Oncogenic RAS induces a distinctive form of non-canonical autophagy mediated by the P38-ULK1-PI4KB axis.

Cancer cells with RAS mutations exhibit enhanced autophagy, essential for their proliferation and survival, making it a potential target for therapeutic intervention. However, the regulatory differences between RAS-induced autophagy and physiological autophagy remain poorly understood, complicating the development of cancer-specific anti-autophagy treatments. In this study, we identified a form of non-canonical autophagy induced by oncogenic KRAS expression, termed RAS-induced non-canonical autophagy via ATG8ylation (RINCAA).

What are the symptom trajectories of self-regulatory fatigue among family caregivers of stroke survivors? A protocol of mixed-methods study in Chinese rehabilitation settings.

Introduction: Stroke presents a considerable burden not only to patients but also to their families and society at large. In many instances, stroke patients opt for home rehabilitation, relying on family caregivers for daily assistance. This dynamic significantly influences the physiological, psychological and social well-being of these caregivers.

Near-Infrared Phosphorescent Silver Nanoclusters/Polyethylenimine Nanocomposites for Photothermal Conversion.

Emerging as a promising functional material, metal nanoclusters that emit near-infrared (NIR) radiation have garnered significant attention due to their distinctive properties. Nonetheless, the rational design of NIR-emissive metal nanoclusters still faces substantial challenges. Herein, we demonstrate a self-assembly strategy for constructing NIR-emissive nanocomposites (abbreviated as ) using water-soluble Ag-NCs (Ag(mba), where Hmba = 2-mercaptobenzoic acid) and branched polyethylenimine (PEI) ( = 750,000).

A distinctive form of autophagy induced by oncogenic RAS.

RAS mutations enhance macroautophagy/autophagy in tumor cells, crucial for their growth and survival, making autophagy a promising therapeutic target for RAS-mutant cancers. However, the distinction between RAS-induced autophagy and physiological autophagy is not well understood. We recently identified a unique form of autophagy, RAS-induced non-canonical autophagy via ATG8ylation (RINCAA), which differs from starvation-induced autophagy.

Relationship between TyG-related index and hearing loss in people over 45 s in China.

Background: Triglycerides and fasting glycemic index are biomarkers used to assess the risk of insulin resistance and metabolic syndrome. It and its derivatives such as the TyG-BMI index are currently able to reflect the degree of insulin resistance and are closely related to a variety of metabolic diseases. The relationship between the TyG-BMI index and hearing loss remains underexplored, particularly in the context of aging and public health.

A Porphyrin-Based Metal-Organic Framework Nanozyme with Superior Peroxidase-like Activity for Combating Antibacterial Infections and Promoting Wound Healing.

As an antibacterial agent, HO is widely used to combat pathogenic bacterial infections clinically. To mitigate potential side effects associated with a high dosage of HO, it is pivotal to improve its antibacterial efficacy. Herein, a nanoscale porphyrin-based mesoporous metal-organic framework (MOF) nanozyme, Nano-PCN-222(Fe), was readily prepared by a one pot.

SRT3025-loaded cell membrane hybrid liposomes (3025@ML) enhanced anti-tumor activity of Oxaliplatin via inhibiting pyruvate kinase M2 and fatty acid synthase.

Background: Bladder cancer is one of the most common malignancies of the urinary system. Despite significant advances in diagnosis and treatment, the compromised therapeutic effect of chemotherapeutic agents, such as Oxaliplatin (OXA), remains a major clinical challenge. Thus, a combination therapy is required to enhance the OXA's therapeutic effectiveness and improve patient outcomes.

LncRNA TUG1 mitigates chronic kidney disease through miR-542-3p/HIF-1α/VEGF axis.

Renal interstitial fibrosis (RIF) is a common pathway in chronic kidney disease (CKD) that ultimately leads to end-stage renal failure, worsening both glomerulosclerosis and interstitial fibrosis. Ten percent of the adult population in the world suffers from CKD, and as the ageing population continues to rise, it is increasingly regarded as a global threat-a silent epidemic. CKD has been discovered to be closely associated with both long noncoding RNAs (lncRNAs) and microRNAs (miRNAs), while the precise molecular processes behind this relationship are still unclear.

Research on the application effect of arthroscopic access modification in meniscal injury repair.

Objective: To investigate the application value of arthroscopic channel modification in meniscal injury repair.

Methods: We retrospectively analyzed the data of 100 patients with meniscus injuries treated with knee arthroscopy from December 2022 to December 2023 and divided them into a control group and a modified group according to the application of "arthroscopic access modification technology". We compared the operation time, postoperative hospitalization time, VAS score, Lysholm knee function score, postoperative complications, and postoperative images of the patients in these two groups.

Comparative analyses of odorant binding protein orthologues in three sympatric Tomicus bark beetles provide insights into functional differentiation of OBPs to ecologically relevant odorants and insecticides.

