Zhonghua Kou Qiang Yi Xue Za Zhi
September 2012
Shanghai Kou Qiang Yi Xue
October 2012
Purpose: To evaluate osteogenesis in canine bone marrow mesenchymal stem cells(BMSCs) transduced by point mutant HIF-1α.
Methods: Lenti-WT (wild type HIF-1α), Lenti-MT (mutant type HIF-1α), and Lenti-LacZ (the control group) were constructed with LR recombination system. And then Lenti-LacZ, Lenti-WT and Lenti-MT were used to transduce canine BMSCs.