Zh Mikrobiol Epidemiol Immunobiol
April 1992
Study of different methods of hemosensitization helped detect the optimum technology for the preparation of parainfluenza (type 3) antigenic erythrocyte diagnosticum. This technology ensures the highest sensitivity, specificity, economy (with respect to viral antigen consumption) and stability of the reagent. Titration of antibodies by means of the new diagnosticum and the detection of parainfluenza cases among children with acute respiratory virus infections, acute and chronic glomerulonephritis have been highly effective, considerably more effective than similar determinations by the hemagglutination inhibition test.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
September 1991
Enzyme immunoassay (EIA) with the use of test systems based on recombinant HIV antigens has yielded positive results much more often than that with the test systems based on HIV lysate. In comparison with the use of protein A-peroxidase conjugate, the use of antiglobulin conjugates decreased the specificity of EIA, thus ruling out the false positive results. The tested sera containing antibodies to antigens of enteric bacilli could yield false positive EIA results with test systems prepared from recombinant antigens due to the interaction of these antibodies with antigens of enteric bacilli.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
December 1985
The possibility of detecting M. pneumoniae antigen and antibodies to it, incorporated into immune complexes, in the sera of patients with acute pneumonia by means of erythrocyte diagnosticums was studied, and the immunological characterization of these complexes was made. In patients with mycoplasmal pneumonia M.
View Article and Find Full Text PDFAntibiot Med Biotekhnol
March 1985
A simple method for quantitative estimation of the antiproliferative activity of human interferon is described. The method is based on direct visual estimation (with the use of an inverted microscope) of various doses of interferon with respect to proliferation and colony formation of human transplantable tumor HeLa cells grown in Nells of plastic plates for microcultures. Single HeLa cells (target cells) are plated out on a monolayer of human embryo fibroblasts (HEF), which morphologically differ from the target cells (HeLa) and play the role of feeders.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
February 1985
The dynamics and nature of serum antibodies in experimental and natural M. pneumoniae infection have been studied. The synthesis of specific IgM, IgA and IgG has been found to occur in the course of infection.
View Article and Find Full Text PDFThe most marked production of immune interferon by human peripheral blood leukocytes and splenocytes stimulated with phytohemagglutinin (PHA) and staphylococcal enterotoxin A (SEA) was shown to be achieved when lymphoid cells are propagated under conditions of constant sparing mixing on roller apparatus at a temperature of 37 degrees +/- 0.5 degrees C. The resulting interferon was sensitive to low pH, thermolabile, inactivated by treatment with trypsin, and not neutralised by antisera to human alpha- and beta-interferons.
View Article and Find Full Text PDFPretreatment of the cultures of human peripheral blood leukocytes and splenocytes with the incubation medium of the mitogen-stimulated human lymphoid cells containing various concentrations of lymphokines (including 10 to 1280 units/ml of immune interferon) resulted in increased (by at least 4 times) production of interferon, induced by staphylococcal enterotoxin A, phytohemagglutinin and mitogen from Phytolacca americana (PWM), as well as in intensification of DNA synthesis in the producing cells. A more pronounced specific binding of the inductor labeled with radioactive iodine to the producing cells was also observed.
View Article and Find Full Text PDFThe allantoic fluid of influenza A/Hong Kong/1/68 virus-infected chick embryos was found to contain a factor enhancing guinea pig leukocyte migration. Intraperitoneal inoculation of guinea pigs with the allantoic fluid containing this factor inhibited synthesis of specific antibodies to influenza virus and sheep erythrocytes.
View Article and Find Full Text PDFInfluenza A/PR/8/34 virus was shown to suppress markedly the primary immune response of mice to sheep erythrocytes (SE). The suppressive effect of the virus was manifested by a decrease in the number of antibody-forming cells (AFC) in the spleens of the infected animals and was most marked when the mice had been infected two days before the application of the test antigen (SE). When SE and the virus were inoculated simultaneously the decrease in the AFC number was insignificant.
View Article and Find Full Text PDFIt was found that in gel filtration of a mixture of the human nasal cavity and salivary gland secretions on Sephadex G-200, three protein peaks were regularly releaved. According to the results of disk electrophoresis, the original secretion mixture consists of 11--13 fractions. The material of peak No.
View Article and Find Full Text PDFThe results of studies of the quantitative values of nonspecific antihemagglutinins of influenza viruses in the sera of birds, laboratory, wild and domestic animals (altogether 27 species) are presented. The antiviral inhibitors characterized by a number of physicochemical properties (sensitivity to heating, KIO4, trypsin, rivanol, 2-mercaptoethanol) were divided into 3 groups, sera of sheep, goats and cattle making up a separate group with regard to their sensitivity to heating and treatment with KIO4. Studies using molecular screen chromatography demonstrated the nonspecific inhibitors present in bovine sera to be heterogenous both in type (thermolabile and thermostable) and in the molecular composition.
View Article and Find Full Text PDFThe molecular composition of gamma-inhibitors in human serum was found to be heterogenous. In gel filtration of serum gamma-inhibitors were detected in macroglobulin 19S and low molecular 4S fractions. Heating at 100 degrees C for 10 min resulted in a considerable (32--128-fold) increase of the activity of 19S and 4S gamma-inhibitors.
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