[Association between angiotensin-converting enzyme and polymorphisms of N5, N10-methylenetetrahydrofolic acid reductase gene in patients with ischemic stroke].

Objective: To explore the association between angiotensin-converting enzyme (ACE) and the polymorphisms of N5, N10-methylenetetrahydrofolic acid reductase (MTHFR) gene in patients with ischemic stroke (IS).

Methods: Totally 454 patients with IS (IS group) and 334 controls (control group) were recruited in our study. Their I/D polymorphisms of ACE gene and C677T polymorphisms of MTHFR gene were detected by PCR and denaturing high performance liquid chromatography.

[Relationship between angiotensin converting enzyme gene and ischemic stroke].

Objective: To study relationship between angiotensin converting enzyme (ACE) gene and ischemic stroke (IS).

Methods: (1)Four hundred and fifty-four patients and 334 controls were recruited in our study, their I/D polymorphisms of ACE gene were detected by polymerase chain reaction (PCR) and denaturing high performance liquid chromatogram, and their risk factors of IS were recorded at the same time. (2)In addition, 29 stroke-prone spontaneously hypertensive rats (SHR-SP) and 40 Sprague-Dawley (SD) rats were enrolled, and hypoxia- apnoea animal models and simple apnoea animal models were used at the same time.

[Relationship between methylenetrahydrofolate reductase gene and ischemic stroke].

Objective: To study the relationship between methylenetrahydrofolate reductase (MTHFR) gene and ischemic stroke.

Methods: Four hundred and fifty four ischemic stroke patients were enrolled in the study. They were divided into large artery atherosclerosis (LAA), cardioembolism (CE), small artery occlusion (SAA), stroke of other determined etiology (SOE) and stroke of undetermined etiology (SUE) according to TOAST (Trail of ORG 10172 in Acute Stroke Treatment) criteria; and they were divided into mild, moderate and severe types ischemic stroke according to their scores of neurologic impairment.

[Detecting the polymorphism of methylenetetrahydrofolate reductase gene by denaturing high performance liquid chromatography].

Objective: To establish a method for detecting the polymorphism of methylenetetrahydrofolate reductase gene (MTHFR).

Methods: The MTHFR was amplified, and the amplified products were detected by denaturing high performance liquid chromatography (DHPLC), and the amplified MTHFR was confirmed by sequencing and restriction enzyme digesting.

Results: A total of 334 individuals of Han people in southern China were recruited in our study, and their polymorphisms of MTHFR were detected.

[Polymorphisms of ACE-1 and MTHFR genes and genetic susceptibility of ischemic stroke].

Objective: To study the relationships between polymorphisms of angiotensin-converting enzyme 1 (ACE-1) gene and N(5),N(10) -methylene-tetra-hydrofolic acid reductase (MTHFR) gene and ischemic stroke (IS).

Methods: The polymorphisms of ACE-1 and MTHFR genes in the peripheral white blood cells of 143 patients with IS and 154 controls were analyzed using PCR- RFLP.

Results: The frequencies of II, ID, and DD genotypes of ACE-1 gene were 37.