Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors.

Background: The interface zone, area around invasive carcinoma, can be thought of as the actual tissue of the tumor microenvironment with precedent alterations for tumor invasion. However, the heterogeneity and characteristics of the microenvironment in the interface area have not yet been thoroughly explored.

Methods: For in vitro studies, single-cell RNA sequencing (scRNA-seq) was used to characterize the cells from the tumor zone, the normal zone and the interface zone with 5-mm-wide belts between the tumor invasion front and the normal zone.

Green synthesis of silver nanoparticles from extracts and characterization of its inhibitory effect on Chinese cabbage soft rot pathogen.

The pathogenic bacterium causes soft rot in cabbage and significantly reduces plant yield. In this study, we employed extracts to synthesis silver nanoparticles (Mf-AgNPs) and investigated their functions against . The results showed that the surface plasmon resonance (SPR) peak of AgNP was 412 nm under optimal synthesis conditions.

Preferential expression of cytochrome CYP CYP2R1 but not CYP1B1 in human cord blood hematopoietic stem and progenitor cells.

Cytochrome P450 (CYP) enzymes metabolize numerous endogenous substrates, such as retinoids, androgens, estrogens and vitamin D, that can modulate important cellular processes, including proliferation, differentiation and apoptosis. The aim of this study is to characterize the expression of CYP genes in CD34+ human cord blood hematopoietic stem and early progenitor cells (CBHSPCs) as a first step toward assessment of the potential biological functions of CYP enzymes in regulating the expansion or differentiation of these cells. CD34+ CBHSPCs were purified from umbilical cord blood via antibody affinity chromatography.