Publications by authors named "Shujaat A Khan"

Purpose: Biallelic INPP4A variants have recently been associated with severe neurodevelopmental disease in single-case reports. Here, we expand and elucidate the clinical-genetic spectrum and provide a pathomechanistic explanation for genotype-phenotype correlations.

Methods: Clinical and genomic investigations of 30 individuals were undertaken alongside molecular and in silico modelling and translation reinitiation studies.

View Article and Find Full Text PDF
Article Synopsis
  • * Researchers used whole exome sequencing to study nine Pakistani families with OCA, identifying pathogenic gene variants through advanced sequencing techniques and analysis tools.
  • * The study discovered specific gene variants in TYR, OCA2, and HPS1, including two new mutations, enhancing understanding of OCA's genetic basis and aiding in better management for affected families in Pakistan.
View Article and Find Full Text PDF
Article Synopsis
  • - A series of novel norfloxacin analogs were synthesized and characterized using NMR and mass spectrometry to assess their biological activity.
  • - One specific compound showed impressive potency against HeLa cancer cells, achieving 100% inhibition of cell viability at an IC of 3.1 ± 0.2 μM, while all compounds displayed moderate to excellent antioxidant properties.
  • - The compounds adhered to Lipinski's rule of drug-likeness and demonstrated favorable absorption characteristics, indicating potential for future development as anticancer drugs.
View Article and Find Full Text PDF
Article Synopsis
  • The research focused on creating new chloroquine analogues to test their effectiveness against microbes and cancer cells.
  • The synthesized analogues were found to have significant antimicrobial properties, with one analogue showing a 30.3 mm zone of inhibition against a specific microbe.
  • Additionally, one analogue demonstrated strong cytotoxic effects on HeLa cells, achieving 100% cell inhibition at a low concentration, outperforming the original chloroquine.
View Article and Find Full Text PDF

Glipizide, a poor water-soluble drug belongs to BCS class II. The proposed work aimed to enhance the solubility of glipizide by preparing solid dispersions, using polyvinyl pyrrolidone (PVP) and polyethylene glycol (PEG). Solvent evaporation method was used for the preparation of glipizide solid dispersions.

View Article and Find Full Text PDF

Currently, hydrogels are considered as ideal biomaterials due to their unique structure and characteristics that facilitates considerable hydrophilicity, swelling, drug loading and release. In this study, we report pH-responsive GG-MAA-AMPS hydrogel delivery system prepared via free radical polymerization technique. Hydrogels were loaded with Metformin HCl as a model drug.

View Article and Find Full Text PDF

The biopolymers-based two-fold system could provide a sustained release platform for drug delivery to the brain resisting the mucociliary clearance, enzymatic degradation, bypassing the first-pass hepatic metabolism, and BBB thus providing superior bioavailability through intranasal administration. In this study, poloxamers PF-127/PF-68 grafted chitosan HCl-co-guar gum-based thermoresponsive hydrogel loaded with eletriptan hydrobromide laden pullulan nanoparticles was synthesized and subjected to dynamic light scattering, Fourier transform infrared spectroscopy, thermal analysis, x-ray diffraction, scanning electron microscopy, stability studies, mucoadhesive strength and time, gel strength, cloud point assessment, rheological assessment, ex-vivo permeation, cell viability assay, histology studies, and in-vivo Pharmacokinetics studies, etc. It is quite evident that CSG-EH-NPs T-Hgel has an enhanced sustained release drug profile where approximately 86 % and 84 % of drug released in phosphate buffer saline and simulated nasal fluid respectively throughout 48 h compared to EH-NPs where 99.

View Article and Find Full Text PDF

Background: Spontaneous Adverse drug reactions (ADRs) reporting is a cornerstone for a successful pharmacovigilance program as under-reporting of ADRs remains a major issue around the globe. The current study aimed to assess the knowledge attitude and practices of health care professionals regarding pharmacovigilance along with barriers and factors to encourage ADR reporting at tertiary care hospitals of Khyber-Pakhtunkhwa, Pakistan.

Methods: A questionnaire-based cross-sectional survey was conducted, using the convenience sampling method to collect the data from doctors, nurses, and pharmacists working in seven tertiary care hospitals from seven districts of Khyber-Pakhtunkhwa province, Pakistan, between July 2019 and March 2020.

View Article and Find Full Text PDF

Drug safety assures the effectiveness, safety, and security of drugs, vaccines, and other biologicals to protect public health. Medication-related errors coupled with unjudicial medication practices often cause a catastrophic impact on the healthcare system globally. The present study aimed to assess the knowledge, attitude, and practice of physicians toward pharmacovigilance and barriers to adverse drug reaction (ADR) reporting at tertiary care hospitals in Khyber-Pakhtunkhwa (KP) province, Pakistan.

View Article and Find Full Text PDF

Injectable hydrogels with multifunctional tunable properties comprising biocompatibility, anti-oxidative, anti-bacterial, and/or anti-infection are highly preferred to efficiently promote diabetic wound repair and its development remains a challenge. In this study, we report chondroitin sulphate (CS) and sodium alginate (SA)-based injectable hydrogel using solvent casting method loaded with curcumin that could potentiate reepithelization, increase angiogenesis, and collagen deposition at wound microenvironment to endorse healing cascade. The physical interaction and self-assembly of chondroitin sulfate grafted alginate (CS-Alg-g-PF127) hydrogel were confirmed using nuclear magnetic resonance (H NMR) and Fourier transformed infrared spectroscopy (FT-IR), and cytocompatibility was confirmed by fibroblast viability assay.

