DYRK1A-TGF-β signaling axis determines sensitivity to OXPHOS inhibition in hepatocellular carcinoma.

Intervening in mitochondrial oxidative phosphorylation (OXPHOS) has emerged as a potential therapeutic strategy for certain types of cancers. Employing kinome-based CRISPR screen, we find that knockout of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) synergizes with OXPHOS inhibitor IACS-010759 in liver cancer cells. Targeting DYRK1A combined with OXPHOS inhibitors activates TGF-β signaling, which is crucial for OXPHOS-inhibition-triggered cell death.

Rapid and accurate determination of diesel multiple properties through NIR data analysis assisted by machine learning.

The rapid and accurate detection of diesel multiple properties is an important research topic in petrochemical industry that is conducive to diesel quality assessment and environmental pollution mitigation. To that end, this paper developed a new machine learning model for near infrared (NIR) spectroscopy capable of simultaneously determining diesel density, viscosity, freezing point, boiling point, cetane number and total aromatics. The model combined improved XY co-occurrence distance (ISPXY) and differential evolution-gray wolf optimization support vector machine (DEGWO-SVM) to attain the goal of rapidity and accuracy.

Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene.

Bone marrow-derived mesenchymal stem cells can differentiate into a variety of adult cells. Brain-derived neurotrophic factor (BDNF) is briefly active during differentiation and induces mesenchymal stem cells to differentiate into nerve cells. In this study, we cloned human BDNF to generate a recombinant pcDNA3.