Salicylic Acid-Induced Expression Profiles of LRR and LRR-RLK Candidate Genes Modulate Resistance in Blackgram and Its Two Wild Non-Progenitors.

Blackgram is an important short-duration grain legume, but its yield is highly affected by various stresses. Among biotic stresses, yellow mosaic disease (YMD) is known as a devastating disease that leads to 100% yield loss under severe conditions. The cultivated lines possess resistance, but exploring more diverse sources of resistance may be useful for pyramiding to improve the durability of said resistance.

Locked Dimerized CXCL12 Exerts Radiosensitizing Effects in Head and Neck Cancer.

Background: Head and neck squamous cell carcinoma (HNSCC) presents significant treatment challenges, particularly in cases unrelated to human papillomavirus (HPV). The chemokine receptor CXCR4, interacting with its ligand CXCL12, plays a crucial role in tumor proliferation, metastasis, and treatment resistance. This study explores the therapeutic potential of engineered monomeric and dimerized CXCL12 variants (CXCL12 and CXCL12, respectively) in HNSCC and evaluates potential additive effects when combined with radiation therapy.

Cooperative Molecular Interaction-Based Highly Efficient Capturing of Ultrashort- and Short-Chain Emerging Per- and Polyfluoroalkyl Substances Using Multifunctional Nanoadsorbents.

The short-chain (C to C) and ultrashort-chain (C to C) per- and polyfluoroalkyl substances (PFAS) are bioaccumulative, carcinogenic to humans, and harder to remove using current technologies, which are often detected in drinking and environmental water samples. Herein, we report the development of nonafluorobutanesulfonyl (NFBS) and polyethylene-imine (PEI)-conjugated FeO magnetic nanoparticle-based magnetic nanoadsorbents and demonstrated that the novel adsorbent has the capability for highly efficient removal of six different short- and ultrashort-chain PFAS from drinking and environmental water samples. Reported experimental data indicates that by capitalizing the cooperative hydrophobic, fluorophilic, and electrostatic interaction processes, NFBS-PEI-conjugated magnetic nanoadsorbents can remove ∼100% short-chain perfluorobutanesulfonic acid within 30 min from the water sample with a maximum absorption capacity of ∼234 mg g.

Okanin alleviates symptoms of nociceptive-like responses in diabetic peripheral neuropathy in type 1 diabetic Wistar rats by regulating the AGEs/NF-κB/Nrf-2 pathway.

Elevated reactive species and AGEs contribute to deregulation of transcription factors e.g., NF-κB and Nrf2 in diabetic peripheral neuropathy (DPN).

Genetic association between Class II division 1 and division 2 malocclusions with PAX9 (rs8004560) gene polymorphism in a tertiary care hospital.

Objective: To assess whether there is any difference in the genetic association between Class II division 1 (div. 1) and division 2 (div. 2) malocclusions using PAX9 (rs8004560) gene single nucleotide polymorphism (SNP).

Effect of soluble fiber supplementation on lipid parameters in subjects with type 2 diabetes: A systematic review and meta-analysis of randomized controlled trials.

Background: There is no consensus in the existing literature regarding the effect of soluble fiber on the lipid profile of patients with type 2 diabetes mellitus (T2DM). This systematic review and meta-analysis of randomized controlled trials aimed to assess the effect of soluble fiber on triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in T2DM patients.

Methods: PubMed/MEDLINE, Scopus, and ISI Web of sciences were searched for RCTs up to 4 May 2024.

Tunneled dialysis catheter: Simple, re-do, complicated.

Tunneled dialysis catheter (TDC) placement plays an essential role in hemodialysis patients. These catheters are inserted in a significant number of patients prior to establishing permanent dialysis access. Insertion is often simple and straightforward, and the preference is to use the right internal jugular vein due to fewer chances of long-term complications.

A Comprehensive Safety Assessment of Algae Protein from Picochlorum for Human Consumption.

The current trend happens to be that consumers are seeking nourishing, high quality sustainable protein sources to meet their nutritional needs, thus establishing a clear intent to broaden their protein horizon. Microalgae protein holds great promise in becoming the next vegan protein option. In the present study, protein extracted from the microalga Picochlorum maculatum has been thoroughly evaluated for its safety for human consumption through a battery of in-vivo and in-vitro tests.

BRAF V600E mutation and high expression of PD-L1 in Rosai-Dorfman disease: case report and review of the literature.

BRAF V600E mutations are frequently found in histiocytic/dendritic cell neoplasms such as Erdheim-Chester disease (ECD) and Langerhans cell histiocytosis (LCH), but few reports have also described BRAF mutations in Rosai-Dorfman disease (RDD), and even these cases may predominantly represent mixed histiocytosis. BRAF mutations have been studied in histiocytic/dendritic cell neoplasms and described to be associated with increased risk of relapse and long-term consequences, but few studies have examined BRAF V600E mutation in RDD, which is recognized as a neoplasm given the high frequency of MAPK pathway alterations. Here, we report a case of BRAF V600E-mutated RDD in a patient who presented with generalized lymphadenopathy.

