A method for preparation and use of concentrated serum: experimental study.

Native animal sera are effectively freeze concentrated by slow cooling to -82°C for 18-24 h at a velocity of 0.1-0.5°C/h.

[Possibilities of magnetic-laser therapy in comprehensive treatment of patients with brain concussion in acute period].

The efficacy of magnetic-laser therapy used according to the method developed by us was studied in patients having the brain concussion (BC) in an acute period. The study was based on the dynamics of values of the evoked vestibular potentials and the disease clinical course. It was shown that following the magnetic-laser therapy in combination with traditional pharmacotherapy in BC acute period, the statistically significant positive changes were registered in the quantitative characteristics of the evoked vestibular brain potentials that correlated with the dynamics of the disease clinical course.

[Hyperbaric oxygenation--a method that improves the quality of life for cancer patients in the process of surgical and combined therapy].

The feasibility and advantages of application of hyperbaric oxygenation (HBO)-assisted polychemotherapy have been demonstrated. A procedure for treatment of lymphorrhea following breast surgery has been developed. The therapeutic effect of HBO procedures in postoperative cases of enteroparesis, wound infection and posthemorrhagic anemia has been demonstrated.

Cell-free translation systems from different eukaryotes differ in their sensitivity to a template sugar-phosphate backbone.

To study the role of a messenger sugar-phosphate backbone in the ribosomal decoding process, poly(U) and poly(dT) template activity in different eukaryotic systems has been compared. 80S ribosomes from Saccharomyces cerevisiae appeared to be able to translate poly(dT) both in the presence and in the absence of elongation factors, contrary to poly(U). However, ribosomes from higher eukaryotes (wheat germ, rabbit liver) are completely inefficient in poly(dT) translation.

[Indicators of general and local immunity in chronic and neoplastic processes in the head and neck areas].

Total and secretory immunity was investigated in healthy people, patients with chronic ENT pathologies, and patients with tumors of the oral cavity, larynx and laryngopharynx. It was found that chronic inflammation was followed by a decline of topical defence and a decrease of total immunity. In cancer patients these changes were aggravated.

[Synthesis of ovalbumin and globin during simultaneous translation of their mRNA in the presence of tRNA of different origin in the cell-free protein-synthesizing system from wheat germ].

Competition among ovalbumin and globin mRNA under their simultaneous translation in the tRNA-dependent cell-free system from wheat germ was studied. One of the mRNAs was added to samples in a constant amount, that provided 50% protein synthesis level of the maximum, other--in increasing amount (from 0 to the maximum). The ration of ovalbumin and globin synthesis rates has been shown to depend essentially (1.

[Modulation of the selective translation of mRNA in cell-free system exposed to tRNA].

The homo- and heterologous tRNA were studied for their influence on the synthesis rate of globin and ovalbumin under simultaneous translation of their mRNAs in the tRNA-dependent cell-free system from wheat germ. In the presence of the fixed concentrations of one of the mRNAs the different amounts of the other mRNA (from 6 up to saturation of the system) were added to the samples. The ratio of the synthesis rate of the translation products after their electrophoretic characterization is determined on the basis of the autoradiogram scanning.

[Effects of hyperbaric oxygenation on tumor growth].

The study failed to show a stimulating effect of hyperbaric oxygen on growth and metastatic spreading of sarcoma 37 transplanted subcutaneously or intravenously to female SHR mice, melanoma B16 intramuscularly transplanted to female C57/Bl mice and methylcholanthrene-induced tumors in female albino noninbred rats. An insignificant inhibitory effect of hyperbaric oxygen on transplantable tumor growth and development of inducible ones in experimental animals was observed.

[Functional state of the adrenals in lung cancer].

Catecholamines and cortisol in blood were studied in 91 lung cancer patients and in 11 patients with benign neoplasms in the lung. The investigation peformed has shown a certain dependence between the process stage and the sympatho-adrenal system functioning. Total amount of catecholamines is increased as the disease progresses, reaching the maximum at stage III and is sharply decreased at stage IV.

[Comparative evaluation of the adrenal functional state in the surgical and combined treatment of lung cancer].

The functional state of the adrenal glands diring surgical and combination treatment was examined in 38 radically operated patients with pulmonary cancer. Irradiation of lung cancer patients was found to stimulate the adrenal glands activity followed by reduction of their potentalities, manifested in a less marked increase of the catecholamines level and decreased 11-OCS level in blood during surgical treatment.