[Callus induction, subculture, and browning inhibition of the anticancer plant ].

In order to obtain more effective cell culture parameters of Taxus anticancer plants, we optimized the callus induction and subculture conditions of the explants (stem segments with buds) of the anticancer medicinal plant by using the plant tissue culture technology and orthogonal test. Furthermore, we studied the method to inhibit browning in the culture. The results indicated that the optimal conditions for inducing callus was culture in the medium composed of B+0.

[Pollution Characteristics and Sources of Water-soluble Ions in PM in Zhengzhou City].

In order to explore the pollution characteristics, seasonal variations, and sources of water-soluble inorganic ions (WSIIs) in PM in Zhengzhou, PM samples were seasonally collected from December 2020 to October 2021; then, combining gaseous pollutants (SO, NO, and O) and meteorological parameters (temperature and relative humidity), nine WSIIs (NO, NH, SO, Ca, K, Na, Mg, F, and Cl) were analyzed. The results showed that the annual average concentration of the total water-soluble ions (TWSIIs) was (39.34±21.

Technical solutions for minimizing wheat grain cadmium: A field study in North China.

The minimization of Cd pollution in wheat is urgently needed in many parts of the world. Thus, the aims of the present study were to evaluate the feasibility of popular technologies (i.e.

Farmland heavy metals can migrate to deep soil at a regional scale: A case study on a wastewater-irrigated area in China.

Heavy metal risks to human health in farmland of wastewater-irrigated areas have long been recognized. It remains to be shown whether farmland heavy metals from wastewater irrigation can migrate to deeper soil at a regional scale. In this study, nine soil cores deep to 30 m from three transects (A, B and C) of a linear wastewater reservoir and the adjacent farmland topsoils and wheat grains were sampled.

[Air Pollution Characteristics and Quantitative Evaluation of Multi-scale Transport in the Beijing-Tianjin-Hebei Region in January, 2016].

Based on atmospheric monitoring data and the WRF-CAMx model, this study analyzed the characteristics of air pollution and performed a quantitative assessment of PM cross-border transport in the Beijing-Tianjin-Hebei (BTH) region in January 2016. The results showed that the average concentrations of PM, PM, SO, NO, and CO were 89.5 μg·m, 135.

Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants.

Drought is a major natural disaster that seriously affects agricultural production, especially for winter wheat in boreal China. As functional proteins, the functions and mechanisms of glyceraldehyde-3-phosphate dehydrogenase in cytoplasm (GAPCs) have remained little investigated in wheat subjected to adverse environmental conditions. In this study, we cloned and characterized a GAPC isoform in wheat.

Correction: Xixi, Li., et al. The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat. 2019, , 1104.

The author wishes to make the following correction to this paper [...

The specific W-boxes of GAPC5 promoter bound by TaWRKY are involved in drought stress response in wheat.

Drought is one of the most common abiotic stresses, and can limit wheat yield, crops and productivity. GAPCs play vital roles under drought stress conditions in multiple species. The aim of this experiment was to determine the regulatory mechanism of TaGAPC5 under drought stress.

Iron solubility in fine particles associated with secondary acidic aerosols in east China.

Soluble iron (Fe) in aerosols contributes to free oxygen radical generation with implications for human health, and potentially catalyzes sulfur dioxide oxidation. It is also an important external source of micronutrients for ocean ecosystems. However, factors controlling Fe concentration and its contribution to total iron (Fe) in aerosols remain poorly understand.

Cytosolic glyceraldehyde-3-phosphate dehydrogenase 2/5/6 increase drought tolerance via stomatal movement and reactive oxygen species scavenging in wheat.

Drought is a major threat to wheat growth and crop productivity. However, there has been only limited success in developing drought-hardy cultivars. This lack of progress is due, at least in part, to a lack of understanding of the molecular mechanisms of drought tolerance in wheat.

SmbHLH3 acts as a transcription repressor for both phenolic acids and tanshinone biosynthesis in Salvia miltiorrhiza hairy roots.

Phenolic acids and tanshinones are the two groups of pharmaceutically active metabolites in Salvia miltiorrhiza Bunge. Their contents are the key quality indicator to evaluate S. miltiorrhiza.

TaWRKY40 transcription factor positively regulate the expression of TaGAPC1 to enhance drought tolerance.

