Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation.

Vascularization as a spatiotemporally interlaced process involving angiogenesis and vascular remodeling, has seldom been investigated comprehensively regarding the interrelationship of the two intertwining but sequential processes. Here, a shortwave infrared (SWIR) fluorescence imaging strategy based on quantum dots (QDs) was designed to dynamically visualize vascularization in vivo and in situ in a perforator transplantation mouse model. The vascularization process could be directly perceived from the established flap model with an optimal observation window at 10 min post-injection.

Unsupervised translation of vascular masks to NIR-II fluorescence images using Attention-Guided generative adversarial networks.

The second near-infrared window (NIR-II) fluorescence imaging is a crucial technology for investigating the structure and functionality of blood vessels. However, challenges arise from privacy concerns and the significant effort needed for data annotation, complicating the acquisition of near-infrared vascular imaging datasets. To tackle these issues, methods based on deep learning for data synthesis have demonstrated promise in generating high-quality synthetic images.

A systematic study on composition and antioxidant of 6 varieties of highbush blueberries by 3 soil matrixes in China.

Blueberries are the most popular small berries, in order to solve the problem of unbalanced blueberry resources in different regions of China. In this study, 18 blueberries were analyzed by chromatography and mass spectrometry for 9 soil elements, 6 anthocyanins, 7 phenolic acids, 9 organic acids, and 12 flavonoids. The result showed that blueberry physico-chemical indicators were significantly variable across production regions by Wenn and volcano maps, chlorogenic acid, ascorbic acid, citric acid, catechin were the main antioxidant active components, soil pH was significantly correlated with low content of anthocyanins and organic acids, soil elements were not significantly correlated with fruits antioxidant activity by the network correlation analysis.

A phosphoglycerate mutase 1 allosteric inhibitor restrains TAM-mediated colon cancer progression.

Colorectal cancer (CRC) is a prevalent malignant tumor often leading to liver metastasis and mortality. Despite some success with PD-1/PD-L1 immunotherapy, the response rate for colon cancer patients remains relatively low. This is closely related to the immunosuppressive tumor microenvironment mediated by tumor-associated macrophages (TAMs).

Fe(III)-Aided sp. ES2-1 Regulates Molecular Mechanisms of 17β-Estradiol Biodegradation.

17β-estradiol (E2) is one of the strongest environmental estrogens threatening wildlife and human health globally. Microbial degradation is an alternative strategy to remediate E2-contaminated sites and may be regulated by ubiquitous Fe(III) in eco-environments. We have previously obtained a high-efficiency E2 degrader, sp.

Synchronously in vivo real-time monitoring bacterial load and temperature with evaluating immune response to decipher bacterial infection.

Determining the precise course of bacterial infection requires abundant in vivo real-time data. Synchronous monitoring of the bacterial load, temperature, and immune response can satisfy the shortage of real-time in vivo data. Here, we conducted a study in the joint-infected mouse model to synchronously monitor the bacterial load, temperature, and immune response using the second near-infrared (NIR-II) fluorescence imaging, infrared thermography, and immune response analysis for 2 weeks.

Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in .

, a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China, possesses considerable aquacultural and economic potential. However, the species faces challenges due to significant variation in the gonadal development rate among females, resulting in inconsistent ovarian maturation times at the population level, an extended reproductive period, and limitations on fish growth rate due to ovarian prematurity. In the present study, we combined genome-wide association study (GWAS) and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms (SNPs) and candidate genes associated with population-asynchronous ovarian development in .

Chromosome-level genome assembly of the yellow-cheek carp Elopichthys bambusa.

Yellow-cheek carp (Elopichthys bambusa) is a typical large and ferocious carnivorous fish endemic to East Asia, with high growth rate, nutritional value and economic value. In this study, a chromosome-level genome of yellow-cheek carp was generated by combining PacBio reads, Illumina reads and Hi-C data. The genome size is 827.

Laccase-induced decontamination and humification mechanisms of estrogen in water-crop matrices.

