The clinical application of metagenomic next-generation sequencing in sepsis of immunocompromised patients.

Background: Metagenomic next-generation sequencing (mNGS) was commonly applied given its ability to identify and type all infections without depending upon culture and to retrieve all DNA with unbiasedness. In this study, we strive to compare outcomes of mNGS with conventional culture methods in adults with sepsis, investigate the differences between the immunocompromised and control group, and assess the clinical effects of mNGS.

Methods: In our study, 308 adult sepsis patients were included.

Risk Factors for Mortality in Sepsis Patients without Lactate Levels Increasing Early.

Objectives: Our purpose was to investigate the influencing factors for mortality in sepsis patients without lactate levels increasing in the early stage.

Methods: We conducted a retrospective observational study involving 830 adult sepsis patients admitted to ICU. We calculated time-weighted lactate (LacTW), a dynamic value that incorporates both the magnitude of change and the time interval of such change, to represent lactate levels in the first 24 hours.