Evaluation of metabolite stability in dried blood spot stored at different temperatures and times.

Dried blood spot (DBS) sampling offers significant advantages over conventional blood collection methods, such as reduced sample volume, minimal invasiveness, suitability for home-based sampling, and ease of transport. However, understanding the effects of variable storage temperatures and times on metabolite stability is crucial due to varying intervals and delivery conditions between sample collection and metabolomics analysis. To minimize biological variances, all samples were collected from the same individual simultaneously and stored at three different temperatures (4 °C, 25 °C, and 40 °C) for diverse time points (3, 7, 14, and 21 days).

Modeling the effects of plant uptake dynamics on nitrogen removal of a bioretention system.

Bioretention systems are widely used to remove nutrients from urban runoff. Plants play a key role in the nitrogen removal performance of bioretention systems; however, few model studies have focused on the effects of plant uptake dynamics on the performance. In this study, we propose an eco-hydrological model of bioretention systems by coupling plant, hydrological, and nitrogen modules.

Modeling the influences of rainfall conditions on nitrogen removal effects of a permeable pavement system.

Permeable pavement systems are important facilities for alleviating runoff pollution. However, few studies have focused on outflow nitrogen processes under varying rainfall conditions. In this study, we proposed a permeable pavement system model that can simulate the nitrogen transformation processes.

Understanding the Roles and Regulation Methods of Key Adsorption Species on Ni-Based Catalysts for Efficient Hydrogen Oxidation Reactions in Alkaline Media.

This review focuses on recent advancements in the development and understanding of nickel-based catalysts for the hydrogen oxidation reaction in alkaline media. Given the economic and environmental limitations associated with platinum group metals, nickel-based catalysts have emerged as promising alternatives due to their abundance, lower cost, and comparable catalytic properties. The review begins with an exploration of the fundamental HOR mechanisms, emphasizing the key roles of the reactive species in optimizing the catalytic activity of Ni-based catalysts.

TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer.

Recently, various cancer types have been identified to express a distinct subset of Interferon-stimulated genes (ISGs) that mediate therapy resistance. The mechanism through which cancer cells maintain prolonged Interferon stimulation effects to coordinate resistance remains unclear. Our research demonstrated that aberrant upregulation of TAGLN2 is associated with gastric cancer progression, and inhibiting its expression renders gastric cancer cells more susceptible to chemotherapy and radiation.

Prognostic relevance of ventricular arrhythmias in surgical patients with gastrointestinal tumors.

Background: Individuals diagnosed with gastrointestinal tumors are at an increased risk of developing cardiovascular diseases. Among which, ventricular arrhythmia is a prevalent clinical concern. This suggests that ventricular arrhythmias may have predictive value in the prognosis of patients with gastrointestinal tumors.

Study on Lifetime Performance Evaluation of a Precast Prestressed Concrete Frame in Chloride Environments.

This study established a comprehensive framework for evaluating the lifetime performance of precast prestressed concrete frames exposed to chloride environments. The proposed analytical framework enabled a scientifically grounded and rational assessment of both the service life and residual load-carrying capacity of precast prestressed concrete frames in chloride environments. It further served as the foundational basis for making informed decisions regarding the repair and maintenance of pertinent structures.

Exploiting Dynamic Vector-Level Operations and a 2D-Enhanced Logistic Modular Map for Efficient Chaotic Image Encryption.

Over the past few years, chaotic image encryption has gained extensive attention. Nevertheless, the current studies on chaotic image encryption still possess certain constraints. To break these constraints, we initially created a two-dimensional enhanced logistic modular map (2D-ELMM) and subsequently devised a chaotic image encryption scheme based on vector-level operations and 2D-ELMM (CIES-DVEM).

Risk factors of preoperative myocardial injury in patients with gastrointestinal tumors.

Background: Recent studies indicated that the prognosis of patients with gastrointestinal tumors is frequently influenced by its complications, notably myocardial injury. The main object is to investigate the occurrence and risk factors of myocardial injury in patients with gastrointestinal tumor.

Methods: 1126 patients who received gastrointestinal tumor related surgery from May 2018 to June 2020 in the Sixth Affiliated Hospital of Sun Yat-sen University were retrospectively collected and divided into the non-myocardial injury group and the myocardial injury group (high-sensitive cardiac troponin I (hs-cTnI) ≥ 0.

Aldosterone-stimulated endothelial epithelial sodium channel (EnNaC) plays a role in cold exposure-induced hypertension in rats.

Previous studies have demonstrated that activated endothelial epithelial sodium channel (EnNaC) impairs vasodilatation, which contributes to salt-sensitive hypertension. Here, we investigate whether mesenteric artery (MA) EnNaC is involved in cold exposure-induced hypertension (CIH) and identify the underlying mechanisms in SD rats. One group of rats was housed at room temperature and served as control.

Alcohol-dependent downregulation of apolipoprotein H exacerbates fatty liver and gut microbiota dysbiosis in mice.

Background: Alcohol-related liver disease (ALD) is a major chronic liver ailment caused by alcohol overconsumption and abuse. Apolipoprotein H (APOH) participates in lipid metabolism and might have a potential regulatory role in ALD. Therefore, this study aimed to explore the effects of ApoH on alcohol-induced liver injury and gut microbiota dysbiosis.

The therapeutic effect and mechanism of Rapamycin combined with HO-3867 on monocrotaline-induced pulmonary hypertension in rats.

