DNA-Assisted CRISPR-Cas12a Enhanced Fluorescent Assay for Protein Detection in Complicated Matrices.

Proteins are important biological macromolecules that perform a wide variety of functions in the cell and human body, and can serve as important biomarkers for early diagnosis and prognosis of human diseases as well as monitoring the effectiveness of disease treatment. Hence, sensitive and accurate detection of proteins in human biospecimens is imperative. However, at present, there is no ideal method available for the detection of proteins in clinical samples, many of which are present at ultralow (less than 1 pM) concentrations and in complicated matrices.

A STT3A-dependent PD-L1 glycosylation modification mediated by GMPS drives tumor immune evasion in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is a malignant tumor characterized by rapid progression. To explore the regulatory mechanism of rapid tumor growth and metastasis, we conducted proteomic and scRNA-Seq analyses on advanced HCC tissues and identified a significant molecule, guanine monophosphate synthase (GMPS), closely associated with the immune evasion in HCC. We analyzed the immune microenvironment characteristics remodeled by GMPS using scRNA-Seq and found GMPS induced tumor immune evasion in HCC by impairing the tumor-killing function of CD8  T cells.

Identification of Candidate Hub Genes and Drug Targets for Cholangiocarcinoma via Juhua (Chrysanthemum Morifolium) Bioactivity and Molecular Docking: A Bioinformatics Approach.

Background: Cholangiocarcinoma (CHOL) is a malignancy with poor clinical outcomes and limited treatment options. While extensive research has investigated genetic and signaling pathways in CHOL, the molecular mechanisms underlying disease pathogenesis remain incompletely understood. A key hurdle has been the lack of a systematic, multi-omic approach to illuminate causal relationships between genetic variants and CHOL risk.

Bioavailability and Bioaccessibility of Iodine in Edible Seaweeds: Method Development and Health Implications.

Both iodine (I) deficiency and I excess can adversely affect human health. Seaweed consumption is one of the most important natural sources for I. This study assessed I relative bioavailability (RBA) in seaweed using an mouse model and compared it with I bioaccessibility measured by three methods (Physiologically Based Extraction Test (PBET), the Solubility Bioaccessibility Research Consortium (SBRC), and the Unified Bioaccessibility Research Group of Europe Method (UBM)).

Navigating (Dis)empowerment: Dialectical Tensions in the Enactment of Resilience of College Students with Eating Disorders.

Research has shown a dramatic increase of eating disorders (EDs) among young people during disruptive times. Understanding the role of communication in impeding or enacting resilience not only helps those with EDs develop better strategies for coping and changing their lives but can also inform effective interventions at familial, community, and system levels. Guided by the communication theory of resilience (CTR), our study explores how college students with EDs enacted resilience through recalled interactions with parents, friends, community members, and health professionals during the COVID-19 pandemic.

Lenalidomide-induced pure red cell aplasia is associated with elevated expression of MHC-I molecules on erythrocytes.

The RVd therapy, combining lenalidomide, bortezomib, and dexamethasone, is a mainstay treatment for multiple myeloma. A multiple myeloma patient developed pure red cell aplasia (PRCA) following RVd treatment, despite the absence of common PRCA triggers. In vitro analyses reveal lenalidomide as a pivotal disruptor of erythropoiesis.

Sensitive and specific detection of trace Al ions using an upconversion nanoparticle-xylenol orange complex the inner filter effect.

We have developed a novel fluorescence sensor based on upconversion nanoparticles (UCNPs) for the rapid and sensitive detection of trace aluminum ions (Al). The sensor utilizes the inner filter effect (IFE) between the UCNPs and the xylenol orange-aluminum complex (XO-Al), resulting in significant fluorescence quenching at 543 nm upon Al binding. This quenching correlates directly with the Al concentration, allowing for quantitative detection within a range of 0-30 μM and achieving an ultra-low detection limit of 0.

Initial Evaluation of Acceptability, Engagement, and Effectiveness of the MO App to Provide Tailored and Comprehensive Support for Smoking Cessation: Development and Usability Study.

Background: Despite the growing availability of smoking cessation apps, low engagement and cessation rates have remained a significant challenge. To address this issue, we used a user-centered design to iteratively develop a mobile app (MO) to provide comprehensive, tailored, and evidence-based content to support smokers in their quitting journey.

Objective: This study examined the acceptability, use, and preliminary efficacy of the MO app for smoking cessation.

Molecular sandwich-based DNAzyme catalytic reaction towards transducing efficient nanopore electrical detection of antigen proteins.

Despite significant advances in nanopore nucleic acid sequencing and sensing, protein detection remains challenging due to the inherent complexity of protein molecular properties (, net charges, polarity, molecular conformation & dimension) and sophisticated environmental parameters (, biofluids), resulting in unsatisfactory electrical signal resolution for protein detection such as poor accessibility, selectivity and sensitivity. The selection of an appropriate electroanalytical approach is strongly desired which should be capable of offering easily detectable and readable signals regarding proteins particularly depending on the practical application. Herein, a molecular sandwich-based cooperative DNAzyme catalytic reaction nanopore detecting approach was designed.

Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein.

C-reactive protein (CRP) is a protein made by the liver, which is released into the bloodstream in response to inflammation. Furthermore, CRP is a potential risk factor for heart disease. Hence, it is of great importance to develop a rapid, sensitive, accurate, and cost-effective method for CRP detection.

Identification of Wheat Glutamate Synthetase Gene Family and Expression Analysis under Nitrogen Stress.

Nitrogen (N), as the main component of biological macromolecules, maintains the basic process of plant growth and development. GOGAT, as a key enzyme in the N assimilation process, catalyzes α-ketoglutaric acid and glutamine to form glutamate. In this study, six genes in wheat ( L.

Can co-ordination advancement of two-way FDI improve resource misallocation? Evidence from 285 cities in China.

During the past 40 years of reform and opening-up, the implementation of an unbalanced enhancement strategy has led to severe resource misallocation, making the mitigation and rectification of this misallocation an urgent issue. This paper utilizes urban data from China between 2006 and 2020 to examine the impact of two-way foreign direct investment (FDI) on resource misallocation, as well as the pathways through which coordinated advancement of two-way FDI affects resource misallocation. After undergoing a series of robustness and endogeneity tests, the conclusion remains stable.

Acetylation-dependent regulation of core spliceosome modulates hepatocellular carcinoma cassette exons and sensitivity to PARP inhibitors.

Despite the importance of spliceosome core components in cellular processes, their roles in cancer development, including hepatocellular carcinoma (HCC), remain poorly understood. In this study, we uncover a critical role for SmD2, a core component of the spliceosome machinery, in modulating DNA damage in HCC through its impact on BRCA1/FANC cassette exons and expression. Our findings reveal that SmD2 depletion sensitizes HCC cells to PARP inhibitors, expanding the potential therapeutic targets.

Single-Cell Sensitive Colorimetric pH Detection Based on Microscope Ratiometric Grayscale.

Rapid and accurate identification of the intracellular pH is critical in the field of biomedicine. In this work, we effectively identified and quantified the intracellular pH and its distribution at the single-cell level using an image sensor based on an ordinary bright-field optical microscope that divided the cell staining images into their red (R) and blue (B) channels. The grayscale of the R and B channels was subjected to a ratiometric operation to generate ratiometric grayscale cell images of the microscope.

Maternal exposure to DON during lactation induces testicular toxicity in pubertal and adult offspring mice.

Deoxynivalenol (DON), a type B trichothecene mycotoxin, commonly occurs in cereal grains, and poses significant health risks to humans and animals. Numerous studies reveal its obvious toxic effects on male reproductive performance as well as its ability to transfer from the lactating mother to the suckling offspring through colostrum and milk. The objective of this study was to evaluate the toxic effect of lactational DON exposure on testicular morphology, hormonal levels, inflammation, apoptosis and proliferation of germ cells, tight junction, and sperm quality in male offspring.

Structural characterization and immunostimulant activities of polysaccharides fractionated by gradient ethanol precipitation method from C. A. Meyer.

C. A. Meyer is a dual-purpose plant for medicine and food, its polysaccharide is considered as an immune enhancer.

MiR-186-5p prevents hepatocellular carcinoma progression by targeting methyltransferase-like 3 that regulates m6A-mediated stabilization of follistatin-like 5.

Background: Hepatocellular carcinoma (HCC) is a multistep process involving sophisticated genetic, epigenetic, and transcriptional changes. However, studies on microRNA (miRNA)'s regulatory effects of N6-methyladenosine (m6A) modifications on HCC progression are limited.

Methods: Cell Counting Kit-8 (CCK-8), clone formation, and Transwell assays were used to investigate changes in cancer cell proliferation, invasion, and migration.

Hepatocellular Carcinoma LINC01116 Outcompetes T Cells for Linoleic Acid and Accelerates Tumor Progression.

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer with a highly immunosuppressive tumor microenvironment and a typical pattern of disturbances in hepatic lipid metabolism. Long non-coding RNAs are shown to play an important role in the regulation of gene expression, but much remains unknown between tumor microenvironment and lipid metabolism as a bridging molecule. Here, long intergenic nonprotein coding RNA 01116 (LINC01116) acts as this molecular which is frequently upregulated in HCC patients and associated with HCC progression in vitro and in vivo is identified.

Neddylation inhibition affects early embryonic development by disrupting maternal-to-zygotic transition and mitochondrial function in mice.

Post-translational modifications (PTMs) are critical for early development in mice because early cleavage-stage embryos are characterized by transcriptional inactivity. Neddylation is an important ubiquitin-like PTM that regulates multiple biophysical processes. However, the exact roles of neddylation in regulating early embryonic development remain largely unknown.