A novel NIR fluorescent probe for copper(ii) imaging in Parkinson's disease mouse brain.

Abnormal copper ion (Cu) levels are considered to be one of the pathological factors of Parkinson's disease (PD), but the internal relationship between Cu and PD progression remains elusive. Visualizing Cu in the brain will be pivotal for comprehending the underlying pathophysiological processes of PD. In this work, a near-infrared (NIR) fluorescent probe, DDAO-Cu, capable of detecting Cu with exceptional sensitivity (about 1.

Cell-penetrating peptides noncovalently modified red phosphorescent nanoparticles for high-efficiency imaging.

The application of long-lived phosphorescence probes in time-resolved luminescence imaging is limited by their low quantum yield in aqueous solutions. However, sensitization of thermally activated delayed fluorescence (TADF) materials can compensate for this limitation while addressing the issue of insufficient proportion of their own long lifetime. In this study, we utilized the characteristics of phosphorescence and TADF materials simultaneously by doping the receptor iridium complex PMD-Ir into the donor TADF polymer PCzDP-20 through donor-receptor doping method, and successfully prepared highly efficient red phosphorescent nanoparticles.

Effects of lane-change scenarios on lane-change decision and eye movement.

Improper lane-change manoeuvre can cause traffic safety issues and even lead to serious traffic collisions. Quantifying the decision behaviour and eye movements can provide a deeper understanding of lane-change manoeuvre in vehicle interaction environment. The purpose of this study was to investigate the effect of lane-change scenarios defined by gaps on lane-change decision and eye movements.

Dual-Channel Fluorescent Probe for Detecting Viscosity and ONOO without Signal Crosstalk in Nonalcoholic Fatty Liver.

Nonalcoholic fatty liver disease (NAFLD) is a global health issue. Peroxynitrite and liver viscosity have recently been found to be potential biomarkers of NAFLD. Therefore, it is of great significance to develop dual-response fluorescent probes for simultaneous detecting peroxynitrite and viscosity.

Polarity-Sensitive Cell Membrane Probe Reveals Lower Polarity of Tumor Cell Membrane and Its Application for Tumor Diagnosis.

Cancer is a health threat worldwide, and it is urgent to develop more sensitive cancer detection methods. Herein, a polarity-sensitive cell membrane probe (named ) was developed for detecting cancer cells and tumors sensitively and selectively at the cell membrane level. The probe shows a strong polarity-dependent fluorescence and excellent cell membrane targeting ability to visualize cell membrane with red fluorescence with a non-washing process.

Two Water-Soluble and Wash-Free Fluorogenic Probes for Specific Lighting Up Cancer Cell Membranes and Tumors.

The construction of microenvironment-sensitive probes with good cell membrane-targetability can reveal the fundamental properties of cell membranes. Herein, two polarity-sensitive probes, termed were reported for the first time to specifically light up cancer cell membranes. Both probes were designed with tetrahydroquinoxaline coumarin amide as the fluorophore, and quaternary ammonium groups were appended to increase water solubility and target cell membranes.

Impacts of COVID-19 on urban rail transit ridership using the Synthetic Control Method.

The outbreak of COVID-19 in 2020 has had drastic impacts on urban economies and activities, with transit systems around the world witnessing an unprecedented decline in ridership. This paper attempts to estimate the effect of COVID-19 on the daily ridership of urban rail transit (URT) using the Synthetic Control Method (SCM). Six variables are selected as the predictors, among which four variables unaffected by the pandemic are employed.

Near-Infrared Mitochondria-Targetable Fluorescent Probe for High-Contrast Bioimaging of HS.

To elucidate the complex role of biological HS and study the mitochondrial damage and some related diseases, effective methods for visualization of HS in mitochondria and in vivo are urgently needed. In this contribution, a novel near-infrared mitochondria-targetable fluorescence probe for HS detection was developed. shows rapid detection ability for HS in pure aqueous solution and outputs a highly selective and sensitive fluorescence-on signal at 663 nm with a large Stokes shift of 141 nm.

Real-time tracking lysosomal pH changes under heatstroke and redox stress with a novel near-infrared emissive probe.

