Publications by authors named "Shunta Furuzawa"
Proc Natl Acad Sci U S A
August 2020
Article Synopsis
- Protein conformational changes during ligand binding are influenced by complex intra- and intermolecular events, with two main kinetic mechanisms: conformational selection (CS) and induced fit (IF).
- Experimental and computational methods were employed to analyze the coupled-folding/binding reaction of staphylococcal nuclease with adenosine-3',5'-diphosphate, revealing insights into specific protein-ligand interactions.
- The study found that the shift from CS to IF occurs due to stabilization of a compact transition state mainly through weak Coulombic interactions, altering the reaction pathway on the free energy surface depending on ligand concentration.
