Discovery of soluble epoxide hydrolase inhibitors from Inula britannica: Inhibition kinetics, molecular dynamics simulation, biochemical, and in vitro cell-based studies.

Soluble epoxide hydrolase (sEH) is an enzyme involved in transforming epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs). EETs play a pivotal role in maintaining anti-inflammatory response and pain regulation, making sEH as an important target for therapeutic interventions. Based on our present study of discovery of sEH inhibitors, herein, six new (1-6) and fifteen known compounds (7-21) with inhibitory effects toward sEH were isolated from Inula britannica.

[Spectrophotometric determination of deoxyribonucleic acid by its quenching effect on acridine orange].

Based on the reaction of acridine orange with deoxyribonucleic acid (DNA), and under the optimized condition, a novel method to determine the DNA was developed with the spectrophotometry. The absorbance of acridine orange at the maximum absorption wavelength of 444 nm responded to the concentration of DNA negatively with excellent linearity. It has an upper linear limit concentration of 8.

[Studies on the interaction between RNA with neutral red and determination of RNA by spectrophotometry].

An analytical method for the determination of ribonucleic acid by spectrophotometry was established. At the maximum absorption wavelength for neutral red in B-R buffer solution, and under the best conditions, the decrease in the absorbance was linear with the amount of ribonucleic acid. The linearity range was 0.

[Studies on the interaction between RNA with methyl violet and determination of RNA by spectrophotometry].

An analytical method for the determination of ribonucleic acid was established by spectrophotometry. At maximum absorption wavelength for methyl violet in the B-R buffer solution, and under the best conditions, the degree of decrease of the absorbance was linear with the amount of ribonucleic Acid. It was a new and preferable approach for the determination of ribonucleic Acids.

[Multi-wavelength negative absorption fading spectrophotometry to determine trace nitrite and it's mechanism].

Using negative absorption rectifyingtechniquie, the authors investigated spectra of the reaction of nitrite with acridine yellow in 1.0 mol x dm(-3) (1 dm = 10 cm = 0.1 m) hydrochloric medium.