Quercetin inhibits proliferation and invasion acts by up-regulating miR-146a in human breast cancer cells.

Breast cancer is the most common female malignancies in the world which seriously impacts the female health. In recent years, various studies have been reported to determine the relevance of miRNAs to human cancer. One of these miRNAs, miR-146a has been down-regulated in multiple human cancer types, but up-regulation showed inducing apoptosis.

[Application of polytetrafluoroethylene as VEGF gene carrier].

Objective: To investigate the feasibility of vascular graft material polytetrafluoroethylene (PTFE) as gene carrier of vascular endothelial growth factor (VEGF).

Methods: The PTFE strips were soaked in pCDI-hVEGF(121) solution,marked with EB solution, and then observed under ultraviolet light. The PTFE strips carrying pCDI-hVEGF(121) were soaked in normal saline and the value of optical density at 260 nm on different time was measured, then the controlled release curve was made.

Diagnosis and management of colonic injuries following blunt trauma.

Aim: To retrospectively evaluate the preoperative diagnostic approaches and management of colonic injuries following blunt abdominal trauma.

Methods: A total of 82 patients with colonic injuries caused by blunt trauma between January 1992 and December 2005 were enrolled. Data were collected on clinical presentation, investigations, diagnostic methods, associated injuries, and operative management.

Proliferation of endothelial cell on polytetrafluoroethylene vascular graft materials carried VEGF gene plasmid.

Objective: To investigate whether vascular endothelial growth factor (VEGF) gene plasmid carried by polytetrafluoroethylene (PTFE) vascular graft materials could transfect endothelial cells (ECs) and promote their growth.

Methods: PTFE vascular graft materials carried with pCDI-hVEGF(121), pCDI or pEGFP were incubated in Tris-buffer solution and the values of optical density of 260 nm at different time were plotted, then the DNA controlled release curve was made. ECs derived from human umbilical vein were seeded on the pCDI-hVEGF(121)/pCDI/pEGFP-PTFE materials or tissue culture plates, ECs numbers were counted and VEGF protein concentrations at different time were measured by enzyme-linked immunoadsorbent assay method.

Prognostic significance of vascular endothelial growth factor expression and microvessel density in carcinoma of ampulla of Vater.

Background/aims: To investigate whether the expression of vascular endothelial growth factor (VEGF) and microvessel density (MVD) are of prognostic significance in ampullary carcinoma.

Methodology: Twenty-two resected tumor specimens from patients with ampullary carcinoma were immunohistochemically stained for VEGF and CD34 (surrogate for vessels) by streptavidin-peroxidase method.

Results: Expression of VEGF in tumor tissue was found in 50% of patients.

Patients' quality of life after laparoscopic or open cholecystectomy.

Objective: This study was aimed at evaluating and comparing the quality of life in patients who underwent laparoscopic and open cholecystectomy for chronic cholecystolithiasis.

Methods: The study included 25 patients with laparoscopic cholecystectomy (LC group) and 26 with open cholecystectomy (OC group). The quality of life was measured with the Gastrointestinal Quality of Life Index (GLQI) preoperatively, thereafter regularly at 2, 5, 10 and 16 weeks after the operation.

Surgical management of gastric stump cancer: a report of 37 cases.

Objective: To observe the clinicopathological characteristics of gastric stump cancer (GSC) and evaluate the benefits of radical surgery of GSC.

Methods: The clinicopathological characteristics and postoperative survival time of 37 GSC patients who underwent surgery were investigated retrospectively. The survival time was compared according to the type of surgical operation (radical resection vs palliative operation).

Quality of life in patients with liver cancer after operation: a 2-year follow-up study.

Background: Quality of life (QL) is a concept which reflects the physical, social, and emotional attitudes and behaviours of an individual. QL assessment is becoming increasingly recognised as an outcome and predictor for cancer patients. Although hepatectomy has been widely accepted as treatment of choice to offer a chance of cure for patients with liver cancer, little is known about the subjective clinical results after this operation.

[Dual cell seeding to improve cell retention on polytetrafluoroethylene grafts].

Objective: To improve cell retention on the graft with dual cell seeding technique, adding a layer of smooth muscle cells (SMCs) between the graft and endothelial cells (ECs).

Methods: Polytetrafluoroethylene (PTFE) grafts pretreated with fibronectin were seeded with SMC followed by ECs one day later and exposure to an in vitro flow system. The number of ECs on grafts was counted under microscope.