Transgenesis of the gonochoristic nematode by microparticle bombardment with hygromycin B selection.

The gonochoristic nematode is the phylogenetically closest species to the well-studied nematode (Kanzaki , 2018). While has been expected to be a useful comparative model for , efficient transgenesis methods have not been available. Here, we established a method to integrate transgenes into the genome by microparticle bombardment with hygromycin B selection.