Publications by authors named "Shulin Ruan"
Introduction: The Vel- phenotype is a rare blood group, and it is challenging for identifying this phenotype due to limited available reagents. Moreover, there are relatively few studies on genomic editing of erythroid antigens and generation of knockout (KO) cell lines at present.
Methods: To identify the high-efficiency small-guiding RNA (sgRNA) sequence, candidate sgRNAs were transfected into HEK 293T cells and analyzed using Sanger sequencing.
Article Synopsis
- * Researchers identified a novel homozygous splice-site mutation (JK*02N) in the JK gene that resulted in exon skipping, confirming the mutation's role in the Jk(a-b-) phenotype.
- * The findings highlight the usefulness of minigene splicing assays for functional verification, but also point out differences when compared to cDNA sequencing methods.
Background: Epigenome-wide association studies (EWAS), which seek the association between epigenetic marks and an outcome or exposure, involve multiple hypothesis testing. False discovery rate (FDR) control has been widely used for multiple testing correction. However, traditional FDR control methods do not use auxiliary covariates, and they could be less powerful if the covariates could inform the likelihood of the null hypothesis.
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