This study test was designed to investigate the possible modulatory effect of rapamycin combined with HO-3867 in monocrotaline(MCT)-induced pulmonary arterial hypertension in rats. We hypothesized that combined treatment with rapamycin and HO-3867 is superior to either alone in attenuating MCT-induced rat pulmonary arterial hypertension (PAH). Pulmonary arterial hypertension was induced by a single intraperitoneal injection of monocrotaline (60 mg/kg).

The predictive value of high-sensitive troponin I for perioperative risk in patients undergoing gastrointestinal tumor surgery.

Background: The incidence of cardiovascular events in perioperative period of gastrointestinal tumor surgery cannot be ignored, and studies have shown that level of postoperative troponin is related to the postoperative risk of non-cardiac surgery. However, the relationship between pre-operative troponin levels and perioperative risk of gastrointestinal tumor surgery is unclear. Thus, we aimed to evaluate the value of high-sensitive cardiac troponin I (hs-cTnI) prior to gastrointestinal tumor surgery for perioperative risk assessment.

High-efficiency water purification for methyl orange and lead(II) by eco-friendly magnetic sulfur-doped graphene-like carbon-supported layered double oxide.

Traditional disposal techniques for the spent layered adsorbents after capturing organics suffer from intractable obstacles, such as resource waste and secondary pollution. To address this diploma, we here developed the "resource-utilization" strategy, i.e.

The Crosstalk Between Cancer Cells and Neutrophils Enhances Hepatocellular Carcinoma Metastasis via Neutrophil Extracellular Traps-Associated Cathepsin G Component: A Potential Therapeutic Target.

Background: Emerging evidences have highlighted the roles of neutrophils, as the major host microenvironment component, in the development of hepatocellular carcinoma (HCC). Neutrophils extracellular traps (NETs) produced in the infection can strengthen the behavior of cancer metastasis. Here, we investigated the roles of NETs in HCC metastasis and further explore the underlying mechanism of how NETs interact with cancer.

A novel magnesium-rich tricalcium aluminate for simultaneous removal of ammonium and phosphorus: Response surface methodology and mechanism investigation.

Coexisting ammonium (NH-N) and phosphate (PO-P) in wastewater is one of the main causes of eutrophication, which poses severe risks to aquatic ecosystem and human health worldwide. Herein, magnesium-rich tricalcium aluminate (Mg/CA), which was constructed by incorporating Mg into cement-based material CA via solid-state reaction, was employed in the simultaneous removal of NH-N and PO-P. Considering the wastewater with unbalanced N/P ratio and fluctuant pH, the effect of multiple factors (Mg/CA dosage, pH, initial contaminant concentration, and temperature) on the removal of both ions were systematically investigated by employing response surface methodology technique.

A domain-general brain network underlying emotional and cognitive interference processing: evidence from coordinate-based and functional connectivity meta-analyses.

The inability to control or inhibit emotional distractors characterizes a range of psychiatric disorders. Despite the use of a variety of task paradigms to determine the mechanisms underlying the control of emotional interference, a precise characterization of the brain regions and networks that support emotional interference processing remains elusive. Here, we performed coordinate-based and functional connectivity meta-analyses to determine the brain networks underlying emotional interference.

Activation and blockade of prelimbic 5-HT6 receptors produce different effects on depressive-like behaviors in unilateral 6-hydroxydopamine-induced Parkinson's rats.

The role of prelimbic (PrL) 5-HT6 receptors in depression is poorly understood, particularly in Parkinson's disease-related depression. Here we reported that 6-hydroxydopamine lesions in rats decreased sucrose preference and increased immobility time as measured by the sucrose preference and forced swim tests when compared to sham-operated rats, indicating the induction of depressive-like behaviors. Intra-PrL injection of 5-HT6 receptor agonist WAY208466 induced depressive-like responses in sham-operated rats, and produced antidepressant-like effects in the lesioned rats.

Identifying the Minimal Enzymes for Unusual Carbon-Sulfur Bond Formation in Thienodolin Biosynthesis.

Thienodolin (THN) features a tricyclic indole-S-hetero scaffold that encompasses two unique carbon-sulfur bonds. Although its biosynthetic gene cluster has been recently identified in Streptomyces albogriseolus, the essential enzymes for the formation of C-S bonds have been relatively unexplored. Here, we isolated and characterized a new biosynthetic gene cluster from Streptomyces sp.

Ameliorating effects of combined curcumin and desferrioxamine on 6-OHDA-induced rat mode of Parkinson's disease.

The catecholaminergic neurotoxin 6-hydroxydopamine has been widely used to mimic the lesions in dopaminergic neurons to develop Parkinson's disease. The present study was aimed to evaluate the combined treatment with Curcumin and desferrioxamine (DFO) on 6-OHDA-induced neurotoxicity in the striatum of rats. Rat models with 6-OHDA-induced Parkinson's disease were treated with curcumin, DFO, or both and the effect of different treatments on dopamine level was examined.

Enzymatic acylation of isoorientin and isovitexin from bamboo-leaf extracts with fatty acids and antiradical activity of the acylated derivatives.

This study enzymatically acrylates two flavonoids from bamboo-leaf extracts, isoorientin and isovitexin, with different fatty acids as acyl donors using Candida antarctica lipase B (CALB). The conversion yield ranged from 35 to 80% for fatty acids with different chain lengths. Higher isoorientin and isovitexin conversion yields (>75%) were obtained using lauric acid in tert-amyl-alcohol as the reaction medium.