Draft genome sequence of glyphosate-degrading sp. strain GPK 3 (VKM B-2554D) isolated from agricultural soil.

The genome of sp. isolated from agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 5.

Glyphosate-Induced Phosphonatase Operons in Soil Bacteria of the Genus .

and , bacterial degraders of the herbicide glyphosate, were found to induce phosphonatase (phosphonoacetaldehyde hydrolase, EC 3.11.1.

Characterization of a New Strain Ch2, a Degrader of Toxic Anthropogenic Compounds -Caprolactam and Glyphosate.

In this work, a new Ch2 strain was isolated from soils polluted by agrochemical production wastes. This strain has a unique ability to utilize toxic synthetic compounds such as -caprolactam (CAP) as a sole carbon and energy source and the herbicide glyphosate (GP) as a sole source of phosphorus. Analysis of the nucleotide sequence of the 16S rRNA gene of Ch2 revealed that the strain belongs to the species .

Multifunctional Enzyme with Endoglucanase and Alginase/Glucuronan Lyase Activities from Bacterium Cellulophaga lytica.

Cellulophaga lytica is a Gram-negative aerobic bacterium in the genome of which there are many genes encoding polysaccharide degrading enzymes. One of the enzymes named ClGP contains a glycoside hydrolase domain from the GH5 family and a polysaccharide lyase domain from the PL31 family. The enzyme also contains the TAT signaling peptide and the TIGR04183 domain that indicates extracellular nature of the enzyme.

Draft Genome Sequence of Glyphosate-Degrading Achromobacter insolitus Strain Kg 19 (VKM B-3295), Isolated from Agricultural Soil.

The genome of an strain isolated from an agricultural soil polluted with the herbicide glyphosate is reported. The genome size is 6.4 Mb, with an average G+C content of 65.

Organophosphonates utilization by soil strains of Ochrobactrum anthropi and Achromobacter sp.

Four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. All studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. Only strains Ochrobactrum anthropi GPK 3 and Achromobacter sp.

Glyphosate acetylation as a specific trait of Achromobacter sp. Kg 16 physiology.

The growth parameters of Achromobacter sp. Kg 16 (VKM B-2534 D), such as biomass and maximum specific growth rate, depended only on the source of phosphorus in the medium, but not on the carbon source or the presence of growth factors. With glyphosate as a sole phosphorus source, they were still 40-50 % lower than in media supplemented with orthophosphate or other organophosphonate-methylphosphonic acid.

[Microbial degradation of glyphosate herbicides (review)].

This review analyzes the issues associated with biodegradation of glyphosate (N-(phosphonomethyl)glycine), one of the most widespread herbicides. Glyphosate can accumulate in natural environments and can be toxic not only for plants but also for animals and bacteria. Microbial transformation and mineralization ofglyphosate, as the only means of its rapid degradation, are discussed in detail.

Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp.

Bacterial strains capable of utilizing methylphosphonic acid (MP) or glyphosate (GP) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. The strains isolated from MP-contaminated soils grew on MP and failed to grow on GP. One group of the isolates from GP-contaminated soils grew only on MP, while the other one grew on MP and GP.

Bioremediation of glyphosate-contaminated soils.

Based on the results of laboratory and field experiments, we performed a comprehensive assessment of the bioremediation efficiency of glyphosate-contaminated soddy-podzol soil. The selected bacterial strains Achromobacter sp. Kg 16 (VKM B-2534D) and Ochrobactrum anthropi GPK 3 (VKM B-2554D) were used for the aerobic degradation of glyphosate.

[Sorption and microbial degradation of glyphosphate in soil suspensions].

Sorption and microbial destruction of glyphosphate, the active agent of the herbicide Groundbio, in suspensions of sod-podzolic and gray forest soils has been studied. According to the values of the adsorptive capacity (3560 and 8200 mg/kg, respectively) and the Freundlich constants (Kf, 15.6 and 18.