View Article and Find Full Text PDF

Conventional formulations of antiviral drug acyclovir have various limitations such as low bioavailability. The current study was aimed at developing polymeric matrices for the controlled delivery of acyclovir using sericin as polymer and acrylic acid (AA) as a monomer. The free radical polymerization technique was used for hydrogel formulation.

View Article and Find Full Text PDF

The current study is an attempt to explore the effect of varying quantities of hydroxypropyl cellulose (HPC) polymer on carbamazepine (CBZ) cocrystal formation with dicarboxylic acid coformers i.e., malonic acid (MA), succinic acid (SA), glutaric acid (GA), and adipic acid (AA).

View Article and Find Full Text PDF

Background: Oxaliplatin (OXP), a 3rd generation platinum compound, which causes severe side effects due to; impulse high concentration in the bloodstream thereby exposing healthy cells at a high ratio, nonspecific delivery at the target site and non-compliance is administered intravenously.

Objective: The project was aimed at the development, characterization, and in-vitro and in-vivo evaluation of pHresponsive hydrogels for oral administration of OXP.

Methods: Hydrogel formulations were synthesized through a free radical polymerization technique followed by brief characterization using various techniques.

View Article and Find Full Text PDF
Article Synopsis
  • Alzheimer's disease is primarily marked by memory issues, behavioral changes, and cognitive decline, with aging being the biggest risk factor.
  • Research has shown that traditional plants from Asia and Europe can have a positive impact on aging and neurodegenerative pathways related to Alzheimer's.
  • There's a growing interest in ethnobotanicals for improving memory and cognition, as many people worldwide still rely on natural remedies, prompting a shift in the pharmaceutical industry towards herbal formulations for drug discovery.
View Article and Find Full Text PDF

Objectives: Thyroid Cancer is one of the rarest cancers but its prevalence has been increasing worldwide for the last couple of decades.

Methods: The data collection tool was designed to assess knowledge, awareness, perception, and attitude towards preventive practices of thyroid cancer in Pakistani university students. The data were collected over a duration of six months and a total number of 3722 students participated.

View Article and Find Full Text PDF

Background: Staphylococcus aureus (S. aureus) is the most common infectious agent in the community and hospitals. Infections with S.

View Article and Find Full Text PDF

Medicinal plants of Pakistan are known for their curative properties against snake bite as rural people have been using natural herbs for such injuries for hundreds to thousands of years. People of rural areas of Pakistan are prone to snakebite, and on the whole death due to snakebite has been increasing worldwide. The objective of this study was to test the neutralizing potential of 17 Pakistani medicinal plant extracts against phospholipase A2 activity in Echis carinatus venom.

View Article and Find Full Text PDF

Objective: The aim of the present study was to design an efficient delivery system with an anticipated swelling and drug release properties for a prolonged drug release as well as to target colon for various hydrophilic drugs.

Method: For this purpose, the pH-responsive hydrogel comprising a combination of Eudragit and acrylic acid was formed. The hydrogels were characterized for spectral (FTIR), thermal (TGA/DSC), structural (XRD), and morphological (SEM) investigations.

View Article and Find Full Text PDF

is a multifunctional plant that has gained acceptance as an excellent home remedy source in Asia and the world. The present study was intended to evaluate the phytochemical contents and antioxidant, antimicrobial, antileishmanial, and protein kinase inhibition activities in different fractions of leaf. Methanolic extract of leaves was fractionated using column chromatography and ten fractions (AV1-AV10) were obtained.

View Article and Find Full Text PDF

This study describes the fabrication of chemically crosslinked pectin-based LA-co-MAA hydrogels through free radical polymerization technique for the colonic delivery of oxaliplatin. Methylene bisacrylamide was used as a crosslinking agent and ammonium persulfate as an initiator. The successful fabrication and drug loading were confirmed through Fourier transform infrared spectroscopy (FTIR).

View Article and Find Full Text PDF

To investigate the effects of zinc oxide nanoparticles (ZnO NPs) on chloroquine (CQ)-induced itching, and overall behavior of mice after oral administration of ZnO NPs of various sizes and doses. With the wide-spread use of ZnO NPs in pharmaceuticals and cosmetics, concerns about their safety and toxicity are also increasing. Multiple aspects of ZnO NPs regarding cytotoxicity and tolerability are under investigation globally.

View Article and Find Full Text PDF

In this study, the chemically crosslinked hydrogels of carboxymethyl chitosan and polyvinyl alcohol were fabricated by the free radical polymerization. Successful polymerization was confirmed by Fourier transform infrared spectroscopy, while x-ray diffraction, thermogravimetric analysis, and differential scanning calorimetric measurements helped in investigating crystallinity and thermal behavior of the products. Morphological characterization was performed by scanning electron microscopy.

View Article and Find Full Text PDF

Objective: The study describes the development of chitosan-based (AMPS-co-AA) semi-IPN hydrogels using free radical polymerization technique.

Methods: The resulting hydrogels were characterized using Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), X-Ray diffraction (XRD), and Scanning Electron Microscopy (SEM). The successful crosslinking of chitosan, 2- Acrylamido-2-Methylpropane Sulfonic Acid (AMPS), and Acrylic Acid (AA) was confirmed by FT IR.

View Article and Find Full Text PDF

The aim of this study was to prepare hydroxypropyl-methyl cellulose (HPMC)-K15 based hydrogels via free radical polymerization using 2‑acrylamido-2‑methyl propane sulphonic acid (AMPS) as a monomer and N,N'-methylene bisacrylamide (MBAAm) as a crosslinker. Loxoprofen sodium was chosen as a model drug and successfully loaded in hydrogel discs. Sol-gel, porosity, loading efficiency, and the in vitro drug release analysis were carried out to evaluate its drug delivery potential.

View Article and Find Full Text PDF