[Scientificity of traditional commodity grade of Chinese medicinal materials--taking Lonicerae Japonicae Flos as example].

Taking Lonicerae Japonicae Flos as an example, the method of "expert consensus of different regions" was used to screen the representative samples and evaluate their commodity grades. The correlation analysis, hierarchical cluster analysis and partial least squares discriminant analysis(PLS-DA) of "commodity grade-appearance characteristic-component content" were carried out to reveal the scientificity of traditional commodity grade of Chinese medicinal material. By referring to the existing literature and the grade investigation from the sample collection regions, 78 "initial grade" samples were screened out from 118 collected samples.

[Research on HPLC fingerprint of Rheum palmatum from Gansu Province called "quan-shui-da-huang"].

Objective: To establish an analysis method for HPLC fingerprint of Rheum palmatum collected from Gansu province (called "Quan-shui-da-huang"), and provide basis for quality control of Quan-shui-da-huang.

Methods: The Cosmosil 5C18-PAQ column (250 mm x 4.6 mm, 5 microm) was used with the mixture of 0.

Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract.

Objective: To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography (HPLC) and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae.

Methods: The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J'sphere ODS-H80 column (4.6 mm×250 mm, 5 μm) with acetonitrile-water-tetrahydrofuran-acetic acid (41:59:1:0.

[Preparation of licorice juice as a processing excipient].

Objective: To develop the preparation method of licorice juice as a processing excipient and to determine the proper proportion of licorice juice and fructus evodiae during process.

Method: Nine experiments were carried out through L9 (3(4)) orthogonal design. The wetting results were compared by using different proportion of licorice juice and fructus evodiae.

[The HPLC fingerprint analysis of Fructus citri].

Objective: To study and establish the fingerprint of raw Fructus Citri by phased high performance liquid chromatography.

Method: The chromatographic conditions were as follows: a Kromasil C18 column was used; the mobile phase was composed of methanol and acetic-water(3.1:100, v/v) with gradient elution; the flow rate was 1.

[Effects of different types and standard of processing vinegaron inherent constituents in rhizoma of Corydalis yanhusuo].

Objective: To investigate the effects of processing adjuvants-different types of processing vinegar on effective constituents in rhizoma of Corydalis yanhusuo, and evaluate the quality of different types of vinegar in China.

Method: The HPLC method was adopted to determine the extraction solubility of dl-tetrahydropalmation and total alkaloids in rhizoma of Corydalis yanhusuo processed by vinegar. The sample extracts were separated on kromasil ODS column with mobile phase of methanol-1% phosphoric acid solution(65:35) and detection wavelength was 280 nm.

[Evaluation of green tangerine peel processed with vinegar using HPLC fingerprint technique].

Objective: To develop an HPLC method for determination of fingerprints and evaluate green tangerine peel (GTP) processed with vinegar.

Method: The samples were extracted with 50% ethanol using ultrasonic bath and analyzed on a Chromasil C18 column eluted with methanol and water containing 0.2% acetic acid in a linear gradient program.

[Determination of synephrine and N-methyltyramine in Pericarpium Citri Reticulatae viride by HPLC].

Objective: To establish a quantitative method for determination of synephrine and N-methyltyramine in Citri Reticulatae.

Method: Samples were extracted with 30% methanol. ODS column was used with methanol-water-sodium dodecyl sulfate (55:45:0.