Lysosomes are important subcellular organelles with acidic pH. The change of lysosomal pH can affect the normal function and activity of cells. To conveniently detect and visualize lysosomal pH changes, we designed herein a novel fluorescent probe NIR-Rh-LysopH.

A novel reaction-based fluorescence probe for rapid imaging of HClO in live cells, animals, and injured liver tissues.

Hypochlorous acid (HClO) is an important bioactive molecule, playing vital roles in a large number of physiological and pathological processes. Abnormal concentration of HClO in vivo has close contact with many diseases including inflammatory diseases and cancer. For bioimaging HClO, a new colorimetric and turn-on near-infrared (NIR) fluorescence probe (DDAO-ClO) was designed and synthesized in this work through a specific reaction of HClO with dimethylthiocarbamate.

Aggregation-induced emission and solid fluorescence of fluorescein derivatives.

We report herein for the first time that the aggregation-induced emission and strong solid fluorescence of fluorescein derivatives can be realized by slightly modifying their structure, which provides a new option for AIEgens and solid fluorescent materials.

CO release with ratiometric fluorescence changes: a promising visible-light-triggered metal-free CO-releasing molecule.

The first visible-light-triggered metal-free and ratiometric fluorescent CORM is reported. This CORM can be used to release CO with distinct ratiometric fluorescence changes in aqueous solution, living cells, zebrafish, and mice, which provided an excellent controllable and trackable CORM for living systems.

A Fluorescent ESIPT Probe for Imaging CO-Releasing Molecule-3 in Living Systems.

CO-releasing molecule-3 (CORM-3) has been widely used recently as a convenient and safe CO donor to release exogenous CO in living cells and to study the effects of CO on cellular systems. Accordingly, development of effective methods for detecting and tracking CORM-3 in living systems is of great significance. In this work, a readily available fluorescent probe for detection of CORM-3 was reported for the first time.

A dual-channel probe with green and near-infrared fluorescence changes for in vitro and in vivo detection of peroxynitrite.

In this study, a coumarin-semirhodamine hybrid based dual-channel fluorescent probe was developed for detection of peroxynitrite. This probe works well in nearly pure aqueous solution and displays a rapid, highly sensitive and selective response for peroxynitrite at two different emission wavelengths (700 and 515 nm, respectively), along with distinct color and green to near-infrared fluorescence changes. The fluorescent intensity ratio at 700 and 515 nm increases linearly with the concentration of peroxynitrite from 0 to 50 μM, and the detection limit is estimated to be 59 nM.

Allyl Fluorescein Ethers as Promising Fluorescent Probes for Carbon Monoxide Imaging in Living Cells.

Recently, the fluorescent detection of carbon monoxide (CO) in living cells has attracted great attention. However, due to the lack of effective ways to construct fluorescent CO probes, fluorescent detection of CO in living cells is still in its infancy. In this paper, we report for the first time the use of allyl ether as a reaction site for construction of fluorescent CO probes.

Readily Available Fluorescent Probe for Carbon Monoxide Imaging in Living Cells.

Carbon monoxide (CO) is an important gasotransmitter in living systems and its fluorescent detection is of particular interest. However, fluorescent detection of CO in living cells is still challenging due to lack of effective probes. In this paper, a readily available fluorescein-based fluorescent probe was developed for rapid detection of CO.

Analysis of bus passenger comfort perception based on passenger load factor and in-vehicle time.

Although bus comfort is a crucial indicator of service quality, existing studies tend to focus on passenger load and ignore in-vehicle time, which can also affect passengers' comfort perception. Therefore, by conducting surveys, this study examines passengers' comfort perception while accounting for both factors. Then, using the survey data, it performs a two-way analysis of variance and shows that both in-vehicle time and passenger load significantly affect passenger comfort.

Risk factors affecting fatal bus accident severity: Their impact on different types of bus drivers.

While the bus is generally considered to be a relatively safe means of transportation, the property losses and casualties caused by bus accidents, especially fatal ones, are far from negligible. The reasons for a driver to incur fatalities are different in each case, and it is essential to discover the underlying risk factors of bus fatality severity for different types of drivers in order to improve bus safety. The current study investigates the underlying risk factors of fatal bus accident severity to different types of drivers in the U.