Publications by authors named "Shuo Zheng"

Objective: This study aimed to investigate the function of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplet accumulation in myotubes.

Methods: The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, RT-qPCR, immunoblotting, immunofluorescence staining, and Oil Red O staining.

Results: Analysis of porcine BodyMap transcriptomic data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue.

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Pre-pregnancy obesity (PPO) seriously threatens the health of both mother and offspring. Pre-pregnancy weight management is particularly important for the prevention of metabolic diseases in offspring. Semaglutide is one of the most effective glucagon-like peptide-1 agonizts for the management of obesity and metabolic diseases, but little is known about its effect on the long-term health of offspring.

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Purpose: Limb ischemia is a refractory disease characterized by insufficient angiogenesis and tissue necrosis. Currently, the primary clinical treatment method is surgical intervention; however, the prognosis for patients with severe limb ischemia remains unsatisfactory. Although some studies have evaluated the effects of using bioactive factors to promote neovascularization and tissue repair, the clinical outcomes have not met expectations, possibly due to the difficulties in maintaining biological activity and avoiding potential side effects.

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Nutritional intake is closely linked to gonadal development, although the mechanisms by which food intake affects gonadal development are not fully understood. Cholecystokinin (CCK) is a satiety neuropeptide derived from the hypothalamus, and the present study observed that hypothalamic CCK expression is significantly influenced by food intake, which is mediated through blood glucose levels. Interestingly, CCK and its receptors were observed to exhibit a high expression in the hypothalamus-pituitary-gonad (HPG) axis of grass carp (), suggesting that CCK is potentially involved in regulating fish reproduction through the HPG axis.

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To investigate the clinical characteristics of patients with hydrocephalus after hypertensive cerebral haemorrhage (HICH) and to analyse the relevant factors affecting the prognosis. A total of 500 patients with HICH admitted to the neurosurgery department of The First Hospital of Hebei Medical University between January 2020 and July 2024 were retrospectively analysed. The clinical data of the patients were collected, the occurrence of hydrocephalus within 3 months after discharge was followed up, and the patients were divided into the occurrence group and the non-occurrence group.

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Neutrophil elastase (NE) has been reported to be a pro-inflammatory stimulus for macrophages. The aim of the present study was to determine the impact of NE exposure on the human macrophage proteome and evaluate its impact on pro-inflammatory signals. Human blood monocytes from healthy volunteers were differentiated to macrophages and then exposed to either 500 nM of NE or control vehicle for 2 h in triplicate.

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Cellulose, the most abundant biomass, is highly appreciated for its robustness, biodegradability, and renewability, garnering significant interest for innovative applications in sustainable functional materials. Composites of cellulose and polyaniline (PANI) are particularly promising for flexible supercapacitors because of their ease of processing, excellent electrical conductivity, and high theoretical specific capacitance. However, challenges persist due to the tendency of PANI to agglomerate and the weak interfacial interactions between PANI and cellulose fibers (CFs).

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Neutrophil elastase (NE), elevated in the cystic fibrosis (CF) airway, causes macrophage phagocytic failure. We previously reported that NE increases the release of protease calcium ion-dependent papain-like cysteine protease-2 (Calpain-2) in macrophages. We hypothesized that NE mediates macrophage failure through activation of Calpains.

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Background And Aims: Vascular smooth muscle cell (VSMC) phenotype switching is a pathological hallmark in various cardiovascular diseases. N4-acetylcytidine (ac4C) catalyzed by N-acetyltransferase 10 (NAT10) is well conserved in the enzymatic modification of ribonucleic acid (RNA). NAT10-mediated ac4C acetylation is involved in various physiological and pathological processes, including cardiac remodelling.

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The aetiological agent of porcine reproductive and respiratory syndrome, a deadly disease that affects pigs and seriously jeopardises the global swine industry, is a porcine reproductive and respiratory syndrome virus (PRRSV). Tylvalosin tartrate, which is a macrolide antibiotic, is the active ingredient in Aivlosin. In recent years, tylvalosin tartrate has widely been used to control porcine reproductive and respiratory syndrome in swine herds in China.

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Article Synopsis
  • * Low temperatures increased the expression of certain stress-related genes while inhibiting reproductive hormone genes, leading to reduced sperm count and motility, indicating a disruption in testicular function and spermatogenesis.
  • * Metabolomics analysis revealed lower levels of key metabolites related to energy production in the testes after exposure to low temperatures, suggesting that cold stress affects sperm vitality by impairing energy metabolism and testicular development.
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The aim of this study was to investigate the mechanism of itaconate's potential effect in diabetic kidney disease. Renal immune responsive gene 1 (IRG1) levels were measured in db/db mice and streptozotocin (STZ) + high-fat diet (HFD)-induced diabetic mice. knockout mice were generated.

