Multifunctional electrospinning periosteum: Development status and prospect.

In the repair of large bone defects, loss of the periosteum can result in diminished osteoinductive activity, nonunion, and incomplete regeneration of the bone structure, ultimately compromising the efficiency of bone regeneration. Therefore, the research and development of tissue-engineered periosteum which can replace the periosteum function has become the focus of current research. The functionalized electrospinning periosteum is expected to mimic the natural periosteum and enhance bone repair processes more effectively.

The Preparation and Effects of Organic-Inorganic Antioxidative Biomaterials for Bone Repair.

Reactive oxygen species (ROS) has great influence in many physiological or pathological processes in organisms. In the site of bone defects, the overproduced ROS significantly affects the dynamic balance process of bone regeneration. Many antioxidative organic and inorganic antioxidants showed good osteogenic ability, which has been widely used for bone repair.

Mussel inspired multifunctional bovine serum albumin (BSA) coatings loaded with Baicalein (BAI) to enhance osteogenesis and resist oxidative stress for potential application on implant.

Oxidative stress is associated with most traumatic or pathological bone defects, and seriously affects the effect of implantation. The construction of antioxidative and osteogenic coatings is of great significance to accelerate the bone regeneration of implants. In this study, baicalein (BAI), a nature flavonoid drug, was loaded in bovine serum albumin (BSA) by desolvent method to prepare BAI-BSA composite protein, and tannic acid (TA)/BAI-BSA coatings were further built via layer by layer self-assembly technology.

Universal Strategy to Efficiently Coat Zeolitic Imidazolate Frameworks onto Diverse Substrates.

Anchoring metal-organic framework (MOF) coating has attracted extensive interest due to its wide applications in drug delivery, gas storage and separation, catalysis, and so forth. Here, we reported a flexible strategy on generating ZIF-8 coatings onto diverse substrates in the scale up to hundreds cm, independent of the geometry of the substrate, with controllable thickness, texture structure, and crystal size of coating. By understanding the mechanism and factors on the formation of ZIF-8 coatings, various zeolitic imidazolate framework coatings were successfully produced.

Poly(tannic acid) nanocoating based surface modification for construction of multifunctional composite CeONZs to enhance cell proliferation and antioxidative viability of preosteoblasts.

Ceria (CeO) based materials possess many antioxidant enzyme-like activities and unique properties for bone repair, but their free radical scavenging function is still insufficient. In order to deal with the complex oxidative stress environment in bone repair, multifunctional composite CeO nanozymes (CeONZs), featuring multiple antioxidative properties, were constructed surface modification on CeONZs with nanoscale poly(tannic acid) (PTA) coatings. Moreover, we adjusted pH conditions (ranging from 4 to 9) to effectively control the formation and antioxidative properties of PTA coatings on CeONZ surfaces.

Multifunctional Tannic Acid (TA) and Lysozyme (Lys) Films Built Layer by Layer for Potential Application on Implant Coating.

A multifunctional (TA/Lys) film, featuring good antioxidant property, fast cell attachment at the initial stage, enhanced osteogenesis, and broad-spectrum antibacterial property, was constructed by the layer-by-layer (LBL) method. The building process was monitored by quartz crystal microbalance with dissipation (QCM-D); the physical properties, such as topography, stiffness in dry and liquid state, and conformation of Lys in the film, were thoroughly characterized. These physical properties were modulated by varying the salt concentration at which the film was constructed.

Building polyphenol and gelatin films as implant coating, evaluating from in vitro and in vivo performances.

Bone related implants have huge potential market in global. Improving the implant outcomes and probability of implant success are highly pursued to relieve the pain of patients and burden on native healthy system. There are growing evidence to support reactive oxygen species (ROS) directly involved in bone diseases and failure of implants.

Graphene Oxide and Lysozyme Ultrathin Films with Strong Antibacterial and Enhanced Osteogenesis.

There is a great demand worldwide for bone-related implant materials. The drawbacks of chronic infections and poor bone healing of current implant materials have limited their clinical applications. Functionalizing the implant surfaces with antibacterial and osteogenic films on implant materials provides new opportunities for fabricating novel implant materials.

Berberine attenuates sodium palmitate-induced lipid accumulation, oxidative stress and apoptosis in grass carp(Ctenopharyngodon idella)hepatocyte in vitro.

The objective of this work was to investigate the effect of berberine (BBR) on the Cell viability, lipid accumulation, apoptosis, cytochrome c, caspase-9 and caspase-3 in lipid accumulation-hepatocytes induced by sodium palmitate in vitro. The lipid accumulation-hepatocytes (induced by 0.5 mM sodium palmitate for 24 h) were treated with 5 μM berberine for 12 h.

A bio-inspired, one-step but versatile coating onto various substrates with strong antibacterial and enhanced osteogenesis.

It is of great interest to prepare osteogenic and antibacterial coatings for successful implants. Current coating techniques suffer from being time-consuming, substrate material or shape dependence, expensive equipment, environmental pollution, low stability, processes that are difficult to control, etc. Herein, inspired by mussels, we report a one-step and versatile method to fabricate a dual functional coating.

Molecular characterization of microtubule-associated protein 1-light chain 3B in Megalobrama amblycephala fed with high fat/berberine diets.

This study tested the effect of berberine on autophagy-related protein of Megalobrama amblycephala fed with high fat diet under different feeding modes. The full-length complementary DNA (cDNA) of microtubule-associated protein 1-light chain 3B (LC3B) was 1871 bp with an open reading frame of 378 bp encoding 125 amino acids. High homology at nucleotide and amino acid sequences to carp LC3B was revealed though sequence analysis.

N-carboxyethyl chitosan fibers prepared as potential use in tissue engineering.

To improve the hydrophilicity of chitosan fiber, N-carboxyethyl chitosan fiber was prepared through Michael addition between chitosan fiber with acrylic acid. The structure was studied by (1)H NMR. The degree of N-substitution, measured via (1)H NMR, was easily varied from 0.