Epimedium sagittatum inhibits TLR4/MD-2 mediated NF-κB signaling pathway with anti-inflammatory activity.

Background: Epimedium sagittatum (Sieb.et Zucc.) Maxim.

[In vivo study of Chuankezhi metabolism in rat].

To study the metabolic products of main compounds of Chuankezhi injection in rat, 12 Sprague Dawley rats were classed into 2 groups, a blank control group and an intermuscular administration group, respectively. Rat feces and urine samples were collected from 0−24 h and 24−48 h after administration. All the samples were ultrasonically treated with methanol and then analyzed using LC-LTQ Orbitrap MSn.

[Simultaneous determination of 4 prenylflavonoids of Chuankezhi injection in rat plasma by LC-MS/MS].

A quantitative method for epimedin A, B, C and icariin in rat plasma was established using LC-MS/MS after intermuscular administration of Chuankezhi injection to rat. Chromatographic separation was performed on an Agilent Eclipse XDB-C(18) column (150 mm × 2.1 mm, 5.

[Pharmacokinetics of epimedin A, B, C and icariin of Chuankezhi injection in rat].

To study pharmacokinetic characteristics of epimedin A, B, C and icariin after intermuscular administration of Chuankezhi injection to rat. The established RRLC-MS/MS method was applied for simultaneous determination of four analytes in rat plasma and calculating their pharmacokinetic parameters. As a result, each analyte showed a good linear relationship in the concentration range of 1-1 000 μg•L⁻¹.

[Simultaneous determination of 4 phenolic acids in cangerzi by ultra-performance liquid chromatography].

In this study, an analytical method was developed and used to quantify simultaneously protocatechuic acid, neochlorogenic acid, cryptochlorogenic acid and 1, 3-dicaffeoylquinic acid--four bioactive compounds contained in Fructus Xanthii using UPLC. The contents of four phenolic components of 28 batches of samples collected from different product areas and markets were determined and compared by means of this established method. The mobile phase was composed of methanol and water containing 0.

Quantitative and fingerprint analyses of Chinese sweet tea plant ( Rubus suavissimus S. Lee).

The quality of botanical food is increasingly assessed by the content of multiple bioactive compounds. This study reports, for the first time, an HPLC fingerprinting method for the quality evaluation of Rubus suavissimus leaves possessing multiple bioactivities. Five constituents, gallic acid, rutin, ellagic acid, rubusoside, and steviol monoside, were quantified and used in developing qualitative chromatographic fingerprints.

Determination of ginsenoside-Rg(1) in human plasma and its application to pharmacokinetic studies following intravenous administration of 'Shenmai' injection.

'Shenmai' injection is derived from traditional Chinese medicine 'Shenmaisan' and made from Radix ginseng Rubra and Radix Ophiopogonis. Ginsenoside-Rg(1), as a major constituent of Radix ginseng Rubra, is considered responsible for the efficacy of this injection. A rapid, simple and accurate method has been established for determination of ginsenoside-Rg(1) in Shenmai injection and human plasma using LC-ESI-MS/MS, and to study the pharmacokinetics of Rg(1) in ten healthy volunteers after intravenous single dosing of 60 mL of Shenmai injection.

[HPLC fingerprinting of radix paeoniae alba].

To establish a sensitive and specific HPLC method for quality control of Radix Paeoniae Alba, HPLC method was applied for quality assessment of Radix Paeoniae Alba. HPLC analysis was performed on a Symmetry C18 column (250 mm x 4. 6 mm ID, 5 microm, Waters, USA).

[Studies on HPLC fingerprint of cortex moutan].

Objective: To establish a HPLC fingerprint for quality evaluation of Cortex Moutan.

Method: The HPLC chromatographic fingerprinting of 30 lots of Cortex Moutan was established and major peaks were identified by LC-MS and MS-MS.

Result: The HPLC fingerprint of Cortex Moutan was established, showing 15 characteristic peaks.

[Rapid identification of compounds in cortex moutan by liquid chromatography-tandem mass spectrometry].

Aim: To analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.

Methods: An on-line optimized HPLC-DAD/MS2 technique was employed.

Results: In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated.

Characterization of compounds in the Chinese herbal drug Mu-Dan-Pi by liquid chromatography coupled to electrospray ionization mass spectrometry.

Cortex Moutan is a well-known traditional Chinese medicine derived from Paeonia suffruticosa ANDREWS. However, root cortices of P. delavayi and P.

[Determination of ginsenoside Rd and its metabolites in rat urine by LC-MS].

Aim: To study the metabolic pathways of ginsenoside Rd in rats.

Methods: Urine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg of ginsenoside Rd to six rats, separately. The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for putative metabolites.

[Establishment of a method to detect multiple antennae carcinoembryonic antigen (MA-CEA) and MA-CEA level in the sera of patients with malignant tumors].

Aim: To establish a rapid quantitative method to detect the serum multiple antennae carcinoembryonic antigen (MA-CEA), and to measure the MA-CEA levels in the sera of patients with malignant tumors.

Methods: Neuraminidase (NMD) was used to digest the sialic acid at terminals of sugar chains of MA-CEA, and then the datura stramonium agglutinin (DSA) and anti-CEA antibody were used to set up streptavidin-biotin complex (ABC) system EIA (ABC-EIA) for detecting serum MA-CEA levels of 239 patients with carcinomas of lung, stomach, liver, colon and ovary. The serum CEA level was determined by ELISA.