Three Tomicus bark beetles (T. yunnanensis, T. brevipilosus and T.

Preparation and Evaluation of a Novel Branched Polymer as Thickener for Calcium Chloride-Based Drilling and Completion Fluids.

Calcium halide-based fluids are often used in drilling and completion operations due to their high density, clay inhibition and low solid content. However, there is a lack of thickeners to promote gel strength, which improves the fluid's capacity to carry and suspend cuttings. To solve this problem, the branched polymer (hereafter abbreviated as PAD-B) was prepared by the copolymerization of N,N-dimethylacrylamide (DMAM) and 2-acrylamide-2-methylpropane sulfonic acid (AMPS), using polyethylenimine as a branching agent and cerium ammonium nitrate as the initiator.

Functional characterization of four antenna-enriched odorant binding proteins in Rhaphuma horsfieldi reveals the importance of RhorOBP1 in odorant recognition and insecticide resistance.

The cerambycid beetles are key players for the sustenance of biodiversity in the forest ecosystem, but in most cases are well known due to their harmfulness to agricultural and forest plants. Here, we characterized the odorant binding protein (OBP) gene family in Rhaphuma horsfieldi, emphasizing the roles of RhorOBP1 in odorant reception and insecticide sequestering. A homology-based search led to the identification of 35 RhorOBP genes with a major distribution in the Minus-C OBPs clade (21/35 genes).

Collagen-disruptive cell therapy: adoptive transfer of membrane-anchored, tumor cell surface vimentin-targeted interleukin 12-armed TILs suppress collagen expression to boost deep T-cell infiltration via dual signaling activation and significant CCKAR reduction.

Tumor-targeted T-cell therapies of various types have been booming, but T-cell therapy is limited by its inability to penetrate the collagen barrier surrounding tumors. The destruction of tumor collagen is significant because collagen both suppresses T cells and contributes to the formation of the extracellular matrix. Our previously reported cell surface vimentin (CSV)-targeted and membrane-anchored IL12-armed (attIL12) T cells can reduce collagen production by killing cancer-associated fibroblasts, thereby increasing T-cell infiltration.

Association between sleep and tinnitus in US adults: Data from the NHANES (2007-2012).

We evaluated the relationship between sleep factors and tinnitus risk using data from the US National Health and Nutrition Examination Survey (2007-2012), focusing on adults aged 20 to 80 years. Our analysis included 4354 participants, with sleep patterns categorized as healthy, intermediate, or poor. We observed that both short (odds ratio [OR]: 1.

Supramolecular Luminescent Copper-Nanocluster-Based Dough with Excellent Electrical Conductivity Sensing Properties.

In recent years, the rapid advancement of flexible conductive materials has significantly increased the demand for dough materials that offer high flexibility and conductivity for diverse applications. Here, we developed a flexible, stretchable, and self-healing dough utilizing hydrogen-bonding interactions between glutathione-stabilized copper nanoclusters (GSH-Cu NCs) and poly(acrylic acid) (PAA). The dough materials can be kneaded, readily reshaped, and further processed to create bulk materials of arbitrary form factors.

Heterogeneous subgroups of psychological, physical and social condition and their predictors in Chinese older patients with coronary heart disease after stent implantation.

Objectives: To explore heterogeneous subgroups of the overall health condition in the older coronary heart disease patients with stent implantation and identify their predictors.

Methods: A cross-sectional study was surveyed in Hunan, China. Participants completed the general information questionnaire and PROMIS Profile-57.

Biomolecular condensates mediate bending and scission of endosome membranes.

Multivesicular bodies are key endosomal compartments implicated in cellular quality control through their degradation of membrane-bound cargo proteins. The ATP-consuming ESCRT protein machinery mediates the capture and engulfment of membrane-bound cargo proteins through invagination and scission of multivesicular-body membranes to form intraluminal vesicles. Here we report that the plant ESCRT component FREE1 forms liquid-like condensates that associate with membranes to drive intraluminal vesicle formation.

Fucoxanthin Attenuates Myocardial Ischemia/Reperfusion-Induced Injury via AMPK/GSK-3β/Nrf2 Axis.

Fucoxanthin (Fx), a xanthophyll carotenoid abundant in brown algae, possesses several biological functions, such as antioxidant, anti-inflammatory, and cardiac-protective activities. However, the role of Fx in myocardial ischemia/reperfusion (MI/R) is still unclear. Thus, the aim of this study was to investigate the effect of Fx on MI/R-induced injury and explore the underlying mechanisms.

Ubiquitin-like 4A alleviates the progression of intracerebral hemorrhage by regulating oxidative stress and mitochondrial damage.

Acupuncture has obvious therapeutic effect on intracerebral hemorrhage (ICH). miR-34a-5p regulated by acupuncture was found to attenuate neurological deficits in ICH. However, the underlying mechanisms are unclear.