EVALUATION OF THE COMPLEX USE OF PLANT TEST-SYSTEMS TO DETERMINE THE NATURE AND INTENSITY OF THE MUTAGENIC BACKGROUND OF THE ENVIRONMENT.

Objective: Aim: A comprehensive evaluation of the effectiveness of the use of plant test-systems as biological objects for the identification of the specifitown of the effect of mutagens on the hereditary apparatus.

Patients And Methods: Materials and methods: With the use of test-systems (determination of the gonadotoxitown of plant pollen grains, the Allium cepa-test and the use of celandine greater Chelidonium majus as an accumulative phytoindicator) was analyzed the ecological situation of the areas in Ivano-Frankivsk region. The samples of soil, water, generative and vegetative organs of plants were taken in the territories of the Carpathian region and tested in the following areas: ecologically safe, conditionally ecologically safe and ecologically dangerous areas.

characterization of lepr gene Q223R (RS1137101) genotypes in patients with knee joint osteoarthritis of different radiographic stages.

Objective: Introduction: Osteoarthritis is a multifactorial joint disease with a significant role of the genetic factor. The numerous studies have demonstrated that genetic dependence is specific for individual hand, hip and knee regions with a genetic contribution to the pathogenesis of osteoarthrosis varying from 40% to 65%. To assess the role of leptin gene receptor functional activity disturbance in the pathogenesis of osteoarthrosis, it is important to study the relationship between the LEPR gene polymorphism and a number of clinical and laboratory parameters.

Haloperidol administered subchronically reduces the alcohol-deprivation effect in mice.

During the pre-experimental phase, hybrid (CBA x C57BL) male mice having had 16 weeks free access to food, water and flavored 30% alcohol were deprived of alcohol for 3 days. The next day they were given free choice between similarly flavored water and 30% alcohol. The mice were divided into two subgroups having (HD) or lacking (LD) the deprivation-induced elevation in alcohol intake during the first 1.

Explorative and drinking behavior after prolonged access to alcohol and following chronic piracetam administration in mice.

Male mice with free access to food, water, and alcohol for 44 weeks were deprived of alcohol for 3 days and then segregated into two groups having (HD) and lacking (LD) the alcohol deprivation-induced elevation in intake. On week 47, the HD and LD groups (divided into subgroups matched for drinking) received either vehicle or piracetam (400 mg/kg) for 10 days. On the last treatment day, alcohol was again withheld.

Effect of chronic piracetam on age-related changes of cross-maze exploration in mice.

Normal aging is known to deteriorate memory, spatial orientation, and perceptual recognition. Experiment 1 examined behavioral manifestations of aging by using a cross-maze exploration test in 2-, 6-, and 10-month-old hybrid mice (CBA x C57BL). A decrease in explorative patrolling and an increase in arm reentries, a latency to start and a total time of exploration were found in 10-month-old mice.

Genetic control of alcohol deprivation effect in congenic mice.

Male mice of A(4R) and AKM congenic resistant strains, differing in E alpha and D loci of H-2 major histocompatibility complex (MHC) only, were tested for cross-maze exploration and for behavior in unavoidable slip funnel situation. On the following 15 weeks the mice had free access to food, water, and 30% alcohol. On weeks 16-17 the individual alcohol and water intakes were measured before and after 3 days of alcohol deprivation to evaluate the alcohol deprivation effect (ADE), which is known to depend upon both genetic and environmental factors.