The biological characteristics of DAstV molecular epidemiology and pathogenicity of duck astrovirus causing hepatitis in ducks and chickens in Southeast China.

A suspected outbreak of duck astrovirus (DAstV) disease occurred in a large Muscovy duck farm in Guangdong Province, China, in June 2022, which severely affected the production performance and health of Muscovy ducks. This study aimed to investigate the prevalence of DAstV disease in Southeast China. Herein, we employed semi-nested PCR ethodto screen 5203 swab and liver samples from 11 Muscovy duck farms in 5 provinces of China for the presence of DAstV.

Effects of a complex probiotic preparation, Fengqiang Shengtai and coccidiosis vaccine on the performance and intestinal microbiota of broilers challenged with Eimeria spp.

Background: Coccidiosis, a prominent intestinal protozoan disease, carries significant economic implications for the poultry industry. The aim of this study was to evaluate the effects of Fengqiang Shengtai (BLES), a probiotics product, and coccidiosis vaccine in modulating the intestinal microbiome and providing insight into mitigating the occurrence and management of avian coccidiosis.

Methods: Broilers included in the study were divided into four pre-treatment groups: the Pre-Con group (commercial diet), Pre-BLES group (BLES supplement), Pre-Vac group (coccidiosis vaccination) and Pre-Vac-BLES group (combined vaccination and BLES).

Molecular characterization of chicken infectious anaemia virus (CIAV) in China during 2020-2021.

Chicken infectious anaemia virus (CIAV) has been identified as the causative agent of chicken infectious anaemia (CIA), causing huge economic losses to the poultry industry globally. In this study, a total of 573 clinical samples were collected from 197 broiler farms in 17 provinces of China during 2020-2021. Among them, 375 samples (375/573, 65.

Effects of Bacillus subtilis and coccidiosis vaccine on growth indices and intestinal microbiota of broilers.

Avian coccidiosis is the most serious parasitic disease in the poultry industry. Therefore, the aim of the current study was to explore the effects of B. subtilis and live coccidiosis vaccine alone or in combination on the production performance and anticoccidiosis, as well as the dynamic changes of intestinal microbial community.

Molecular characterization and analysis of the ATPase ASNA1 homolog gene of Eimeria tenella in a drug sensitive strain and drug resistant strains.

The widespread use of drugs has exacerbated the resistance of Eimeria tenalla to anti-coccidial drugs. Using RNA-seq, we previously found the ATPase ASNA1 homolog of E. tenella (EtASNA1) was differentially expressed in resistant strains and drug sensitive (DS) strain.

Translation Initiation Factor eIF-5A That Interacts With Calcium-Dependent Protein Kinase 4 Is Involved in Host Cell Invasion.

is an apicomplexan, parasitic protozoan known to infect poultry worldwide. An important calcium-dependent protein kinase (CDPK) has been identified in plants, green algae, ciliates and apicomplexan, such as . CDPKs are effector molecules involved in calcium signaling pathways, which control important physiological processes such as gliding motility, reproduction, and host cell invasion.

Molecular characterization and functional analysis of Eimeria tenella citrate synthase.

Chicken coccidiosis, caused by an obligate intracellular protozoan parasite of the genus Eimeria, is a major parasitic disease in the intensively reared poultry industry. Due to the widespread use of anticoccidial drugs, resistance has become an inevitable problem. In our previous study, Eimeria tenella citrate synthase (EtCS) was found to be up-expressed in two drug-resistant strains (diclazuril-resistant and maduramycin-resistant strains) compared to drug-sensitive strain by RNA sequence.

Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1.

Apical membrane antigen 1 (AMA1) is a type I integral membrane protein that is highly conserved in apicomplexan parasites. Previous studies have shown that Eimeria tenella AMA1 (EtAMA1) is critical for sporozoite invasion of host cells. Here, we show that EtAMA1 is a microneme protein secreted by sporozoites, confirming previous results.

Identification and Characterization of a Novel Apical Membrane Antigen 3 in Eimeria tenella.

Eimeria tenella is an obligate intracellular parasite in the phylum Apicomplexa. As described for other members of Apicomplexa, apical membrane antigen 1 (AMA1) has been shown to be critical for sporozoite invasion of host cells by E. tenella.