Unlocking gut-liver-brain axis communication metabolites: energy metabolism, immunity and barriers.

The interaction between the gut-microbiota-derived metabolites and brain has long been recognized in both health and disease. The liver, as the primary metabolic organ for nutrients in animals or humans, plays an indispensable role in signal transduction. Therefore, in recent years, Researcher have proposed the Gut-Liver-Brain Axis (GLBA) as a supplement to the Gut-Brain Axis.

Genome-Wide Profiling of Histone Modifications in Fission Yeast Using CUT&Tag.

Eukaryotic DNA is organized in the nucleus in the form of chromatin. Nucleosomes, the fundamental unit of chromatin, are subject to many posttranslational modifications (PTMs) as well as compositional variations through incorporation of histone variants. These alterations play important roles in regulation of genome structure and activity.

ChIPmentation for Epigenomic Analysis in Fission Yeast.

Histone modifications and transcription factor-DNA interactions regulate vital processes such as transcription, recombination, repair, and accurate chromosome segregation. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) has been instrumental in studying genome-wide distribution of DNA-bound or chromatin-associated factors and histone posttranslational modifications (PTMs). Here, we describe a ChIPmentation protocol adapted for fission yeast, Schizosaccharomyces pombe.

One-Pot Green Synthesis and Biological Evaluation of Dimedone-Coupled 2,3-Dihydrofuran Derivatives to Divulge Their Inhibition Potential against Staphylococcal Thioredoxin Reductase Enzyme.

New therapeutic leads are in global demand against multiple drug-resistant , as presently there is no drug of choice left to treat this pathogen. In the present work, we have designed, synthesized, and in vitro validated dimedone-coupled 2,3-dihydrofuran (DDHF)-based inhibitor scaffolds against Staphylococcal thioredoxin reductase (SaTR), a pivotal drug target enzyme of Gram-positive pathogens. Accordingly, a green multicomponent method that is both efficient and one pot has been optimized to synthesize DDHF derivatives.

Divergent modulation of activated protein C pleiotropic functions by antibodies that differ by a single amino acid.

Activated protein C (APC) is a pleiotropic plasma protease with diverse functions derived from its anticoagulant, anti-inflammatory, and cytoprotective activities. The selective uncoupling and/or modulation of these APC activities by antibodies may have therapeutic benefit in diseases such as traumatic bleeding, hemophilia, sepsis, and ischemia. TPP-26870 is an antibody that targets a nonactive site of APC for the selective modulation of APC activities.

Bacteriological profile and antibiotic sensitivity pattern of bloodstream infections from a teaching institution in Eastern Uttar Pradesh, North India.

Introduction: Bloodstream infections (BSIs), encompassing both self-limiting bacteremia and potentially fatal septicaemia, make up the majority of healthcare-associated ailments worldwide. The organisms encountered are mostly multidrug-resistant (MDROs), leading to increased hospital stays. Our study aims to collect data about blood culture isolates from a medical college in eastern Uttar Pradesh, India.

Differential cellular communication in tumor immune microenvironment during early and advanced stages of lung adenocarcinoma.

Heterogeneous behavior of each cell type and their cross-talks in tumor immune microenvironment (TIME) refers to tumor immunological heterogeneity that emerges during tumor progression and represents formidable challenges for effective anti-tumor immune response and promotes drug resistance. To comprehensively elucidate the heterogeneous behavior of individual cell types and their interactions across different stages of tumor development at system level, a computational framework was devised that integrates cell specific data from single-cell RNASeq into networks illustrating interactions among signaling and metabolic response genes within and between cells in TIME. This study identified stage specific novel markers which remodel the cross-talks, thereby facilitating immune stimulation.

Proteostasis and Its Role in Disease Development.

Proteostasis (protein homeostasis) refers to the general biological process that maintains the proper balance between the synthesis of proteins, their folding, trafficking, and degradation. It ensures proteins are functional, locally distributed, and appropriately folded inside cells. Genetic information enclosed in mRNA is translated into proteins.

Psychometric Properties of the Brief COPE Among Sexual Minority Adults.

Background: The Brief COPE is a widely used coping instrument, but there is a paucity of research regarding its ability to measure coping among sexual minority (SM) persons.

Objective: This study determined the psychometric properties of the Brief COPE and identified coping domains among a sample of SM individuals.

Methods: An online survey was conducted with 530 SM persons in the United States.

Melatonin Augments the Expression of Core Transcription Factors in Aged and Alzheimer's Patient Skin Fibroblasts.

Alzheimer's disease (AD) is a progressive neurodegenerative disorder. Altered neurogenesis and the appearance of AD pathological hallmarks are fundamental to this disease. SRY-Box transcription factor 2 (Sox2), octamer-binding transcription factor 4 (Oct4), and Nanog are a set of core transcription factors that play a very decisive role in the preservation of pluripotency and the self-renewal capacity of embryonic and adult stem cells.