Publications by authors named "Shuihong Zhou"
The importance of vocal cord leukoplakia (VCL) in the etiology and progression of laryngeal carcinoma has gained increasing recognition. However, research into the mechanisms of laryngeal precancerous lesions such as VCL, has been hampered by the small size of VCL epithelial cells and their limited culture lifespan. In this study, we enhanced the primary culture protocol for VCL epithelial cells and introduced simian virus 40 Large T to establish an immortalized cell line, designated hVCL-MSDEP01.
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- - This study evaluated the expression of glucose transporter (GLUT)-1 and hexokinase (HK)-II in vocal cord leukoplakia (VCL) and explored its clinical importance using samples from various throat conditions over a four-year period.
- - Results showed significant differences in factors like sex and smoking between VCL and vocal cord polyp patients, with GLUT-1 and HK-II expressions being higher in laryngeal carcinoma cases, particularly in severe dysplasia.
- - Both GLUT-1 and HK-II levels increased with the severity of dysplasia, indicating that their expression could serve as biomarkers for assessing the progression of VCL and its potential transformation to cancer.
Acidic Pepsin Affects Laryngeal Carcinoma Cell Growth and Invasion Through Glycolysis.
Otolaryngol Head Neck Surg
November 2024
Article Synopsis
- The study aimed to investigate the impact of acidic pepsin on Glut-1 expression and glycolytic activity in laryngeal cancer cells, focusing on its role in cell growth and migration.
- Researchers treated laryngeal carcinoma cells with acidic pepsin and evaluated various metrics including Glut-1 levels, glucose uptake, and cell viability using multiple assays.
- Results revealed that acidic pepsin significantly increased Glut-1 expression and glycolytic activity, promoting cancer cell growth and migration, effects that could be reversed with a glycolytic inhibitor and Glut-1 siRNA.
Article Synopsis
- Primary nasal tuberculosis is a rare form of TB that can occur in patients on anti-TNF immunotherapy, making diagnosis difficult.
- A 58-year-old man with a history of psoriatic arthritis experienced nasal obstruction and bloody discharge, leading to a biopsy that indicated primary nasal TB.
- Successful treatment with oral anti-TB medications resulted in the disappearance of lesions, highlighting the need to consider primary nasal TB in similar cases.
Article Synopsis
- This study collected data from over 35,000 COVID-19 patients in China to analyze the rates of olfactory (smell) and gustatory (taste) dysfunctions following infection.!
- Findings revealed a 67.75% incidence rate of these dysfunctions, with factors like gender, smoking, vaccination doses, and oral health affecting both the likelihood of experiencing and recovering from these symptoms.!
- On average, smell and taste scores declined significantly after infection but showed some recovery over time, with 59.16% of patients reporting improvement, though some experienced prolonged symptoms lasting over 28 days.!
Parapharyngeal and retropharyngeal infections in children: Kawasaki disease needs vigilance.
Braz J Otorhinolaryngol
May 2024
Article Synopsis
- - Kawasaki Disease (KD) can be easily mistaken for Parapharyngeal and Retropharyngeal Infections (PPI/RPI), causing potential misdiagnosis as Deep Neck Infections (DNIs), which require different treatment strategies and can lead to serious complications if not properly addressed.
- - In a study of 56 children, 39.3% were diagnosed with KD, showing higher fever and elevated liver enzymes compared to those diagnosed with DNIs, who often presented with deep neck abscesses.
- - Treatment for KD mimicking DNIs showed no improvement with antibiotics, but significant symptom relief was achieved using Immunoglobulin (IVIG) and aspirin, highlighting the importance of accurate diagnosis in pediatric cases.
Article Synopsis
- Upregulation of PD-1/PD-L1 lets cancer cells evade the immune system by disrupting T-cell function, making it challenging for immunotherapy to be effective in many patients with advanced cancer.
- Radiotherapy can enhance the antitumor effects by promoting T-cell recruitment and increasing PD-L1 expression, while also altering tumor metabolism, which is linked to cancer immune evasion.
