Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides.

Determination of aflatoxin B1 (AFB1) is still a big issue in food safety. In this paper, we developed a luminescence AFB1 detection method combined with ATP-releasing nucleotides (ARNs) and AFB1 aptamer. Firstly, using a new coupling method, we synthesized two ARNs (dTPA and dGPA) in a yield of 67% and 58%, respectively.