[CHARACTERISTIC OF APOPTOSIS OF CORONARY ARTERY CELLS WITH ATHEROSCLEROTIC LESIONS].

Aim: To study apoptosis of endothelial cells (EC), macrophages (MF) and smooth muscle cells (SMC) in the early atherosclerotic process (prior to plaque formation), elucidate mechanisms of its realization and evaluate effect on progression of atherosclerosis.

Materials And Methods: Histopathological studies were performed on coronary arteries affected by atherosclerosis taken at autopsy of patients with coronary heart disease (n = 63). To detect apoptosis, the TUNEL method was used for calculating the apoptotic index (AI) in paraffin sections.

[Micromorphology of decayed tooth tissues covered with artificial crown].

Pathomorphological changes in dentine covered with artificial crown were studied in 40 decalcified teeth extracted from patients aged 35-61 years. The study of the decayed dentin microscopic structure revealed the uniform mineralization of dentinal tubules in 20% of cases, while in the absence of visually apparent caries it was present in 80% of cases. Irregular dentin structure was seen in 45% of caries cases and in 30% of observations with no signs of caries.

[Apoptosis cells of coronary artery wall as developing and progressing factor of coronary sclerosis].

Objective: To study apoptosis of individual cellular components of the vascular wall of coronary arteries at different morphological stages of atherosclerosis.

Material And Methods: The study was performed on coronary arteries taken from 52 deceased patients with atherosclerosis and coronary heart disease at different stages of atherogenesis. For morphological study prepared paraffin sections, which were stained for morphological studies were prepared paraffin sections, which were stained with hematoxylin and eosin, by Van Gieson, Masson, on lipids with Sudan black B, according to Van Cossu.

[Influence of hemicellulose based anticoagulant on male rat reproductive function].

Results of estimation of the effect of a new hemicellulose-based anticoagulant drug on the reproductive function of white male rats are presented. No negative impact on the fertility and breed was observed for the drug administered in doses of 5 and 25 mg/kg. However, in a dose of 50 mg/kg, the new drug negatively affected spermatogenesis and decrease the reproductive function of male rats.

[Cultured skin cells interaction with polylactide surface coated by different collagen structures].

The purpose of this work was an optimization of polylactide film surfaces designed for human keratinocytes cultivation. The polylactide films were coated by collagen 1. The experiments showed that uniform covering of polymer surface by collagen, and formation of different collagen structures depend on the mode of the protein application.

[Subacute and primary-chronic osteomyelitis in children].

Clinical specificity and biopsy material from bone foci were studied in 81 children aged from 4 months to 15 years with subacute and primary chronic hematogenic osteomyelitis (PCO). The poorly manifesting course of this pathology is stressed. It is expedient to take into account the morphological alterations in the pathological foci and clinical stages of the disease in diagnosing strategy of treatment of subacute and PCO.

[Elaboration of biodegradable polymer substrate for cultivation of human dermal fibroblasts].

The influence of polylactic acid (PLA) surface films on the pattern of cell behavior was studied. The human dermal fibroblasts were cultivated on PLA covered glasses. The hydrophobic nature of PLA films depends on the availability of polymer solvent in the film preparation.

[The nature of posttraumatic regeneration of the mandible depending on the method of osteosynthesis].

Osteosynthesis of mandibular fragments by the traditional and biomechanics-based methods was studied in dogs. The morphological picture was virtually the same in the immediate periods (5-10 days), whereas in remote periods (1, 3, and 6 months) osteogenesis was more adequate in the group where biomechanically based methods were used, particularly so with the supraosseous miniplates.

[Aplasia of the radius and mechanisms of deformity of the arm].

A complex study (roentgenographic, arteriographic, anatomic, anthrometric and microscopic) of 16 upper extremities of stillborn and deceased infants with aplasia and hypoplasia of the radius has been carried out. The etiologic factors of the defect were chromosomal and monogenic mutations and multifactor causes. It has been demonstrated that: 1) in cases of reduction of the radius the structure of the arteries, nerves and muscles in destroyed mostly in the radial border of the forearm and hand; 2) the severity of the changes in the anatomic depends on the type of the defect; 3) the pathomorphologic manifestations of the forms of the defect characterized by the same severity are mostly similar.

Campomelic syndrome: concepts of the bowing and shortening in the lower limbs.

A comprehensive study (bone roentgenography, arteriography, gross dissection, microscopy of the long bones, and biochemical study of proteoglycan-aggregates in the hyaline cartilage) of the lower limbs in a full-term stillborn with the campomelic syndrome was performed. Hyaline cartilage immaturity of the long bones, dysplasia of growth plates, focal shaft dysplasia, and a defective length of the posterior femur and crus muscles were revealed. The genesis of the bowing and shortening of the long bones in the lower limbs is discussed.

[Hereditary factors and their causative role in anatomic variation].

As demonstrate the literature data and the authors' observations on the composition of the anatomical structures of the extremities at the popliteal pterigyum syndrome of Smith-Lemley-Optis, as well as at some other monogenic syndromes, the manifestation of the anatomical changeability in humans is defined, to an essential degree, by hereditary factors. A suggestion is made that investigation of the anatomical changeability in connection with genetic peculiarities of the organism makes it possible to approach the causal interpretation of the variants and the developmental anomalies and comprehend the sources of multiplicity of forms and structure of the human organs and systems.

Partial trisomy 5q and partial monosomy 5q within the same family.

The observation of partial trisomy for 5q31-5qter and partial monosomy for the same segment in two offspring within the same family is presented. Their normal mother was a balanced carrier of a reciprocal translocation 46,XX,t(5;10) (q31.3;q26).

Elaboration of the phenotypic changes of the upper limbs in the Neu-Laxova syndrome.

We present two new cases and data from a detailed study of the upper limbs (structural changes, the state of peripheral nerves, arteries, muscles and bones) of three stillborn infants with the Neu-Laxova syndrome. The morphogenesis of many of the examined anatomical structures in these fetuses was anomalous. The origin of these anomalies is discussed.

[Pathological anatomy of monogenic syndromes of multiple congenital developmental defects].

Morphological study, with no selection, is performed of 1106 stillborns and children died before one year of age with congenital malformations (CM), 536 (46.9 +/- 1.5%) among them having multiple CM.

The Smith-Lemli-Opitz syndrome. A detailed pathological study as a clue to a etiological heterogeneity.

An analysis of 33 autopsied cases with the Smith-Lemli-Opitz syndrome (including 8 cases from our practice) is presented. Polydactyly in dead SLOS children was found in 51% (17/33) of cases and occurred significantly more often in this group than in the whole group of SLOS (20-22%). Certain morphological differences in the type of renal, cerebral, pulmonary and pancreatic anomalies indicate the existence of two phenotypically similar SLOS: 1) with polydactyly; 2) without it.

Difficulties in classification of the short rib-polydactyly syndromes.

A case of "short rib-polydactyly" (SRP) syndrome (type II-Saldino-Noonan) with a detailed study of the bones, muscles and peripheral nerves in the extremities is presented. Analysis of the literature (35 cases with different forms of SRP) and the comparison of clinical, genetic and morphological data lead us to consider that different types of SRP may be related to different alleles of the same gene. Genetic heterogeneity (mutations of different genes) is also possible.

Partial trisomy 10p in two generations.

Two cases of partial 10p trisomy due to a t(10;20)(p12;p12) in two generations of a family are presented. Analysis of 20 known cases of such aberrations confirmed the opinion of Schleiermacher et al. (1974) that partial trisomy 10p is a distinct clinically recognizable entity.