RNA-Seq and Iso-Seq Reveal the Important Role of and Genes in Accumulation of Scopoletin in Noni ().

Scopoletin, the main component of clinical drugs and the functional component of health products, is highly abundant in noni fruit (). Multiple enzyme genes regulate scopoletin accumulation. In the present study, differentially expressed genes of noni were analyzed by RNA sequencing (RNA-Seq) and the full-length genes by isoform-sequencing (Iso-Seq) to find the critical genes in the scopoletin accumulation mechanism pathway.

Processing of Noni Liquor based on response surface methodology.

Noni ( L.) is widely used as a health food and medicine because it is rich in polysaccharides, polyphenols, and flavonoids; it is precisely because noni is rich in these substances that people usually like to soak it in liquor to drink. This study sought to maximize the main active constituents (polysaccharides, polyphenols, and flavonoid s) dissolved in liquor and spirits soaked with noni fruit, using ethanol as the solvent to optimize the extraction conditions using response surface methodology.