Non-uniform image reconstruction for fast photoacoustic microscopy of histology imaging.

Photoacoustic microscopic imaging utilizes the characteristic optical absorption properties of pigmented materials in tissues to enable label-free observation of fine morphological and structural features. Since DNA/RNA can strongly absorb ultraviolet light, ultraviolet photoacoustic microscopy can highlight the cell nucleus without complicated sample preparations such as staining, which is comparable to the standard pathological images. Further improvements in the imaging acquisition speed are critical to advancing the clinical translation of photoacoustic histology imaging technology.

Effectiveness of a case management model in newly treated smear-positive pulmonary tuberculosis patients.

Introduction: To investigate the effectiveness of the case management mode on the application of smear-positive pulmonary tuberculosis patients.

Methodology: This was a randomized control trial. A total of 70 newly diagnosed smear-positive pulmonary tuberculosis patients were recruited and been randomly divided into experimental group and control group, with 35 participants in each group.

microRNA-329 suppresses epithelial-to-mesenchymal transition and lymph node metastasis in bile duct cancer by inhibiting laminin subunit beta 3.

Bile duct cancer (BDC), also known as cholangiocarcinoma, is a highly desmoplastic cancer with a growth pattern characterized by periductal extension and infiltration. Studies have suggested that microRNAs (miRNAs) play an important role in BDC progression. Here we aim at investigating the effects of miR-329 on BDC development, focusing especially on epithelial-to-mesenchymal transition (EMT) in vitro and lymph node metastasis in vivo.

Integration of Antibody Array Technology into Drug Discovery and Development.

Antibody arrays represent a high-throughput technique that enables the parallel detection of multiple proteins with minimal sample volume requirements. In recent years, antibody arrays have been widely used to identify new biomarkers for disease diagnosis or prognosis. Moreover, many academic research laboratories and commercial biotechnology companies are starting to apply antibody arrays in the field of drug discovery.

Lamprey: a model for vertebrate evolutionary research.

Lampreys belong to the superclass Cyclostomata and represent the most ancient group of vertebrates. Existing for over 360 million years, they are known as living fossils due to their many evolutionally conserved features. They are not only a keystone species for studying the origin and evolution of vertebrates, but also one of the best models for researching vertebrate embryonic development and organ differentiation.

Application of multiplex immunoassay technology to investigations of ocular disease.

Eye-derived fluids, including tears, aqueous humour and vitreous humour often contain molecular signatures of ocular disease states. These signatures can be composed of cytokines, chemokines, growth factors, proteases and soluble receptors. However, the small quantities (<10 µl) of these fluids severely limit the detection of these proteins by traditional enzyme-linked immunosorbent assay or Western blot.

Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing.

A wealth of research has been available discussing nanogap devices for detecting very small quantities of biomolecules by observing their electrical behavior generally performed in dry conditions. We report that a gold nanogapped electrode with tunable gap length for ultra-sensitive detection of streptavidin based on electrochemical impedance technique. The gold nanogap is fabricated using simple monolayer film deposition and in-situ growth of gold nanoparticles in a traditional interdigitated array (IDA) microelectrode.

Deciphering Asthma Biomarkers with Protein Profiling Technology.

Asthma is a chronic inflammatory disease of the airways, resulting in bronchial hyperresponsiveness with every allergen exposure. It is now clear that asthma is not a single disease, but rather a multifaceted syndrome that results from a variety of biologic mechanisms. Asthma is further problematic given that the disease consists of many variants, each with its own etiologic and pathophysiologic factors, including different cellular responses and inflammatory phenotypes.

Selective detection toward quercetin and kaempferol on NH3-plasma treated carbon nanotubes modified glassy carbon electrode.

NH3-plasma treated multi-walled carbon nanotubes (pn-MWCNTs) were prepared based on the plasma technique and developed as sensing materials for detection of quercetin and kaempferol with the differential pulse voltammetry (DPV) and amperometric measurement. Such experimental parameters as pH values, accumulation potential and accumulation time were carefully investigated. The pn-MWCNTs modified electrode (pn-MWCNTs/GCE) was further explored for the analysis of quercetin and kaempferol in diluted blood serum and average recovery rates of 96.

[Mechanism of Wnt signaling pathway regulation by a truncated mutant of Axin2 in colorectal cancer].

Background & Objective: Axin2 gene which negatively regulates the Wnt signaling pathway was cloned recently. A truncated mutation of Axin2 (mtAxin2) is the most common mutation pattern in colorectal cancer (CRC) and could enhance the luciferase activity of T-cell factor (TCF). This study was to explore the mechanism of Wnt signaling pathway regulation by mtAxin2 in CRC.

[Clinical research of taxotere in treatment of metastatic breast cancer].

Background & Objective: Recently, high-dose docetaxel has been proved to be an effective antineoplastic drug in the treatment of metastatic breast cancer.However,there was a few reports about moderate-dose docetaxel and Adriamycin in the treatment of metastatic breast cancer. This study was designed to observe the response rate and toxic reaction of Taxotere and Taxotere plus Adriamycin in the treatment of metastatic breast cancer.