Postbiotic studies of mixed cultures of and B-5P produced b rumen producing short-chain fatty acid.

Background And Aim: Postbiotics are functional bioactive compounds or bioactive molecules with beneficial effects on health and functional activities in humans or livestock, produced by probiotic bacteria or yeast. Several postbiotics, including enzymes, short-chain fatty acids, amino acids, extracellular polysaccharides, microbial cell fragments, and teichoic acids, are currently being widely studied. This study aimed to explore the potential of secondary metabolites of and as lactic acid bacteria (LAB) and yeast isolated from Budu (fermented fish) which can act as postbiotics through rumen fermentation.

Screening of different growth conditions of isolated from membrane-less microbial fuel cell toward antimicrobial activity profiling.

Bacteriocins produced by have gained recognition for their safe use in humans. In this study, we aimed to assess the inhibitory activity of an antimicrobial peptide synthesized by the wild-type strain of against the notorious pathogen . Our investigation employed the broth microdilution method to evaluate the inhibitory potential of this peptide.

SEPTIN2 suppresses an IFN-γ-independent, proinflammatory macrophage activation pathway.

Interferon-gamma (IFN-γ) signaling is necessary for the proinflammatory activation of macrophages but IFN-γ-independent pathways, for which the initiating stimuli and downstream mechanisms are lesser known, also contribute. Here we identify, by high-content screening, SEPTIN2 (SEPT2) as a negative regulation of IFN-γ-independent macrophage autoactivation. Mechanistically, endoplasmic reticulum (ER) stress induces the expression of SEPT2, which balances the competition between acetylation and ubiquitination of heat shock protein 5 at position Lysine 327, thereby alleviating ER stress and constraining M1-like polarization and proinflammatory cytokine release.

sp. nov. and sp. nov., halophilic archaea isolated from sediment of a marine saltern and inland saline soil.

Two halophilic archaeal strains, ZS-10 and GSL13, were isolated from the Zhoushan marine saltern in Zhejiang, and an inland saline soil from the Tarim Basin, Xinjiang, PR China, respectively. The cells of strain ZS-10 were pleomorphic while those of strain GSL13 were rod-shaped. Both of them stained Gram-negative and formed red-pigmented colonies on agar plates and their cells lysed in distilled water.

sp. nov. and sp. nov., extremely halophilic archaea isolated from salted brown alga and coastal saline-alkali lands.

Four extremely halophilic archaeal strains, LYG-108, LYG-24, DT1 and YSSS71, were isolated from salted produced in Lianyungang and saline soil from the coastal beach at Jiangsu, PR China. The four strains were found to be related to the current species of (showing 88.1-98.

Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., Natrinema zhouii sp. nov., extremely halophilic archaea isolated from marine environments and a salt mine.

Four extremely halophilic archaeal strains (ZJ2, BND6, DT87, and YPL30) were isolated from marine environments and a salt mine in China. The 16S rRNA and rpoB' gene sequence similarities among strains ZJ2, BND6, DT87, YPL30 and the current species of Natrinema were 93.2-99.

sp. nov. and sp. nov., two extremely halophilic archaea isolated from Gobi saline soil.

Two extremely halophilic archaeal strains, PSR5 and PSR8, were isolated from a saline soil sample collected from the Tarim Basin, Xinjiang, PR China. Both strains had two copies of the 16S rRNA genes and , showing 2.6 and 3.

Evolution of water environment construction and urban landscape ecological risk based on land cover change analysis.

Mining minerals plays a fundamental role in promoting social-economic progress. Intensive mining operations. However, it also brings huge ecological risks, and may lead to significant changes in land use/land cover (LULC), further affecting the urban landscape and ecological environment.

Symbiotic treatment of ammonia-nitrogen wastewater by algae and activated sludge: effects of algae and sludge inoculation rates.

A symbiotic microalgal-bacterial system may be an optional technology for wastewater treatment. It was composed of microalgae and activated sludge and established in the SBR to explore the effect of different dosing ratios of algae and sludge on the removal of nitrogen and phosphorus from simulated wastewater containing ammonium. It can be seen from the result that varied algae-sludge dosing ratios had a higher removal effect on COD removal, but the difference was not significant.

SARS-CoV-2 Quasispecies Provides an Advantage Mutation Pool for the Epidemic Variants.

The dynamics of quasispecies afford RNA viruses a great fitness on cell tropism and host range. To study the quasispecies features and the intra-host evolution of SARS-CoV-2, we collected nine confirmed patients and sequenced the haplotypes of spike gene using a single-molecule real-time platform. Fourteen samples were extracted from sputum, nasopharyngeal swabs, or stool, which in total produced 283,655 high-quality circular consensus sequences.

MiR-342 controls Mycobacterium tuberculosis susceptibility by modulating inflammation and cell death.

Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) that places a heavy strain on public health. Host susceptibility to Mtb is modulated by macrophages, which regulate the balance between cell apoptosis and necrosis. However, the role of molecular switches that modulate apoptosis and necrosis during Mtb infection remains unclear.

Comparison of Retrospective and Prospective Falls Reporting Among Community-Dwelling Older People: Findings From Two Cohort Studies.

While prospective recording is considered as the gold standard, retrospective recall is widely utilized for falls outcomes due to its convenience. This brings about the concern on the validity of falls reporting in Southeast Asian countries, as the reliability of falls recall has not previously been studied. This study aimed to evaluate the reliability of retrospective falls recall compared to prospective falls recording.

Effect of SARS-CoV-2 coinfection was not apparent on the dynamics of chronic hepatitis B infection.

In patients coinfected with SARS-CoV-2 and HBV, liver injury was common. However, the interactions between SARS-CoV-2 and HBV coinfection remained unknown. Sixty-seven COVID-19 patients from the previous cohort were enrolled and classified into 2 groups (7 with HBsAg+ and 60 with HBsAg-).

Overexpression of Wnt7a enhances radiosensitivity of non-small-cell lung cancer via the Wnt/JNK pathway.

The Wingless-type protein 7a (Wnt7a) plays an antiproliferative role in non-small-cell lung cancer (NSCLC). Previous studies have indicated that Wnt7a expression was downregulated in radiation-resistant NSCLC cells. However, little is known about its biological functions and molecular mechanisms in radiosensitivity of NSCLC.