Hopf bifurcation, stability switches and chaos in a prey-predator system with three stage structure and two time delays.

A three stage-structured prey-predator model with digestion delay and density dependent delay for the predator is investigated. The stability of the equilibrium point and the Hopf bifurcation of the system by choosing time delay as a bifurcation parameter in five cases are considered, and the conditions for the positive equilibrium occurring local Hopf bifurcation are given in each case. Numerical results show that delayed system considered has not only periodic oscillation, stability switches but also chaotic oscillation, even unbounded oscillation.

Deciphering transcriptome profile of the yellow catfish (Pelteobagrus fulvidraco) in response to Edwardsiella ictaluri.

Edwardsiella ictaluri is one of the most important pathogens posing a serious threat for yellow catfish (Pelteobagrus fulvidraco), a highly valuable fish species of increasing commercial interest in China. Here, a transcriptomic strategy was undertaken to investigate the yellow catfish gene expression profile against infection by the bacterial pathogen E. ictaluri.

De novo annotation of the immune-enriched transcriptome provides insights into immune system genes of Chinese sturgeon (Acipenser sinensis).

Chinese sturgeon (Acipenser sinensis), one of the oldest extant actinopterygian fishes with very high evolutionary, economical and conservation interest, is considered to be one of the critically endangered aquatic animals in China. Up to date, the immune system of this species remains largely undetermined with little sequence information publicly available. Herein, the first comprehensive transcriptome of immune tissues for Chinese sturgeon was characterized using Illumina deep sequencing.

[Fechtner syndrome, a nonmuscle myosin heavy chain 9 gene mutation related disease: a case report and literature review].

Objective: To improve the recognition of Fechtner syndrome.

Methods: The clinical and laboratory data and family survey of a patient with Fechtner's syndrom was reported.

Results And Conclusion: Giant platelets, thrombocytopenia and characteristic granulocyte inclusion bodies (Döhle-like bodies) were found in both peripheral blood and bone marrow smears of the patient.

[Enhancement of As-accumulation by Pteris vittata L. affected by microorganisms].

A pot experiment was carried out to study the root character and As accumulation of Pteris vittata L. affected by actinomycete PSQ, shf2 and bacteria Ts37, C13. The results indicated that growth of the fern was improved by the microorganisms.

Using protein design to dissect the effect of charged residues on metal binding and protein stability.

Ca2+ controls biological processes by interacting with proteins with different affinities, which are largely influenced by the electrostatic interaction from the local negatively charged ligand residues in the coordination sphere. We have developed a general strategy for rationally designing stable Ca2+- and Ln3+-binding proteins that retain the native folding of the host protein. Domain 1 of cluster differentiation 2 (CD2) is the host for the two designed proteins in this study.

Construction of a transposon-mediated baculovirus vector Hanpvid and a new cell line for expressing barnase.

In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1).

Design of a calcium-binding protein with desired structure in a cell adhesion molecule.

Ca2+, "a signal of life and death", controls numerous cellular processes through interactions with proteins. An effective approach to understanding the role of Ca2+ is the design of a Ca2+-binding protein with predicted structural and functional properties. To design de novo Ca2+-binding sites in proteins is challenging due to the high coordination numbers and the incorporation of charged ligand residues, in addition to Ca2+-induced conformational change.