Generation of mature kupffer cells from human induced pluripotent stem cells.

Liver macrophages, Kupffer cells (KCs), play a critical role in drug-induced liver injury (DILI) and liver diseases including cholestasis, liver fibrosis and viral hepatitis. Application of KCs in in vitro models of DILI and liver diseases is hindered due to limited source of human KCs. In vivo, KCs originate from MYB-independent macrophage progenitors, which differentiate into liver-specific macrophages in response to hepatic cues in the liver.

Deep learning enables automated scoring of liver fibrosis stages.

Current liver fibrosis scoring by computer-assisted image analytics is not fully automated as it requires manual preprocessing (segmentation and feature extraction) typically based on domain knowledge in liver pathology. Deep learning-based algorithms can potentially classify these images without the need for preprocessing through learning from a large dataset of images. We investigated the performance of classification models built using a deep learning-based algorithm pre-trained using multiple sources of images to score liver fibrosis and compared them against conventional non-deep learning-based algorithms - artificial neural networks (ANN), multinomial logistic regression (MLR), support vector machines (SVM) and random forests (RF).

Actomyosin contractility drives bile regurgitation as an early response during obstructive cholestasis.

Background & Aims: A wide range of liver diseases manifest as biliary obstruction, or cholestasis. However, the sequence of molecular events triggered as part of the early hepatocellular homeostatic response in obstructive cholestasis is poorly elucidated. Pericanalicular actin is known to accumulate during obstructive cholestasis.

Linewidth suppression mechanism of self-injection locked single-frequency fiber laser.

Linewidth suppression mechanism of the self-injection locked single-frequency fiber laser (SFFL) is investigated theoretically and experimentally. An analytical model based on the semi-phenomenological approach is built up to characterize the optical feedback in SFFL. According to the theoretical prediction, the linewidth tends to be reduced with longer external cavity photon lifetime.

600-Hz linewidth short-linear-cavity fiber laser.

We proposed a short-linear-cavity (SLC) fiber laser based on a virtual-folded-ring (VFR) resonator and a fiber Bragg grating Fabry-Perot filter. Spatial hole burning effect was reduced by retarding the polarization state of the counter-propagating light waves utilizing the VFR structure. The photon lifetime of the resonator was extended due to the multi-reflection inside the FBG FP, which increased the intra-cavity power and relatively suppressed the contribution of phase diffusion from spontaneous emission.

High OSNR watt-level single-frequency one-stage PM-MOPA fiber laser at 1083 nm.

A 1.03 W optical signal-to-noise ratio (OSNR) of > 70 dB single-frequency polarization-maintained master-oscillator power amplifier (PM-MOPA) laser at 1083 nm was demonstrated. The seed laser of this laser system was a distributed Bragg reflector short cavity Yb-doped phosphate fiber oscillator.

1.95 μm kHz-linewidth single-frequency fiber laser using self-developed heavily Tm3+-doped germanate glass fiber.

We demonstrated a kHz-linewidth single-frequency laser at 1.95 μm using the self-developed heavily Tm(3+)-doped single-mode germanate glass fiber with the net gain coefficient of 2.3 dB per centimeter.

10.9 W kHz-linewidth one-stage all-fiber linearly-polarized MOPA laser at 1560 nm.

An all-fiber 10.9 W single-frequency one-stage linearly-polarized master-oscillator power amplifier (MOPA) laser at 1560 nm has been demonstrated. The laser linewidth is less than 3.

A 1014 nm linearly polarized low noise narrow-linewidth single-frequency fiber laser.

We present the demonstration of a compact linearly polarized low noise narrow-linewidth single-frequency fiber laser at 1014 nm. The compact fiber laser is based on a 5-mm-long homemade Yb(3+)-doped phosphate fiber. Over 164 mW stable continuous-wave single transverse and longitudinal mode lasing at 1014 nm has been achieved.

Low noise single-frequency single-polarization ytterbium-doped phosphate fiber laser at 1083 nm.

We present a low noise single-frequency and single-polarization distributed Bragg reflector fiber laser at 1083 nm by using a 1.8 cm long newly developed ytterbium-doped phosphate single mode glass fiber. The maximum output power is more than 100 mW with a slope efficiency of >29.