[In vitro biological evaluation of PEGylated poly(glycerol sebacate)/β-TCP-coating modified magnesium alloy].

Purpose: To prepare PEGS/β-TCP modified magnesium alloy (PEGS/β-TCP/MZG) membranes by forming a glycolated poly(sebacate)/β-tricalcium phosphate (PEGS/β-TCP) coating on the surface of magnesium-zinc-gadolinium alloy (MZG) membranes, and to evaluate the osteogenic induction activity and immunomodulatory properties of PEGS/β-TCP/MZG using the material extract medium.

Methods: PEGS/β-TCP coating was prepared on the surface of MZG by solvent method, and the PEGS/β-TCP/MZG membrane was fabricated and compared with PEGS/β-TCP and MZG to examine the morphology, composition, and hydrophilicity. The amount of magnesium ions released and the pH value of the materials were tested after 3 days of immersion.

[Clinicopathological features of children with lupus nephritis with positive antineutrophil cytoplasmic antibody].

Objective: To study the clinicopathological features of children with lupus nephritis (LN) with positive anti-neutrophil cytoplasmic antibody (ANCA).

Methods: A retrospective analysis was performed for the children who were diagnosed with LN in the First Affiliated Hospital of Sun Yat-sen University from January 2003 to December 2019. According to the results of serum ANCA, they were divided into two groups: ANCA-positive group (=59) and ANCAnegative group (=454).

[The effect of Foxc2 overexpression on the osteogenic properties of C3H10T1/2 cells].

Purpose: To investigate the effect of Foxc2 overexpression on osteogenic and adipogenic differentiation of C3H10T1/2 cells.

Methods: C3H10T1/2 cells were transfected with plenti-Foxc2 and selected with puromycin for stable clones. The expression of Foxc2 was determined by real-time PCR and Western blot.

[Study of the influence of nodal liquefaction in measurement of the ADC value of OSCC metastatic lymph nodes in patients with oral squamous cell carcinoma].

Purpose: To study the influence of nodal liquefaction portion in the measurement of the ADC value of metastatic lymph nodes in patients with oral squamous cell carcinoma (OSCC) on DW-MRI images.

Methods: According to the postoperative histopathological examination results, the DW-MRI data of 25 OSCC patients was analyzed. The ADC values of both solid portions of metastatic lymph nodes and the whole metastatic lymph nodes with internal liquefaction were selected and measured.

Perinatal stem cells: A promising cell resource for tissue engineering of craniofacial bone.

In facing the mounting clinical challenge and suboptimal techniques of craniofacial bone defects resulting from various conditions, such as congenital malformations, osteomyelitis, trauma and tumor resection, the ongoing research of regenerative medicine using stem cells and concurrent advancement in biotechnology have shifted the focus from surgical reconstruction to a novel stem cell-based tissue engineering strategy for customized and functional craniofacial bone regeneration. Given the unique ontogenetical and cell biological properties of perinatal stem cells, emerging evidence has suggested these extraembryonic tissue-derived stem cells to be a promising cell source for extensive use in regenerative medicine and tissue engineering. In this review, we summarize the current achievements and obstacles in stem cell-based craniofacial bone regeneration and subsequently we address the characteristics of various types of perinatal stem cells and their novel application in tissue engineering of craniofacial bone.