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  • Emerging research suggests that human umbilical cord mesenchymal stem cells (UC-MSCs) may help treat chemotherapy-induced premature ovarian failure (POF) by improving ovarian function and reducing cell death.
  • The study evaluated the timing of UC-MSC treatment and its effects on ovarian health in a mouse model, finding that administering UC-MSCs on day 6 post-chemotherapy significantly enhanced ovarian function compared to later timing.
  • UC-MSC-derived extracellular vesicles (EVs) demonstrated similar therapeutic effects, activating the PI3K/AKT signaling pathway to prevent apoptosis of ovarian cells, with clusterin (CLU) identified as a key protein in this protective mechanism.
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  • The study explores the role of Zinc-alpha2-glycoprotein (ZAG) in hypertension, focusing on its relationship with blood pressure regulation and metabolic disorders.
  • Researchers found that hypertensive participants had lower serum ZAG levels, and experiments with Azgp1-/- mice showed increased blood pressure linked to impaired sodium excretion and altered renal lipid metabolism.
  • Interventions, including ZAG protein administration and NHE inhibitors, demonstrated potential therapeutic avenues for improving blood pressure and sodium handling in hypertension.
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The neonatal mammalian heart can regenerate following injury through cardiomyocyte proliferation but loses this potential by postnatal day 7. Stimulating adult cardiomyocytes to reenter the cell cycle remains unclear. Here we show that cardiomyocyte proliferation depends on its metabolic state.

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Programmed death-ligand 1 (PD-L1) on tumor-derived small extracellular vesicles (sEVs) limits therapeutic effectiveness by interacting with the PD-1 receptor on host immune cells. Targeting the secretion of sEV PD-L1 has emerged as a promising strategy to enhance immunotherapy. However, the lack of small-molecule inhibitors poses a challenge for clinical translation.

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DNA polymerase ζ (Pol ζ) plays an essential role in replicating damaged DNA templates but contributes to mutagenesis due to its low fidelity. Therefore, ensuring tight control of Pol ζ's activity is critical for continuous and accurate DNA replication, yet the specific mechanisms remain unclear. This study reveals a regulation mechanism of Pol ζ activity in human cells.

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Aim: To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts (HPFs) and its correlation with the severity grades of pterygium.

Methods: Pterygium and normal conjunctival tissues were collected from the superior area of the same patient's eye (=33). The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reverse-transcription polymerase chain reaction (RT-qPCR) were analyzed.

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  • A study was conducted to compare the effectiveness and safety of two holmium laser techniques—conventional (basketing + dusting) versus Moses (pop-dusting)—in treating 2-3 cm kidney stones in 230 patients who underwent flexible ureteroscopy.
  • Both methods resulted in similar stone-free rates and complications, but the Moses technique used significantly higher laser energy and reduced operation time compared to the conventional method.
  • The study concluded that while both laser modes are effective for treating renal calculi, the Moses mode is preferred for isolated stones, whereas both methods have similar efficiency when treating multiple stones.
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Background: Interstitial cystitis/bladder pain syndrome (IC/BPS) is a common chronic disease, and its aetiology and pathogenesis remain unclear. This study aimed to identify potential urine and serum biomarkers in patients with IC/BPS to further understand the pathogenesis and diagnosis of the disease.

Methods: Patients with IC/BPS diagnosed and treated in the First Hospital of Hebei Medical University from 1 July 2021 to 30 July 2023 were selected.

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Background: Renal cell carcinoma (RCC), a common and highly invasive malignant tumour, presents clinical challenges due to its propensity for easy metastasis. Inferior vena cava tumour thrombus is a common RCC complication significantly impacting patient prognosis. This study investigates C-X-C chemokine receptor type 2 ()/-induced epithelial-mesenchymal transition (EMT) in RCC with inferior vena cava tumour thrombus.

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Neutrophil elastase (NE) is taken up by macrophages, retains intracellular protease activity, and induces a pro-inflammatory phenotype. However, the mechanism of NE-induced pro-inflammatory polarization of macrophages is not well understood. We hypothesized that intracellular NE degrades histone deacetylases (HDAC) and Sirtuins, disrupting the balance of lysine acetylation and deacetylation and resulting in nuclear to cytoplasmic translocation of a major alarmin, High Mobility Group Box 1 (HMGB1), a pro-inflammatory response in macrophages.

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Article Synopsis
  • NUCB2 has oncogenic properties and its role in osteosarcoma is not fully understood, but this study reveals that its levels rise under glucose deprivation stress.
  • Increased NUCB2 protects osteosarcoma cells from death caused by glucose deprivation, while its depletion decreases cell growth and spread, also affecting immune responses.
  • The research highlights that NUCB2 promotes osteosarcoma progression through a pathway involving NUCKS1 and CXCL8, suggesting that targeting NUCB2 and combining it with anti-PD-L1 treatment could enhance immunotherapy effectiveness.
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Nano zero-valent iron (nZVI) is an advanced environmental functional material for the degradation of tetrabromobisphenol A (TBBPA). However, high surface energy, self-agglomeration and low electron selectivity limit degradation rate and complete debromination of bare nZVI. Herein, we presented biomass-derived cellulose nanocrystals (CNC) modified nZVI (CNC/nZVI) for enhanced TBBPA removal.

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Homeobox B9 (HOXB9) has been shown to play a critical role in several tumors. However, the precise biological mechanisms and functions of HOXB9 in osteosarcoma remain largely unknown. In this study, we found that HOXB9 was increased upon glucose starvation.

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