- PET/CT imaging offers a non-invasive way to monitor PD-1/PD-L1 levels in tumors, providing advantages in tracking these markers over traditional immunohistochemistry methods to better inform treatment strategies.
Background: The definition of "collision tumor" is the coexistence of two histologically and morphologically distinct tumors within the same anatomical area without histological admixture. Collision tumors featuring primary squamous cell and adenoid cystic carcinomas of the hypopharynx, combined with synchronous esophageal carcinoma, are very rare.
Methods: We describe a patient with a collision tumor of the hypopharynx and synchronous esophageal carcinoma who underwent partial laryngectomy, with preservation of laryngeal function, and radical esophageal resection featuring esophageal reconstruction using a gastric tube.
Article Synopsis
- The study explores the role of mitophagy in acquired cholesteatoma, a condition that shows characteristics similar to cancer.
- Researchers analyzed specific autophagy and mitophagy protein markers in cholesteatoma epithelial cells compared to normal ear canal cells using various methods, including immunoblotting and electron microscopy.
- Results indicated increased mitophagy activity in cholesteatoma cells, suggesting that this process may contribute to the development of cholesteatoma.
Pepsin Increases the Proliferation of Vocal Cord Leukoplakia Epithelial Cells by Inducing Autophagy.
Otolaryngol Head Neck Surg
November 2023
Article Synopsis
- The study aimed to explore how H/K ATPase affects the growth of pepsin-induced vocal cord leukoplakia (VCL) cells.
- Researchers used immunohistochemistry to analyze the presence of pepsin and H/K ATPase subunits in VCL cells with different levels of cell abnormality.
- The findings revealed that higher levels of pepsin and H/K ATPase were linked to greater dysplasia in VCL, with acidified pepsin increasing cell proliferation and autophagy, which were modulated by certain inhibitors.
Article Synopsis
- Thyroglossal duct cysts (TDCs) are usually single cysts, but this study describes an unusual case with five cysts, which is the first of its kind reported in the front neck area.
- The treatment involved a Sistrunk operation for complete excision, and histological analysis confirmed the presence of TDCs with no recurrence after 6 years.
- The case highlights the rarity of multiple TDCs and emphasizes the need for thorough preoperative imaging and careful assessment to avoid misdiagnosis.
Objectives: Despite the increasingly modern surgical techniques in the oncology field, the factors that influence postoperative prognosis in patients with hypopharyngeal and laryngeal carcinoma (HLC) remain unclear. The study aimed to evaluate the factors influencing the prognosis of HLC patients with pathological diagnosis of squamous cell carcinoma, and the findings are intended to direct follow-up management strategies.
Methods: A retrospective cohort study was performed.
Purpose: The mechanism underlying malignant transformation of vocal fold leukoplakia (VFL) and the precise role of the expression of pepsin in VFL remain unclear. This study aimed to investigate the effects of acidified pepsin on VFL epithelial cell growth and migration, and also identify pertinent molecular mechanisms.
Methods: Immunochemistry and Western blotting were performed to measure glucose transporter type 1 (GLUT1), monocarboxylate transporters 4 (MCT4), and Hexokinase-II (HK-II) expressions.
Article Synopsis
- * Analysis of tissue samples showed that high levels of stromal MCT4 and TOMM20 are linked to worse prognosis, with MCT4 identified as a key independent prognostic factor.
- * Findings suggest that metabolic symbiosis occurs in laryngeal cancer tissues, indicating that TOMM20 and stromal MCT4 could be promising targets for new treatments.
Article Synopsis
- The study focused on the clinical features, treatment options, and prognosis of laryngeal leiomyosarcoma (LLMS), particularly looking at cases associated with Epstein-Barr virus (EBV) and HIV infections.
- It reported on a specific case of EBV-associated LLMS in an adult HIV patient and reviewed existing literature, finding that only 62 cases have been documented.
- The findings showed that LLMS has a 5-year survival rate of 64%, while EBV association seems to negatively impact survival rates, with EBV-positive patients showing a lower rate of 50%.
