Publications by authors named "Si-ji Nian"
Wei Sheng Wu Xue Bao
August 2007
Article Synopsis
- A new and straightforward PCR method was developed for detecting the TCK pathogen, focusing on amplifying a unique 1322bp DNA fragment.
- Two pairs of specific primers were created: CQUK2/CQUK3 successfully amplified a 747bp target from TCK strains, while CQUK4/CQUK5 produced a 200bp band without false positives from TCT strains.
- The detection system also includes Tilletia genus primers as internal controls to ensure accurate results by identifying PCR inhibitors.
