3-Fucosyllactose (3-FL), an essential component of human milk oligosaccharides, is crucial for infant health. However, the extraction of 3-FL from milk presents a significant challenge. In this study, BL21 Star (DE3) was engineered for 3-FL biosynthesis, and the gene combinations and metabolic pathways were optimized to obtain a high-yield 3-FL production strain.
View Article and Find Full Text PDFAlthough some progress has been made in the treatment of cancer, challenges remain. In recent years, advancements in nanotechnology and stem cell therapy have provided new approaches for use in regenerative medicine and cancer treatment. Among them, magnetic nanomaterials have attracted widespread attention in the field of regenerative medicine and cancer; this is because they have high levels of safety and low levels of invasibility, promote stem cell differentiation, and affect biological nerve signals.
View Article and Find Full Text PDFAntiangiogenesis therapies targeting vascular endothelial growth factor (VEGF) have revolutionized the treatment of neovascular ocular diseases, including neovascular age-related macular degeneration (nAMD). Compelling evidence has implicated the vital role of complement system dysregulation in AMD pathogenesis, implying it as a potential therapeutic strategy for geographic atrophy in dry AMD and to enhance the efficacy of anti-VEGF monotherapies in nAMD. This study reports the preclinical assessment and phase 1 clinical outcomes of a bispecific fusion protein, efdamrofusp alfa (code: IBI302), which is capable of neutralizing both VEGF isoforms and C3b/C4b.
View Article and Find Full Text PDFFront Public Health
April 2022
Background: The commonly used NEMA IEC Body phantom has a number of defects, hindering its application for detecting micro-lesions and measuring the performance parameters of computed tomography (CT). This study aimed to propose a PET/CT phantom designed by National Institute of Metrology (NIM), China, which is capable of simultaneously testing the performance of PET and CT systems, and to evaluate the quality of imaging.
Methods: The phantom developed in the present study, the NIM PET/CT phantom, is composed of a PET imaging module and a CT imaging module, and these modules are connected together through bolts, which can simultaneously measure the imaging performance of PET and CT systems.
Background: China approved adalimumab for the treatment of ankylosing spondylitis in 2013. However, the cost of the standard dose regimen exceeds ¥15 000 (around US$2250) per month, which is well beyond affordability for most Chinese patients. No biosimilars of adalimumab are available in China; IBI303 is a monoclonal antibody against TNFα that is currently in development.
View Article and Find Full Text PDFObjective: To study the direct effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury and elucidate their therapeutic mechanism on ischemic heart diseases.
Method: Cultured cardiomyocytes of neonatal rats were subjected to anoxia-reoxia injury which simulated the ischemia-reperfusion injury in vivo, and hawthorn leaf procyanidins were applied. The therapeutic effect was valued by LDH leakage and MTT test.