Enzymatic humification plays a crucial biogeochemical role in eliminating steroidal estrogens and expanding organic carbon stocks. Estrogenic contaminants in agroecosystems can be taken up and acropetally translocated by crops, but the roles of laccase-triggered rhizospheric humification (L-TRH) in pollutant dissipation and plant uptake remain poorly understood. In this study, the laccase-induced decontamination and humification mechanisms of 17β-estradiol (E2) in water-crop media were investigated by performing greenhouse pot experiments with maize seedlings ( L.

Lignin precursors enhance exolaccase-started humification of bisphenol A to form functional polymers.

Humification plays a significant role in converting phenolic pollutants and forming heterogeneous polymers, but few studies have been performed to investigate exolaccase-started humification (ESH). Herein, the influences of lignin precursors (LPs) on exolaccase-induced bisphenol A (BPA) removal and humification were explored. In particular, the architectural features and botanical effects of the formed humification products were also tested.

Advances in Biosynthesis of Non-Canonical Amino Acids (ncAAs) and the Methods of ncAAs Incorporation into Proteins.

The functional pool of canonical amino acids (cAAs) has been enriched through the emergence of non-canonical amino acids (ncAAs). NcAAs play a crucial role in the production of various pharmaceuticals. The biosynthesis of ncAAs has emerged as an alternative to traditional chemical synthesis due to its environmental friendliness and high efficiency.

Adaptive responses and metabolic strategies of Novosphingobium sp. ES2-1-17β-estradiol analyzed through integration of genomic and proteomic approaches.

Environmental 17β-estradiol (E2) can cause potential harm to ecological balance and human health. Novosphingobium sp. ES2-1 is an E2-degrading bacterium previously obtained, which converts E2 to estrone (E1) and then to 4-hydroxyestrone (4-OH-E1) followed by oxidation to form metabolites with long-chain structure during upstream degradation.

Exolaccase Propels Humification to Decontaminate Bisphenol A and Create Humic-like Biostimulants.

Exolaccase-propelled humification (E-PH) helps eliminate phenolic pollutants and produce macromolecular precipitates. Herein, we investigated the influencing mechanism of 12 humic precursors (HPs) on exolaccase-enabled bisphenol A (BPA) decontamination and humification. Catechol, vanillic acid, caffeic acid, and gentian acid not only expedited BPA removal but also created large amounts of copolymeric precipitates.

AHR, a novel inhibitory immune checkpoint receptor, is a potential therapeutic target for chemoresistant glioblastoma.

Purpose: This study aims to elucidate the mechanism underlying temozolomide resistance in patients with MGMT promoter hypomethylated glioblastoma, which is correlated with poor prognosis. The objective is to identify therapeutic targets and drugs suitable for temozolomide-resistant glioblastoma patients using big data analysis.

Methods: In this retrospective study, transcriptome sequencing data from 457 glioblastoma patients, multi-omics data, and single-cell sequencing data were employed to assess the expression pattern, prognostic value, and biological functions of AHR in glioblastoma.

Spatio-temporally deciphering peripheral nerve regeneration after extracellular vesicle therapy under NIR-II fluorescence imaging.

Extracellular vesicles (EVs) show potential as a therapeutic tool for peripheral nerve injury (PNI), promoting neurological regeneration. However, there are limited data on the spatio-temporal trafficking and biodistribution of EVs. In this study, we introduce a new non-invasive near-infrared fluorescence imaging strategy based on glucose-conjugated quantum dot (QDs-Glu) labeling to target and track EVs in a sciatic nerve injury rat model in real-time.

Initial Studies on the Effect of the Rice-Duck-Crayfish Ecological Co-Culture System on Physical, Chemical, and Microbiological Properties of Soils: A Field Case Study in Chaohu Lake Basin, Southeast China.

Rice-duck and rice-crayfish co-culture patterns can increase soil productivity and sustainability and reduce the use of chemical pesticides and fertilizers, thereby reducing the resulting negative environmental impacts. However, most studies have focused on the rice-duck and rice-crayfish binary patterns and have ignored integrated systems (three or more), which may have unexpected synergistic effects. To test these effects, a paddy field experiment was carried out in the Chaohu Lake Basin, Hefei city, Southeast China.

Biotransformation of bisphenol A in vivo and in vitro by laccase-producing Trametes hirsuta La-7: Kinetics, products, and mechanisms.