Glyphosate bioavailability in soil.

Biodegradation of glyphosate in sod-podzol soil by both the indigenous micro flora and the introduced strain Ochrobactrum anthropi GPK 3 was studied with respect to its sorption and mobility. The experiments were carried out in columns simulating the vertical soil profile. Soil samples studied were taken from soil horizons 0-10, 10-20, and 20-30 cm deep.

[Microbial degradation of organophosphonates by soil bacteria].

Bacteria that can utilize glyphosate (GP) or methylphosphonic acid (MPA) as a sole phosphorus source have been isolated from soil samples polluted with organophosphonates (OP). No matter which of these compounds was predominant in the native habitat of the strains, all of them utilized methylphosphonate. Some of the strains isolated from GP-polluted soil could utilize both phosphorus sources.

[Methodical approaches to evaluation of the safety of veterinary preparation application].

Evaluation of toxicologic hazards associated with the use of veterinary preparations against ecto- and endoparasites of animals and poultry with the purpose of prophylaxis and treatment, evaluation of industrial safety and determination of the upper limits of their residue in live-stock products and the environment, underlies protection of population's and operators' health from the effects of the drugs. The article shows the difference between the acute toxicity parameters of cypermethrin isomers and demonstrates that they enter the organism chiefly through the skin.

[Hygienic problems of population health maintenance under the extremal conditions of North].

A range of multi-sided studies, carried out in various regions of Far North (Jamalo-Nenets autonomous region, Komi republic, Sakha republic (Yakutia)) have established the leading hygienic risk factors of population health deterioration, including those characteristic of small nationalities of North. The consideration of the obtained data, including regional alimentary status peculiarities, the condition of metabolic processes and antioxidant balance of the organism, allowed establishment of effective approaches to the maintenance of notherners' health. These approaches are based upon the optimization of nutrition as an element of population health control under extremal conditions.

[Impact of the climate of the Extreme North and education conditions on young school children].

The scientifically grounded sound regimen is required for schoolchildren, younger ones in particular, who most experience unfavourable environmental factors. Particular emphasis should be laid on the regulation of educational burden at primary school since the irrational distribution of classes leads to the development of fatigue mainly in young schoolchildren and hence to lower working capacities and poor comprehensibility. The investigations have supported the earlier data that the children show the strained body's functional status which is deteriorated by the influence of educational burden.

[Psychoemotional status of primary schoolchildren in the Far North].

Social and natural factors, environmental pollution and general school reforms create prerequisites for deterioration of children's mental health in the Arctic. Therefore, it is necessary to modify an educational process, especially to transfer classes to later hours than those in the temperate zone of the country.

[Problems in the hygienic rationalization of the therapeutic and prophylactic nutrition of industrial workers].

Based on generalization and analysis of instructions and guidelines for therapeutical and prophylactic diets of workers, ways of its optimization were proposed, by using bifide-containing acid dairy products as a preventive agent against possible occupational diseases caused by occupational factors.

[Physiologic and hygienic bases of the rationalization of work schedules of several groups of miners in Arctic regions].

The article presents some data describing functional state in tunnelers and mining operators. That information should be considered in setting the rational schemes of work and rest during shifts in Transpolar conditions. The authors justified that heart rate is an expedient criterion of functional strain and useful for calculating the necessary rest time.

[The experimental morphofunctional changes in the body under the influence of dry environmental air].

Analysis of morphological changes in organs, tissues and cells of animal organisms (white rats) is presented in interrelations with shifts in somatometric parameters, fluctuations in water exchange induced by the decreased content of diluted liquid vapours in the air. On the basis of studies results the leading role of low humidity of the environment in the development of changes of the dehydration syndrome type has been proved.

[Thermal state of women working outdoors under conditions of Far North].

New data on the thermal state of women working outdoors in the region beyond the polar circle are presented. A number of characteristics of female body heat exchange and their thermal state which should be taken into account when developing the cold-protective clothes have been determined.