Publications by authors named "Shun-mei E"
Article Synopsis
- Bacteria exposed to low doses of antibiotics, known as sub-minimal inhibitory concentrations (sub-MIC), can develop antibiotic resistance, but the exact mechanisms behind this are not fully understood.
- In a study, researchers used a type of E. coli to see how sub-MIC levels of antibiotics Ciprofloxacin or Levofloxacin affected the transfer of plasmids (small DNA molecules) to Pseudomonas aeruginosa.
- The results revealed that low doses of these antibiotics increased the transfer frequency of the plasmid, suggesting that sub-MIC conditions enhance gene expression related to plasmid transfer through mechanisms that do not rely on the bacterial SOS response.
Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater.
Int J Syst Evol Microbiol
July 2017
A taxonomic study was performed on strain SYSU D3-2T, isolated from coastal seawater near the estuary of Pearl River in southern China. The strain was observed to be Gram-reaction-negative, non-motile and non-spore-forming. Cells were found to be of coccobacilli shape.
View Article and Find Full Text PDF[Immortalization of embryonic fibroblasts in heat shock transcription factor 1 knockout mouse].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
April 2006
Article Synopsis
- - The study aimed to create immortalized embryonic fibroblast cell lines from both HSF1-deficient (HSF1-/-) and normal (HSF1+/+) mice to investigate the role of heat shock factor 1 (HSF1).
- - Researchers used a specific vector to introduce SV40 large T antigen into the fibroblasts and confirmed successful integration and expression of this gene in both cell lines via various techniques.
- - After six months of cultivation, the HSF1+/+ and HSF1-/- fibroblast lines displayed stable growth, but HSP70, a protein typically induced by heat shock, was not activated in the HSF1-/- cells.
[HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
April 2006
Objective: To observe the effect of heat shock factor 1 (HSF1) on heat stress-induced apoptosis in Raw264.7 macrophages.
Methods: Raw264.
[Characteristics and expression of Mip5, a novel gene associated with myocardial ischemia/reperfusion in rats].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2005
Objective: To determine the characteristics of a novel gene Mip5 (GenBank accession number AY553870) and its expression under physiological and pathological conditions.
Methods: The characteristics of Mip5 were analyzed by bioinformatic programs including BLAST, spidey, psort, ClustalW and so on. RT-PCR was performed to detect Mip5 expression.
[Effect of heat shock response on the cleavage of nucleolin induced by oxidative stress].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
October 2004
Objective: To observe the cleavage of nucleolin (C23) during apoptosis induced by oxidative stress and to clarify the effect of heat shock response (HSR) on the cleavage of nucleolin and its possible molecular mechanism.
Methods: We added 0.5 mmol/L peroxide hydrogen (H2O2 ) into cultured cells to mimic oxidative stress.
[Effect of nucleolin down-regulation on the proliferation and apoptosis in C2C12 cells].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
April 2005
Objective: To clarify the effect of nucleolin on the proliferation and apoptosis in C2C12 cells.
Methods: After inhibiting the expression of nucleolin using antisense oligonucleotides, the cellular proliferation was determined by MTT, and the apoptosis was detected by flow cytometry (FCM) assays and DNA ladder assays.
Results: After being transfected with antisense oligonucleotides for 24 hours, Western blotting showed that the expression of nucleolin was repressed significantly.