Bisphenol A (BPA) is a ubiquitous endocrine disruptor that poses adverse human health risks. Herein, biotransformation kinetics, products, and mechanisms of BPA undergoing a laccase-producing Trametes hirsuta La-7 metabolism were for the first time reported. Strain La-7 could completely biotransform ≤0.

Aerobic Degradation Characteristics and Mechanism of Decabromodiphenyl Ether (BDE-209) Using Complex Bacteria Communities.

Complex bacteria communities that comprised sp. (M1) and sp. (M2) with effective abilities of degrading decabromodiphenyl ether (BDE-209) were investigated for their degradation characteristics and mechanisms under aerobic conditions.

Exolaccase-boosted humification for agricultural applications.

Globally, phenolic contaminants have posed a considerable threat to agro-ecosystems. Exolaccase-boosted humification may be an admirable strategy for phenolic detoxification by creating multifunctional humic-like products (H-LPs). Nonetheless, the potential applicability of the formed H-LPs in agricultural production is still overlooked.

Development of an Orally Active Small-Molecule Inhibitor of Receptor Activator of Nuclear Factor-κB Ligand.

Receptor activator of nuclear factor-κB (RANK) and its ligand, RANKL, play pivotal roles in bone remodeling. The monoclonal antibody denosumab successfully inhibited the maturation of osteoclasts (OCs) by binding to RANKL in the clinic. We continued our efforts to develop small-molecule inhibitors of RANKL.

Inhibition mechanisms of Fe/Fe and Mn on fungal laccase-enabled bisphenol a polyreaction.

Bisphenol A (BPA) is regarded as an endocrine disruptor associated with negative health effects in animals and humans. Laccase from white-rot fungus can enable BPA oxidation and auto-polymerization to circumvent its biotoxicity, but the work concerning the effect mechanisms of divalent and trivalent metal ions (MIs) on BPA polyreaction have rarely been reported. Herein, Trametes versicolor laccase-started BPA conversion within 1 h followed pseudo-first order kinetics, and the rate constant (k) and half-life were respectively 0.

New insights into humic acid-boosted conversion of bisphenol A by laccase-activated co-polyreaction: Kinetics, products, and phytotoxicity.

How humic acid (HA) modifies bisphenol A (BPA) conversion in exoenzyme-activated polyreaction is poorly understood. Herein, the influencing mechanism of HA on laccase-induced BPA self-polymerization was investigated, and the phytotoxicity of the produced BPA self/co-polymers was assessed for the first time. HA prominently boosted BPA elimination, and the rate constants of BPA conversion augmented from 0.

The LCK-14-3-3ζ-TRPM8 axis regulates TRPM8 function/assembly and promotes pancreatic cancer malignancy.

Transient receptor potential melastatin 8 (TRPM8) functions as a Ca-permeable channel in the plasma membrane (PM). Dysfunction of TRPM8 is associated with human pancreatic cancer and several other diseases in clinical patients, but the underlying mechanisms are unclear. Here, we found that lymphocyte-specific protein tyrosine kinase (LCK) directly interacts with TRPM8 and potentiates TRPM8 phosphorylation at Y1022.

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting.

Traditional composting is a well-suited biotechnology for on-farm management of livestock manure (LM) but still leads to the release of toxic micropollutants and imbalance of nutrients. One exoenzyme-assisted composting has shown promise to ameliorate the agronomical quality of end products by improving humification and polymerization. The naturally occurring extracellular laccase from microorganisms belongs to a multicopper phenoloxidase, which is verified for its versatility to tackle micropollutants and conserve organics through the reactive radical-enabled decomposition and polymerization channels.

Suppression mechanism of model humic constituents on laccase-enabled 17β-estradiol oxidation and oligomerization.

Humic constituents (HCs) are ubiquitous in the aquatic ecosystems, and contain various functional groups that seriously impact the conversion of 17β-estradiol (17β-E2) by fungal laccase. The purpose of this study was to explore the influencing mechanism of HCs on Trametes versicolor laccase-enabled 17β-E2 oxidation and oligomerization. Herein, T.