[Expression of vascular growth factors in intestinal tissues in colorectal carcinoma patients with schistosomiasis japonica].

Objective: To investigate the differences of mRNA quantitation and protein expression of vascular growth factors including platelet-derived endothelial cell growth factor (PD-ECGF) and vascular endothelial growth factor (VEGF) in intestinal tissues in colorectal carcinoma patients with and without schistosomiasis.

Methods: Thirty colorectal carcinoma patients with schistosomiasis and 30 colorectal carcinoma patients without schistosomiasis were included in this study. The mRNA quantitation and protein expression of PD-ECGF and VEGF in the normal tissue, peri-carcinoma tissue as well as carcinoma tissue obtained from surgical specimens were detected by qRT-PCR and Western blot.

[Role of Toll-like receptor 2/4-nuclear factor-κB signaling pathway in invasion of Mycobacterium tuberculosis to mouse dendritic cells].

Objective: To investigate the mechanism of Mycobacterium tuberculosis invasion to mouse dendritic cells (DC).

Methods: Mycobacterium tuberculosis strain H37Rv was co-cultured with mouse DC2.4 cells.

Localization of Vibrio vulnificus infection in dendritic cells and its effects on the cytoskeleton.

Background: Vibrio vulnificus (Vv) is an estuarine bacterium that can cause primary septicemia as well as serious wound infections. However, little is known about the mechanisms by which Vv infects dendritic cells (DCs) and its effects on cytoskeleton. In this study, we aimed to investigate the invasion, internalization, and the organelles damage of the cultured dendritic cells (a DC 2.

Genome sequence of the human-pathogenetic bacterium Vibrio vulnificus B2.

Vibrio vulnificus, which can lead to rapidly expanding cellulitis or septicemia, is present in the marine environment. Here, we present the draft genome sequence of strain B2, which was isolated from a septicemia patient in 2010.

[Effects of snakegourd root polysaccharide on apoptosis of MCF-7 cells].

Objective: To investigate the effects of snakegourd root polysaccharide on apoptosis of human breast cancer cells (MCF-7 cells).

Methods: Colorimetric MTT assay was used to measure the inhibition of snakegourd root polysaccharide on MCF-7 cells. The morphological changes of MCF-7 cells were observed by fluorescence microscope after DAPI staining and transmission electron microscope.

[Effects of Mycobacterium tuberculosis on apoptosis of mouse dendritic cells and activation of caspase-3, caspase-8].

Objective: To investigate the effects of Mycobacterium tuberculosis on apoptosis of mouse dendritic cells (DC 2. 4) and the activation of caspase-3, caspase-8.

Methods: Mycobacterium tuberculosis H37Rv strain was co-cultured with DC 2.

[Cytoskeleton rearrangements and induced apoptosis in human umbilical venous endothelial cell and WISH cell lines during the invasion of Staphylococcus aureus].

Objective: To investigate the changes of cytoskeleton and induced apoptosis in human umbilical venous endothelial cells (HUVEC) and WISH cells during the invasion of Staphylococcus aureus.

Methods: S. aureus suspension was collected routinely and used to infect HUVEC and WISH cells for 10, 30, 60 and 120 min respectively.

Antisense expression of PKCalpha improved sensitivity of SGC7901/VCR cells to doxorubicin.

Aim: To explore whether antisense blocking of protein kinase C alpha (PKCalpha) would reverse multi-drug resistance (MDR) in the vincristine (VCR)-resistant human gastric cancer cell line SGC7901/VCR.

Methods: SGC7901/VCR cells expressing antisense PKCalpha, SGC7901/VCR/aPKC, were established by transfection with a recombinant plasmid reversely inserted with PKCalpha cDNA. Empty vector (PCI-neo)-transfected cell clones, SGC7901/VCR/neo, served as the control.