiang, Baoan, and Yugu races.
- Findings showed high caries prevalence rates: 40.52% for Dongxiang, 44.29% for Baoan, and 46.45% for Yugu, with Yugu children displaying better oral hygiene habits.
- The research highlights the need for improved oral health education and practices to combat high rates of dental caries in these communities.
The geographical distribution of C. botulinum type E and its associated disease, type E botulism in China, is different from that in other areas of the world. Cases of type E botulism generally arise in costal regions.
View Article and Find Full Text PDFSimplified purification method for Clostridium difficile toxin A.
World J Gastroenterol
September 2004
Aim: To establish the purification method for Clostridium difficile (C. difficile) toxin A.
Methods: C.
Development of an ELISA kit using monoclonal antibody to Clostridium difficile toxin A.
World J Gastroenterol
September 2004
Aim: To establish an ELISA kit using monoclonal antibodies against Clostridium difficile (C. difficile) toxin A.
Methods: An indirect sandwich ELISA was described using the purified rabbit monospecific antiserum as capturing antibody.
[Preparation and characterization of monoclonal antibody against Clostridium difficile toxin A].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2004
Article Synopsis
- The study aimed to create monoclonal antibodies (mAbs) against Clostridium difficile toxin A and assess their properties.
- Researchers immunized BALB/c mice with the toxin and fused their immune cells with myeloma cells to generate hybridoma cells, which were then screened for desired mAbs.
- Six mAbs were successfully produced, showing varying immunoglobulin classes, high relative affinities, and specific reactivity to the toxin, although none exhibited neutralization activity, making them suitable for detection purposes.