Discovering a novel glycosyltransferase gene enhances main metabolites production of .

is a filamentous fungus used for both medicinal and culinary purposes. It exhibits a wide range of pharmacological activities due to its valuable contents of cordycepin, polysaccharides, carotenoids, terpenoids and other metabolites. However, strains are highly susceptible to irreversible degradation in agricultural production, which is often manifested as a prolonged color change period and a significant decrease in the production of secondary metabolites.

Elucidation of the Complete Biosynthetic Pathway of Phomoxanthone A and Identification of a Para-Para Selective Phenol Coupling Dimerase.

Fungal cytochrome P450 enzymes have been shown to catalyze regio- and stereoselective oxidative intermolecular phenol coupling. However, an enzyme capable of catalyzing undirected - (C4-4') coupling has not been reported. Here, we revealed the biosynthetic gene cluster (BGC) of phomoxanthone A from the marine fungus sp.

A new monoterpene glycoside from and anticomplementary activity of its compounds.

A new monoterpene glycoside named as pedivertoside D (), together with 13 known compounds (-, resp.) were isolated from the whole plant of L. The new compound was identified as (,,)-5,8-dihydrooxy-2,6-dimethyl-3,7-octadienyl-β-D-glucopyranoside by spectroscopic methods including 2 D-NMR techniques.

Intermolecular C-H Amidation of (Hetero)arenes to Produce Amides through Rhodium-Catalyzed Carbonylation of Nitrene Intermediates.

Amide bond formation is one of the most important reactions in organic chemistry because of the widespread presence of amides in pharmaceuticals and biologically active compounds. Existing methods for amides synthesis are reaching their inherent limits. Described herein is a novel rhodium-catalyzed three-component reaction to synthesize amides from organic azides, carbon monoxide, and (hetero)arenes via nitrene-intermediates and direct C-H functionalization.

A novel dimeric flavonol glycoside from subsp. .

A novel dimeric flavonol glycoside, Cynanflavoside A (), together with six analogues, kaempferol-3---L-rhamnopyranosyl-(1→2)--D-glucopyranoside (), quercetin-3---L-rhamnopyranosyl-(1→2)--D-glucopyranoside (), kaempferol-3---L-rhamnopyranosyl-(1→2)--D-xylopyranoside (), quercetin-3---L-rhamnopyranosyl-(1→2)--D-xylopyranoside , kaempferol-3---D-glucopyranosyl-7---L-rhamnopyranoside (), and quercetin-3--galactoside () were isolated from the -butyl alcohol extract of subsp. . Their structures were determined spectroscopically and compared with previously reported spectral data.

Iridoids from Pedicularis verticillata and Their Anti-Complementary Activity.

Three new iridoids named as pediverticilatasin A - C (1 - 3, resp.), together with five known iridoids (4 - 8, resp.) were isolated from the whole plants of Pedicularis verticillata.

[Effects of Different Modifier Concentrations on Lead-Zinc Tolerance, Subcellular Distribution and Chemical Forms for Four Kinds of Woody Plants].

Four kinds of lead-zinc tolerant woody plants: Nerium oleander, Koelreuteria paniculata, Paulownia and Boehmeria were used as materials to estimate their enrichment and transferable capacity of lead (Pb) and zinc (Zn) and analyze the subcellular distribution and chemical speciation of Zn and Ph in different parts of plants, under different modifier concentrations (CK group: 100% lead-zinc slag plus a small amount of phosphate fertilizer, improved one: 85% of lead-zinc slag ± 10% peat ± 5% bacterial manure plus a small amount of phosphate fertilizer, improved two: 75% lead-zinc slag ± 20% peat ± 5% bacterial manure ± a small amount of phosphate). Results showed that: (1) The content of Pb, Zn in matrix after planting four kinds of plants was lower than before, no significant difference between improved one and improved two of Nerium oleander and Boehmeria was found, but improved two was better than improved one of Paulownia, while improved one was better than improved two of Koelreuteria paniculata; Four plants had relatively low aboveground enrichment coefficient of Pb and Zn, but had a high transfer coefficient, showed that the appropriate modifier concentration was able to improve the Pb and Zn enrichment and transfer ability of plants. (2) In subcellular distribution, most of Pb and Zn were distributed in plant cell wall components and soluble components while the distribution in cell organelles such as mitochondria, chloroplasts and nucleus component were less.