Background/objectives: Mitochondrial genomes are widely used in phylogenetics and evolutionary and ecological research.
Methods: In this study, the newest mitochondrial genome of was assembled and annotated. The comparative mitochondrial genome and selection pressure analyses were used to examine the terrestrial adaptive evolution characteristics of and other terrestrial reptiles.
Genomics Proteomics Bioinformatics
February 2025
Transcriptome-wide association study (TWAS) is a powerful approach for investigating the molecular mechanisms linking genetic loci to complex phenotypes. However, the complexity of the TWAS analytical pipeline including the construction of gene expression reference panels, gene expression prediction, and association analysis using data from genome-wide association studies (GWAS) poses challenges for genetic studies in many species. In this study, we provide the Farm Animal Genotype-Tissue Expression (FarmGTEx) TWAS-server, an interactive and user-friendly multispecies platform designed to streamline the translation of genetic findings across tissues and species.
View Article and Find Full Text PDFDinitroimidazole (DNIm) was recently identified as a powerful bioconjugation agent that could selectively modify thiol over amine on biomolecules at an ultrahigh speed in an aqueous buffer. However, its derivative containing a DNIm module and a terminal alkyne module failed to construct functional agents bearing a DNIm warhead the CuAAC reaction. To solve this problem, a heterobifunctional cross-linker was designed and synthesized by linking a DNIm module with an azide module an oxoaliphatic amido bond spacer arm.
View Article and Find Full Text PDFProtein self-assembly into a crystal in vivo triggers acute or chronic organ injury that can lead to intractable diseases lacking specific treatment options. In this study, we report the discovery of ionic arginine-rich peptides to disrupt the pathogenic galectin-10 (gal-10) crystallization, where the aberrant deposition of gal-10 crystals in airways causes the activation of IL-1β-dependent inflammation and the stimulation of epithelial cells to produce TNF-α. Gal-10 crystals show susceptibility to pH changes and charged residue substitutions at the protein packing interfaces, manifesting the role of charge-charge attractions across protein-protein interaction interfaces in governing gal-10 crystallization.
View Article and Find Full Text PDFBiomineralization of enzymes inside rigid metal-organic frameworks (MOFs) is appealing due to its biocompatibility and simplicity. However, this strategy has hitherto been limited to microporous MOFs, leading to low apparent enzymatic activity. In this study, polysaccharide sodium alginate is introduced during the biomineralization of enzymes in zeolitic imidazolate frameworks (ZIFs) to competitively coordinate with metal ions, which endows the encapsulated enzyme with a 7-fold higher activity than that in microporous ZIFs.
View Article and Find Full Text PDFFor metal-free low-dimensional ferromagnetic materials, a hopeful candidate for next-generation spintronic devices, investigating their magnetic mechanisms and exploring effective ways to regulate their magnetic properties are crucial for advancing their applications. Our work systematically investigated the origin of magnetism of a graphitic carbon nitride (Pca21 CN) monolayer based on the analysis on the partial electronic density of states. The magnetic moment of the Pca21 CN originates from the spin-split of the 2 orbit from special carbon (C) atoms and 2 orbit from N atoms around the Fermi energy, which was caused by the lone pair electrons in nitrogen (N) atoms.
View Article and Find Full Text PDFBackground: Childhood obesity has become a global pandemic, leading to a range of diseases. Childhood obesity appears to be associated with an increased prevalence of sleep apnea syndrome. Sleep apnea is an inestimable risk factor for thrombosis, hypertension, cardiomyopathy and many other diseases.
View Article and Find Full Text PDFObjectives: Porphyromonas gingivalis (P. gingivalis) is a keystone periodontal pathogen associated with various gastro-intestinal tract cancers. However, whether P.
View Article and Find Full Text PDFBackground: African cattle represent a unique resource of genetic diversity in response to adaptation to numerous environmental challenges. Characterising the genetic landscape of indigenous African cattle and identifying genomic regions and genes of functional importance can contribute to targeted breeding and tackle the loss of genetic diversity. However, pinpointing the adaptive variant and determining underlying functional mechanisms of adaptation remains challenging.
View Article and Find Full Text PDFRhodopseudomonas palustris immobilized on multiple materials was used to invistigate Cr(VI) adsorption and bioreduction. The highest Cr(VI) removal (97.5%) was achieved at 276h under the opitimed conditions of 2.
View Article and Find Full Text PDFThe bacteria-algae synergistic wastewater treatment process not only efficiently eliminates nutrients and absorbs heavy metals, but also utilizes photosynthesis to convert light energy into chemical energy, generating valuable bioresource. The study systematically explores the formation, algal species, and regulatory strategies of the bacterial-algal symbiosis system. It provides a detailed analysis of various interaction mechanisms, with a particular focus on nutrient exchange, signal transduction, and gene transfer.
View Article and Find Full Text PDFHeavy metals have been considered an evolving environmental concern due to their harmful and long-lasting impacts. We synthesized a composite of FeS/CS@MIBWS for aqueous Cr(vi) adsorption and reduction utilizing the iron-based waterworks sludge modified by chitosan and FeS. After determining the optimal conditions for the FeS/CS@MIBWS preparation, its Cr(vi) removal capability was evaluated using material characterisation and static Cr(vi) adsorption assays.
View Article and Find Full Text PDFDysregulated Epiregulin (EREG) can activate epidermal growth factor receptor (EGFR) and promote tumor progression in head and neck squamous cell carcinoma (HNSCC). However, the mechanisms underlying EREG dysregulation remain largely unknown. Here, we showed that dysregulated EREG was highly associated with enhanced PDL1 in HNSCC tissues.
View Article and Find Full Text PDFUtilizing hydrogen as a viable substitute for fossil fuels requires the exploration of hydrogen storage materials with high capacity, high quality, and effective reversibility at room temperature. In this study, the stability and capacity for hydrogen storage in the Sc-modified CN nanotube are thoroughly examined through the application of density functional theory (DFT). Our finding indicates that a strong coupling between the Sc-3d orbitals and N-2p orbitals stabilizes the Sc-modified CN nanotube at a high temperature (500 K), and the high migration barrier (5.
View Article and Find Full Text PDFThe production of short-chain fatty acids (SCFAs) is constrained by substrate availability and the increased fractional pressure of H emitted by acidogenic/fermentative bacteria during anaerobic fermentation of waste activated sludge (WAS). This study introduced a novel approach employing zero-valent iron (ZVI)-activated sulfite pretreatment combined with H-consuming sulfate-reducing bacteria (SRB) mediation to improve SCFAs, especially acetate production from WAS fermentation. Experimental results showed that the combined ZVI-activated sulfite and incomplete-oxidative SRB (io-SRB) process achieved a peak SCFAs production of 868.
View Article and Find Full Text PDFThe production of municipal sludge is steadily increasing in line with the production of sewage. A wealth of organic contaminants, including nutrients and energy, are present in municipal sludge. Anaerobic fermentation can be used to extract useful resources from sludge, producing hydrogen, methane, short-chain fatty acids, and, via further chain elongation, medium-chain fatty acids.
View Article and Find Full Text PDFBackground: Human papillomavirus (HPV) integration into the host genome is an important factor in HPV(+)OPSCC carcinogenesis, in conjunction with HPV oncoproteins E6/E7. However, a well-studied investigation about virus-host interaction still needs to be completed. Our objective is to characterise HPV integration to investigate potential mechanisms of tumourigenesis independent of E6/E7 oncoproteins.
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