Molecular characterization of 60S ribosomal protein L12 of E. tenella.

This study analyzed the large-subunit (60S) ribosomal protein L12 of Eimeria tenella (Et60s-RPL12). A full-length cDNA was cloned, and the recombinant protein was expressed in E. coli BL21 and inoculated in rabbits to produce the polyclonal antibody.

Eimeria tenella Eimeria-specific protein that interacts with apical membrane antigen 1 (EtAMA1) is involved in host cell invasion.

Background: Avian coccidiosis is a widespread, economically significant disease of poultry, caused by several Eimeria species. These parasites have complex and diverse life-cycles that require invasion of their host cells. This is mediated by various proteins secreted from apical secretory organelles.

Molecular characterization of serine/threonine protein phosphatase of Eimeria tenella.

Avian coccidiosis is a widespread and economically significant poultry disease caused by several Eimeria species, including Eimeria tenella. Previously, E. tenella serine/threonine protein phosphatase (EtSTP) was found to be differentially expressed in drug-sensitive (DS) and drug-resistant strains using RNA-seq.

[Final evaluation of medium- and long-term program of schistosomiasis control in Baise City].

Objective: To evaluate the monitoring results of medium- and long-term program of schistosomiasis control in Baise City, so as to provide the reference for the elimination of schistosomiasis.

Methods: The data of schistosomiasis control in Baise City from 2004 to 2015 were collected and analyzed.

Results: By the end of 2015, the total number of regular screening serum tests for schistosomiasis in the planning period was 10 244 person-times, with 649 positive cases.

[Analysis of inspection certification results on malaria elimination in Baise City].

To analyze the results of inspection certification on malaria elimination in Baise City, and summarize the experiences of malaria elimination at a prefecture level. According to (2014 edition) and (2016 edition), the malaria elimination work in Baise City was evaluated. In addition, 2 counties (cities, districts) in the administrative region of Baise City were randomly selected for review assessment.

[Investigation of a case of foreign imported falciparum malaria in Xilin County, Baise City].

Objective: To investigate the epidemiology, diagnosis and treatment of an imported falciparum malaria patient in Xilin County, Baise City, so as to provide the reference for improving the diagnosis and treatment of falciparum malaria patients in the future.

Methods: The epidemiological and clinical data were collected and analyzed.

Results: The patient had lived in Africa where the malaria was epidemic.

[Examination and evaluation on malaria elimination in Baise City].

Objective: To analyze the assessment results and summary the work experience of malaria elimination in Baise City.

Methods: According to (), the examination and evaluation of malaria elimination were carried out and all the results were analyzed in 12 counties (county-level city or district) of Baise City from 2014 to 2015.

Results: Since 2009, there were no local malaria cases and imported secondary cases in Baise City for the 6 consecutive years, and the detailed data were collected and the self-assessment reports of malaria elimination were written in all the counties.

[Analysis of malaria epidemic situation in Pingguo County, Guangxi Zhuang Autonomous Region from 1951 to 2014].

Objective: To explore the law and distribution characteristics of malaria prevalence in Pingguo County, Guangxi Zhuang Autonomous Region, so as to provide the evidence for formulating the targeted control strategy and measures after malaria elimination.

Methods: The data of malaria epidemic situation in Pingguo County from 1951 to 2014 were collected and analyzed with the retrospective epidemiological method.

Results: From 1951 to 2014, there were 71 365 malaria cases reported in the county.

Which is the safer method of labor induction for oligohydramnios women? Transcervical double balloon catheter or dinoprostone vaginal insert.

Objective: To compare the effectiveness and safety of two cervical ripening methods in term primiparous women with unfavorable cervices and oligohydramnios.

Methods: Women (126 cases) with oligohydramnios [amniotic fluid index (AFI) ≤5 cm] and a low Bishop Score (≤6) were assigned randomly to use double balloon catheter (mechanical method group, 67 cases) or dinoprostone 10 mg controlled-release vaginal insert (pharmacological method group, 59 cases) for induction of labor. The study's primary outcome was caesarean section rate (CSR).