Lanthanide Organic Framework as a Reversible Luminescent Sensor for Sulfamethazine Antibiotics.

A water-stable two-dimensional lanthanide organic framework, {Eu(BTB)DMF} (Eu-MOF; DMF = N, N-dimethylformamide), with two one-dimensional channels was obtained, and its structure was characterized. With changes in the amount of LiOH·HO, different sizes of {Eu(BTB)DMF} were synthesized. The prepared Eu-MOF powder is easy to disperse in water and exhibits typical Eu red emission.

[Screening and cloning of hepatitis C virus non-structural protein 4B interacting protein gene in hepatocytes].

Objective: To investigate biological functions of non-structural protein 4B (NS4B) of hepatitis C virus (HCV), yeast-two hybrid technique was performed to seek proteins in hepatocytes interacting with HCV NS4B.

Methods: HCV NS4B bait plasmid was constructed by ligating the NS4B gene with carrier plasmid pGBKT7 and transformed into yeast cells AH109 (type alpha). The transformed yeast cells were amplified and mated with yeast cells Y187 (alpha type) containing liver cDNA library plasmid pACT2 in 2 x YPDA medium.

[Screening and cloning of hepatitis C virus non-structural protein 4A interacting protein gene in hepatocytes].

Objective: To investigate biological functions of hepatitis C virus (HCV) non-structural protein 4A (NS4A).

Methods: Yeast-two hybrid technique was performed to seek proteins in hepatocytes interacting with HCV NS4A. HCV NS4A bait plasmid was constructed by ligating the NS4A gene with carrier plasmid pGBKT7, then it was transformed into yeast AH109 (alpha type).

[Screening and cloning target genes transactivated by hepatitis C virus F protein using suppression subtractive hybridization technique].

Objectives: To identify and clone human genes transactivated by HCV F protein by constructing a cDNA subtractive library using the suppression subtractive hybridization technique.

Methods: Suppression subtractive hybridization (SSH) and bioinformatics techniques were used for screening and cloning of the target genes transactivated by HCV F protein. The mRNA was isolated from HepG2 cells transfected with pcDNA3.