Article Synopsis
- Ingested foreign bodies can sometimes move to the paraglottic space, requiring surgical intervention for removal.
- A case is discussed where an ingested fishbone was successfully extracted using a transcervical approach.
- The posterolateral approach is highlighted as a safe and effective method for removing deeply embedded foreign objects in this area.
Article Synopsis
- A study investigated the knowledge of laryngopharyngeal reflux disease (LPRD) among Chinese otolaryngologists through a cross-sectional survey involving 2,254 participants across 220 medical centers.
- The results showed that 96.4% of participants had heard of LPRD, primarily learning about it from academic conferences, but only a small percentage were aware of the most accurate diagnostic methods and comprehensive treatment options.
- Overall, while otolaryngologists demonstrated some awareness regarding LPRD, there is a significant need for improved education and resources to enhance their understanding of the disease.
Article Synopsis
- A thyroglossal duct cyst is a common congenital neck condition, typically found in children and adults, usually presenting as a midline neck mass.
- A rare case involved a 64-year-old man who only showed hoarseness as a symptom, with no visible neck mass.
- Surgery successfully removed the cyst, confirming the diagnosis, and the patient’s voice returned to normal, highlighting the need for careful imaging in atypical cases.
Article Synopsis
- The text discusses a case of laryngeal angioleiomyoma, a rare benign tumor in the larynx, emphasizing its characteristics and management strategies for better diagnosis and treatment.
- It reports on the oldest known patient with this condition and reviews a total of 36 cases documented in both English and Chinese literature, noting a male-to-female ratio of 5:1 and an average age of nearly 54 years.
- The study highlights that the supraglottic region is the most common site for these tumors, warns against performing biopsies due to the risk of bleeding, and states that complete surgical resection is the preferred treatment with very low chances of recurrence or malignant transformation.
Background: Papillary thyroid carcinoma (PTC) grows slowly but has a great risk of metastasis. MicroRNAs are well known as vital tumor-related gene regulators. In PTC, the role of miR-203a-3p and the underlying mechanisms remain not completely understood.
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- * This study identified that hypoxia increases radioresistance in Tu212 cells through enhanced glucose uptake and activation of the PI3K/Akt/mTOR pathway, and inhibiting this pathway can reverse the effects of hypoxia.
- * Knocking out HIF-1α and/or Glut-1 not only lowered cell viability and glucose absorption but also enhanced radiosensitivity in tumor cells, leading to reduced tumor growth and increased cell apoptosis.
Article Synopsis
- * LCS typically affects multiple organs such as skin, lymph nodes, lungs, and bone, and factors like immunosuppression, viral infections, and previous blood disorders may contribute to its development.
- * This case report is notable as it describes LCS associated with Epstein-Barr virus (EBV) infection, presenting initially with bilateral cervical giant cysts, prompting new considerations regarding EBV's potential role in cancer development.
Article Synopsis
- * A mouse model simulating laryngopharyngeal reflux was used to observe changes in the laryngeal epithelium after treatment with pepsin, revealing significant cell proliferation over time.
- * Results indicated that blocking Glut-1 reduced cell growth, and the study found increased expression of Glut-1 and H/K-ATPase proteins following pepsin exposure, suggesting their roles in laryngeal hyperplasia.
Article Synopsis
- - The study investigates the role of the PI3K/Akt/HIF-1 pathway and IL-17A in the unique pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) in a Chinese population.
- - Researchers measured various inflammatory markers and protein expressions in nasal polyps, finding elevated levels of MPO activity, PI3K, p-Akt, HIF-1, and IL-17A mRNA in patients with CRSwNP.
- - The results suggest that the PI3K/Akt/HIF-1 pathway contributes to CRSwNP's development, and that increased IL-17A levels may be linked to neutrophilic inflammation in the condition.
Article Synopsis
- * Recent research has started to recognize the importance of tumor stroma, particularly fibroblasts, in cancer development and progression beyond just the epithelial cells.
- * This study aims to review the effects of tobacco on fibroblasts and examine how these interactions influence cancer development.