Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regeneration.

Background: Bu-Shen-Huo-Xue (BSHX) decoction has been used in the postoperative rehabilitation of patients with spinal cord injury in China. In the present study, we aim to reveal the bioactive compounds in BSHX decoction and comprehensively explore the effects of BSHX decoction and the underlying mechanism in spinal cord injury recovery.

Methods: The main chemical constituents in BSHX decoction were determined by UPLC-MS/MS.

Sox11-modified mesenchymal stem cells accelerate cartilage defect repair in SD rats.

Cartilage has a limited capacity to heal. Previously, we have shown that overexpression of Sox11 in rMSCs (Rat Mesenchymal Stem Cells) by lentivirus-mediated gene transfer leads to enhanced tri-lineage differentiation and accelerated bone formation in fracture model of rats. We observed that the fracture repair in the rats that received Sox11-modified rMSCs injection proceeded through an endochondral ossification process much faster than those in the control groups.

Antibiotics Promote Escherichia coli-Pseudomonas aeruginosa Conjugation through Inhibiting Quorum Sensing.

The effect of antibiotics on horizontal gene transfer (HGT) is controversial, and the underlying mechanism remains poorly understood. Here, using SM10λπ as the donor strain, which carries a chromosomally integrated RP4 plasmid, we investigated the effect of antibiotics on conjugational transfer of a mobilizable gentamicin (Gm) resistance plasmid. The results showed that an exposure to gentamicin that restricted the survival of recipient cells significantly enhanced SM10λπ- PAO1 conjugation, which was attenuated by a deficiency of , genes associated with the generation of the quorum sensing signals -acyl homoserine lactones (AHLs) in PAO1, or the deletion of the AHL receptor SdiA in SM10λπ.

Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater.

A taxonomic study was performed on strain SYSU D3-2T, isolated from coastal seawater near the estuary of Pearl River in southern China. The strain was observed to be Gram-reaction-negative, non-motile and non-spore-forming. Cells were found to be of coccobacilli shape.

Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses - Conjugation through Inhibiting Expression.

Conjugation is a key mechanism for horizontal gene transfer and plays an important role in bacterial evolution, especially with respect to antibiotic resistance. However, little is known about the role of donor and recipient cells in regulation of conjugation. Here, using an (λπ)- () conjugation model, we demonstrated that deficiency of /, genes associated with generation of the quorum sensing signals N-acyl homoserine lactones (AHLs) in , or deletion of the AHLs receptor SdiA in the donor λπ both facilitated conjugation.

Profiling kidney microRNAs from juvenile grass carp (Ctenopharyngodon idella) after 56days of oral exposure to decabromodiphenyl ethane.

Grass carp (Ctenopharyngodon idella) is one of the most important species in China. Decabromodiphenyl ethane (DBDPE) is a brominated flame retardant that has been used widely in industry, and has been observed to accumulate in the tissues of fish from South China. Evidence has shown that DBDPE is toxic to aquatic animals, but the molecular response has been unclear.

Mechanism of azithromycin inhibition of HSL synthesis in Pseudomonas aeruginosa.

Pseudomonas aeruginosa is an opportunistic pathogen and a leading cause of nosocomial infections. Unfortunately, P. aeruginosa has low antibiotic susceptibility due to several chromosomally encoded antibiotic resistance genes.

Heat shock factor 1 is a transcription factor of Fas gene.

In mammalian cells, stress-induced expression of heat shock protein is controlled by heat shock factor 1 (HSF1). However, HSF1 functions as a regulator of additional genes. In this study, we observed that heat treatment effectively induced expression of Fas.

Dominant-positive HSF1 decreases alpha-synuclein level and alpha-synuclein-induced toxicity.

Alpha-synuclein aggregation and cytotoxicity are widely considered to play a critical role in the process of Parkinson's disease. Heat shock proteins are a large family of cellular protective molecules in most kinds of cells. In this study, we examined the impact of dominant-positive heat shock transcription factor 1 (HSF1) on alpha-synuclein over-expression cellular model of Parkinson's disease.

Induction of macroautophagy by heat.

Macroautophagy is a regulated bulk degradation process of cellular components, mainly long-lived proteins or cytoplasmic organelles. Nutrient depletion is a classic inducer of macroautophagy. In this report, we have induced heat-mediated macroautophagy in several cell lines in the absence of nutrient depletion.

[HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages].

Objective: To observe the effect of heat shock factor 1 (HSF1) on heat stress-induced apoptosis in Raw264.7 macrophages.

Methods: Raw264.

[Characteristics and expression of Mip5, a novel gene associated with myocardial ischemia/reperfusion in rats].

Objective: To determine the characteristics of a novel gene Mip5 (GenBank accession number AY553870) and its expression under physiological and pathological conditions.

Methods: The characteristics of Mip5 were analyzed by bioinformatic programs including BLAST, spidey, psort, ClustalW and so on. RT-PCR was performed to detect Mip5 expression.

[Effect of heat shock response on the cleavage of nucleolin induced by oxidative stress].

Objective: To observe the cleavage of nucleolin (C23) during apoptosis induced by oxidative stress and to clarify the effect of heat shock response (HSR) on the cleavage of nucleolin and its possible molecular mechanism.

Methods: We added 0.5 mmol/L peroxide hydrogen (H2O2 ) into cultured cells to mimic oxidative stress.

[Effect of nucleolin down-regulation on the proliferation and apoptosis in C2C12 cells].

Objective: To clarify the effect of nucleolin on the proliferation and apoptosis in C2C12 cells.

Methods: After inhibiting the expression of nucleolin using antisense oligonucleotides, the cellular proliferation was determined by MTT, and the apoptosis was detected by flow cytometry (FCM) assays and DNA ladder assays.

Results: After being transfected with antisense oligonucleotides for 24 hours, Western blotting showed that the expression of nucleolin was repressed significantly.