Publications by authors named "Shui-xiu Li"
Article Synopsis
- Previous research connected mitochondrial biology to fungal pathogenicity, focusing on the FF-ATP synthase β subunit, which plays a crucial role in energy production.
- A gene null mutant (Δ/Δ) was created to analyze the effects of this subunit on carbon metabolism and pathogenicity, revealing that the mutant struggled with growth and cell viability, especially on non-fermentable carbon sources.
- The findings indicate that the FF-ATP synthase β subunit is essential for fungal pathogenicity by facilitating metabolic flexibility, impacting energy production and virulence in infection scenarios.
Article Synopsis
- * The study compares mutant strains (Δ/Δ) and gene-reconstituted strains (Δ) with wild type (WT) in a mouse model, finding that Δ/Δ has a higher survival rate and lower fungal burden in infected mice.
- * Key findings reveal that Δ/Δ strains struggle to damage immune cells, grow under stress conditions, and exhibit defects in important processes like filamentation and biofilm formation, resulting in significantly lower ATP levels compared to WT.
Synergistic Effects of Tetrandrine with Posaconazole Against Aspergillus fumigatus.
Microb Drug Resist
September 2017
In our earlier in vitro and in vivo studies, synergistic effects were observed when itraconazole or voriconazole were combined with tetrandrine (TET) against Aspergillus fumigatus, and the synergistic mechanism was related to inhibition of the drug efflux pump. Posaconazole (PCZ) is a broad-spectrum triazole antifungal agent used for the treatment of diverse fungal infections, including aspergillosis and candidiasis. Herein, the antifungal effects of TET are further investigated in vitro and in vivo alone or combined with PCZ against 20 clinical isolates of A.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to assess the toxicity effects of intravenously administered tetrandrine (TET) in female BALB/c mice, focusing on both acute and sub-chronic exposure.
- The acute toxicity tests found no significant harmful effects at single doses of TET ranging from 20 to 340 mg/kg, while the sub-acute tests revealed potential toxicity at 150 mg/kg, particularly affecting the liver, lungs, and kidneys, but these effects were reversible after stopping TET.
- Overall, the findings suggest that TET has a relatively safe profile, with doses up to 90 mg/kg being non-toxic over 14 days of administration.
In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A.
View Article and Find Full Text PDFWe found that tetrandrine (TET) can reverse the resistance of Candida albicans to fluconazole (FLC) and that this interaction is associated with the inhibition of drug efflux pumps. Mitochondrial aerobic respiration, which plays a major role in C. albicans metabolism, is the primary source of ATP for cellular processes, including the activation of efflux pumps.
View Article and Find Full Text PDFBackground: The most critical mechanism governing drug resistance in Candida albicans (C. albicans) involves efflux pumps, the functionality of which largely depends on energy metabolism. Alcohol dehydrogenase I (ADH1) plays an important role in intracellular energy metabolism.
View Article and Find Full Text PDF