Publications by authors named "Shujing Qiao"
CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of , with the highest efficiency of 85%.
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- The study examined how adding nitrogen at different times affects wine ester production during fermentation with two types of yeast, Hanseniaspora uvarum and Saccharomyces cerevisiae.
- Adding 50-200 mg/L of yeast assimilable nitrogen (YAN) at the start of fermentation had a minor impact on yeast competition and ester profiles.
- However, supplementing nitrogen mid-fermentation greatly improved the survival of Hanseniaspora uvarum and led to increased levels of certain esters, with changes in carbon fluxes contributing to this enhanced production.