[M-29 human diploid cells line--a substrate for the production of antiviral vaccines].

Aim: Study of morphologic and karyologic characteristics of 5 russian human diploid cell lines (HDC).

Materials And Methods: 5 HDC lines and HDC strain MRC-5 were studied; RK-13 and Vero continuous cell lines were used; viruses: rubella (RA27/3), measles (L-16), epidemic parotitis (L-3). Cytogenetic analysis of HDC was performed by using DAPI differential staining method.

[The antiviral action of recombinant forms of the human T-lymphocyte CD4 receptor].

A recombinant protein containing the first 179 N-terminus amino acids of human T-lymphocyte CD4-receptor was synthesized in E. coli cells. This recombinant protein was shown to interact with OKT4A and Leu3a monoclonal antibodies competing with HIV gp120 glycoprotein for binding with the native CD4 receptor.

[The isolation and characteristics of monoclonal antibodies to recombinant proteins of HIV-1 and HIV-2--the env and gag gene products].

Ten hybridomas secreting monoclonal antibodies (Mab) against recombinant HIV-1 and HIV-2 antigens were produced (3 Mab anti-gag protein, 2 anti-env1, and 5 anti-env2). In the immunoblotting assay all the anti-gag Mabs reacted with HIV capsid protein p24, whereas one of them reacted also with p55 protein and with 7 other polypeptides. Another anti-gag Mab cross-reacted with the antigen of subpopulation of human peripheral blood lymphocytes.

[Characteristics of Namalwa cells--a substrate for production of human interferon].

The properties of lymphoid Namalwa cell line propagated at the USSR Academy of Medical Sciences Research Institute of Viral Preparations for interferon production are described. The scanning and transmissive electron microscopy studies of the cells showed their morphological stability and the absence of microbial contamination. The 46-48-chromosome cells comprised 85% of the population, hypodiploid cells (44-45 chromosomes), 9%, tetraploid and hypertetraploid cells, 3%.

[Isolation of purified and concentrated human interferon from Namalwa cells].

The interferon produced in the cultured Namalwa cells was purified and concentrated according to the method of K. Cantell and S. Hirvonen developed for purification of leukocyte interferon.

[ELISA immunoenzyme detection of the antigens of interferon and protein contaminants].

The possibility of specific detection of interferon antigens by ELISA was demonstrated on a model of partially purified and ultrafiltration-concentrated preparations of mouse fibroblast interferon. ELISA allows differentiation of specific interferon antigens and the main protein contaminants of its preparations: bovine serum albumin and virus-inducer proteins.

[Study of LPV oncornavirus reproduction in mixed cell culture].

Electron microscopy and mathematical analysis were used to determine the intensity of LPV oncornavirus production by a single cell in co-cultivated human diploid cells and cells of the continuous T-9 line. Maximum production of intracytoplasmic particles of A type was observed at 48 hours of cultivation, and extracellular virions at 96 hours. Mature virions of B type were more numerous than mature virions of C type in the mixed culture.

[Clone cells obtained from continuous cultures and differing in their sensitivity to tick-borne encephalitis viruses].

Studies on cloning of continuous HEp-2 and SPEV cell lines were carried out. The sensitivity of the resulting clones to tick-borne encephalitis virus was determined and the clone lines were shown to be heterologous in their sensitivity to TBE virus by means of the immunofluorescence and virological methods. Among 47 clones of HEp-2 line virus reproduction was observed in 30 clones, no reproduction was demonstrated in 17 clones.