[Contributions of impaired adult hippocampal neurogenesis to occurrence and development of diabetic encephalopathy].

Diabetic encephalopathy (DE) is one of the most common complications of diabetes mellitus (DM). Persistent hyperglycemia in DM patients may induce numerous pathophysiological changes, such as chronic inflammation, increased permeability of blood-brain barrier, impaired neurogenesis, and brain atrophy, which eventually impair cognitive function. The dentate gyrus (DG) of hippocampus is a crucial region for learning and memory, as well as adult neurogenesis in mammals.

Prevalence and risks of mild cognitive impairment of Chinese community-dwelling women aged above 60 years: A cross-sectional study.

Evidence has shown that risks of cognitive impairment differ between genders. This cross-sectional study sought to determine the prevalence of mild cognitive impairment (MCI) in Chinese community-dwelling women aged above 60 years and identify risks of MCI by multivariate logistic regression analysis. Totally, 1760 Chinese community-dwelling women entered the study.

[Research progress on the epigenetic mechanisms of inverse relationship between Alzheimer's disease and cancer onset].

Alzheimer's disease (AD) is currently the most prevalent neurodegenerative disease in the aging population. It is characterized by massive deposition of extracellular β-amyloid peptide and formation of intracellular neurofibrillary tangles. Cancer is also an age-related disease.

[Influence of high-voltage electrical burn on the rheological property of platelet and leukocyte in rats and the interventional effect of pentoxifylline].

Objective: To investigate the influence of high-voltage electrical burn (HEB) on the aggregation and adhesion of platelet and leukocyte in rats and the interventional effect of pentoxifylline (PTX).

Methods: One hundred and eighty SD rats were divided into control, electrical burn (EB), and pentoxifylline treatment (PT) groups according to the random number table, with 60 rats in each group. (1) Ten rats were taken from each group at 15 minutes before injury for the observation of the microcirculatory perfusion of chest skin with Laser Doppler Perfusion Imager (LDPI), and the number of leukocyte adherent to mesenteric venule with Bradford Variable Projection Microscope (BVPM).

[Clinical effect of the concentrated suture fixation method on skin transplantation in the jaw and neck region].

Objective: To observe the clinical effect of the concentrated suture fixation method on skin transplantation on deep burn wound or wound of cicatricial deformity after burn in the jaw and neck region.

Methods: One hundred and fourteen patients, hospitalized from April 2002 to December 2011, with deep burn or cicatricial deformity after burn in the jaw and neck region, were divided into packaging group and concentrated suture group according to the random number table. Each group had 57 patients including 48 cases with deep burn and 9 cases with cicatricial deformity.

[Activation mechanisms of protein kinase Czeta and its cellular function].

The zeta isotype of protein kinase C (PKCzeta) is a member of the atypical PKC subfamily and has been widely implicated in the regulation of cellular functions. PKCzeta, as an important message molecular, is involved in many intracellular signaling pathways, such as the mitogen-avtivated protein kinase (MAPK) cascade, transcriptional factor NF-kappaB activation, ribosomal S6-protein kinase signaling and so on. Increasing evidence from studies support the concept that the activation mechanisms of PKCzeta are tissue/cell specific.

Effect of cholecystokinin octapeptide on diacylglycerol-PKC signaling pathway in rat pulmonary interstitial macrophages stimulated by lipopolysaccharide.

Aim: To investigate the effect of cholecystokinin octapeptide (CCK-8) on the diacylglycerol-protein kinase C (DAG-PKC) signaling pathway in rat pulmonary interstitial macrophages (PIM) stimulated by lipopolysaccaride (LPS).

Methods: The PIM from rat lung tissues were isolated using the collagenase digestion method combined with alveolar lavage and pulmonary vessel perfusion. DAG content and PKC activity were measured by radioenzymatic assay.

Effect of lipopolysaccharide on expression and characterization of cholecystokinin receptors in rat pulmonary interstitial macrophages.

Aim: To investigate the effect of lipopolysaccharide (LPS) on the expression and the binding characteristics of cholecystokinin receptors (CCK-R) in rat pulmonary interstitial macrophages (PIMs).

Methods: The PIMs isolated from rat lung tissues were purified by the collagenase digestion method combined with alveolar lavage and pulmonary vessel perfusion. The expression of CCK-R mRNA was detected by RT-PCR and Southern blot analysis and the binding experiments were performed by radioligand binding assay (RBA).

[Specific anti-tumor effect of immune competent cell induced by HSP-70 of HeLa cells in vitro].

Background & Objective: Recent studies indicated that heat shock proteins (HSPs) are associated with antigenic peptides and participate in the antitumor T cell response in animals. This study was designed to investigate the mechanism of anti-tumor effect of immune competent cells (ICC) induced by HSP-70 of HeLa (cervical carcinoma) cells in vitro.

Methods: The normal peripheral blood mononuclear cells(PBMCs) stimulated with HSP-70 of HeLa cells in vitro.