Backgrounds: Drought stress is one of the major factors that affects wheat yield. Glyceraldehyde-3-Phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that plays the important role in abiotic stress and plant development. However, in wheat, limited information about drought-responsive GAPC genes has been reported, and the mechanism underlying the regulation of the GAPC protein is unknown.

The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat.

Background: Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences.

The Plastidial Glyceraldehyde-3-Phosphate Dehydrogenase Is Critical for Abiotic Stress Response in Wheat.

Plastidial glyceraldehyde-3-phosphate dehydrogenase (GAPDH, GAPCp) are ubiquitous proteins that play pivotal roles in plant metabolism and are involved in stress response. However, the mechanism of GAPCp's function in plant stress resistance process remains unclear. Here we isolated, identified, and characterized the gene from Chinese Spring wheat for further investigation.

Climatic factors control the geospatial distribution of active ingredients in Salvia miltiorrhiza Bunge in China.

Climate change profoundly influences the geospatial distribution of secondary metabolites and causes the geographical migration of plants. We planted seedlings of the same species in eighteen ecological regions along a latitudinal gradient in eastern and western China, in order to explore the regulation of multi-climatic factors on active ingredient accumulation in Salvia miltiorrhiza Bunge. The correlations between six active ingredient contents and ten climatic factors were investigated to clarify their relationships.

[Identification and characterization of a taxol-producing endophytic fungus from Taxus media].

To enrich the resource pool of endophytic fungi from plants which produce taxol, a taxol-producing endophytic fungus TMS-26 was isolated from the stem of Taxus Media. The result of high performance liquid chromatography (HPLC) showed that TMS-26 extract exhibited similar chromatographic peaks and retention time (4.545 min) with authentic taxol.

Isolation and molecular identification of endophytic diazotrophs from seeds and stems of three cereal crops.

Ten strains of endophytic diazotroph were isolated and identified from the plants collected from three different agricultural crop species, wheat, rice and maize, using the nitrogen-free selective isolation conditions. The nitrogen-fixing ability of endophytic diazotroph was verified by the nifH-PCR assay that showed positive nitrogen fixation ability. These identified strains were classified by 879F-RAPD and 16S rRNA sequence analysis.

Comparison among soil additives for enhancing Pteris vittata L.: Phytoremediation of As-contaminated soil.

Pot experiments were conducted to assess the effects of monoammonium phosphate (NHHPO) and citric acid (CA) on the arsenic uptake of Chinese brake fern (Pteris vittata L. in two typical arsenic-contaminated soils i.e.

Expression analysis and promoter methylation under osmotic and salinity stress of TaGAPC1 in wheat (Triticum aestivum L).

Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) catalyzes a key reaction in glycolysis and encoded by a multi-gene family which showed instability expression under abiotic stress. DNA methylation is an epigenetic modification that plays an important role in gene regulation in response to abiotic stress. The comprehension of DNA methylation at promoter region of TaGAPC1 can provide insights into the transcription regulation mechanisms of plant genes under abiotic stress.

Identification and Characterization of Salvia miltiorrhizain miRNAs in Response to Replanting Disease.

Replanting disease is a major factor limiting the artificial cultivation of the traditional Chinese medicinal herb Salvia miltiorrhiza. At present, little information is available regarding the role of miRNAs in response to replanting disease. In this study, two small RNA libraries obtained from first-year (FPR) and second-year plant (SPR) roots were subjected to a high-throughput sequencing method.

PM10 mass concentration, chemical composition, and sources in the typical coal-dominated industrial city of Pingdingshan, China.

The atmospheric pollution created by coal-dominated industrial cities in China cannot be neglected. This study focuses on the atmospheric PM10 in the typical industrial city of Pingdingshan City in North China. A total of 44 PM10 samples were collected from three different sites (power plant, mining area, and roadside) in Pingdingshan City during the winter of 2013, and were analyzed gravimetrically and chemically.

Genome-wide identification and characterization of Glyceraldehyde-3-phosphate dehydrogenase genes family in wheat (Triticum aestivum).

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a central enzyme in glycolysi, we performed genome-wide identification of GAPDH genes in wheat and analyzed their structural characteristics and expression patterns under abiotic stress in wheat.

Results: A total of 22 GAPDH genes were identified in wheat cv. Chinese spring; the phylogenetic and structure analysis showed that these GAPDH genes could be divided into four distinct subfamilies.